Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BJLGFPMG_4 | 708535 | 709704 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| BJLGFPMG_4 | 480478 | 481680 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| BJLGFPMG_4 | 461649 | 462503 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| BJLGFPMG_3 | 429052 | 429369 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BJLGFPMG_3 | 428714 | 429052 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BJLGFPMG_1 | 108152 | 109072 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| BJLGFPMG_1 | 94816 | 96255 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| BJLGFPMG_1 | 45137 | 45730 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BJLGFPMG_6 | 29197 | 30840 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| BJLGFPMG_2 | 27322 | 29892 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BJLGFPMG_4 | 461649 | 462501 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| BJLGFPMG_5 | 39263 | 40639 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.62 | 100.00 | D12567 |
| BJLGFPMG_1 | 108152 | 109072 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BJLGFPMG_1 | 108155 | 109072 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| BJLGFPMG_5 | 39263 | 40636 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| BJLGFPMG_6 | 29197 | 30837 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| BJLGFPMG_4 | 461649 | 462500 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| BJLGFPMG_5 | 42642 | 43160 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| BJLGFPMG_4 | 1011299 | 1013350 | rphC | rifamycin-inactivating phosphotransferase RphC | PARTIALX | 80.12 | 79.03 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BJLGFPMG_4 | 908231 | 908872 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| BJLGFPMG_5 | 512508 | 513689 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| BJLGFPMG_5 | 927278 | 928063 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| BJLGFPMG_5 | 930961 | 931899 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| BJLGFPMG_5 | 514172 | 515314 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| BJLGFPMG_5 | 931919 | 939053 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.34 | 99.97 | NP_391076 |
| BJLGFPMG_5 | 513704 | 514153 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BJLGFPMG_4 | 1061668 | 1063431 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| BJLGFPMG_5 | 928089 | 929285 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| BJLGFPMG_5 | 929314 | 930933 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BJLGFPMG_9 | 27943 | 26807 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BJLGFPMG_13 | 4285 | 5154 | pdxS | 2.58e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BJLGFPMG_3 | 739242 | 740018 | CodY | 7.67e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BJLGFPMG_5 | 675049 | 674480 | ClpP | 5.66e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BJLGFPMG_3 | 276507 | 276899 | spxA1 | 2.27e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| BJLGFPMG_10 | 34869 | 34627 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BJLGFPMG_6 | 80340 | 80143 | CspA | 9.69e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BJLGFPMG_1 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_2 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_3 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_4 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_5 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_6 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_7 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_8 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_9 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_10 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_12 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_13 | 0.0 | 1.0 | 0.0 |
| BJLGFPMG_14 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BJLGFPMG_3 | 371338 | 399227 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| BJLGFPMG_4 | 501665 | 531514 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG4673, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)