safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	32	46.69

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
IPMEIMAE_1	394	1233	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
IPMEIMAE_11	14282	15072	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	100.00	100.00	-
IPMEIMAE_2	5123	5974	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
IPMEIMAE_2	183905	184666	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
IPMEIMAE_11	90412	91335	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
IPMEIMAE_11	89502	90452	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
IPMEIMAE_11	88395	89477	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
IPMEIMAE_11	87331	88305	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
IPMEIMAE_11	86846	87325	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
IPMEIMAE_11	85535	86653	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
IPMEIMAE_11	84008	84967	STR_RS05240	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827042
IPMEIMAE_11	83245	83844	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
IPMEIMAE_11	82359	83108	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
IPMEIMAE_11	81657	82349	STR_RS05255	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227247
IPMEIMAE_11	80917	81648	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
IPMEIMAE_11	79462	80916	STR_RS05265	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827043
IPMEIMAE_2	219430	220023	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
IPMEIMAE_2	16707	18452	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
IPMEIMAE_2	15005	16705	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
IPMEIMAE_2	13785	14987	STR_RS06895	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227428
IPMEIMAE_2	12979	13785	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
IPMEIMAE_2	12038	12979	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
IPMEIMAE_2	10893	12041	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
IPMEIMAE_11	94458	95516	waaB	0.0	Capsule	Immune modulation	100.00	100.00	WP_157822584
IPMEIMAE_10	74831	76489	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
IPMEIMAE_11	91343	92764	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
IPMEIMAE_2	219430	220023	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
IPMEIMAE_2	219430	220023	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
IPMEIMAE_2	15005	16705	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
IPMEIMAE_2	12979	13785	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
IPMEIMAE_2	12038	12979	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
IPMEIMAE_2	13785	14987	STER_RS07055	0.0	Capsule	Immune modulation	99.42	100.00	WP_011681436
IPMEIMAE_11	81657	82349	STU_RS14640	0.0	Capsule	Immune modulation	99.42	100.00	WP_011226047
IPMEIMAE_2	10893	12041	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
IPMEIMAE_2	218561	219430	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
IPMEIMAE_11	80917	81648	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
IPMEIMAE_11	82359	83094	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
IPMEIMAE_11	80917	81648	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
IPMEIMAE_2	16707	18452	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
IPMEIMAE_11	82359	83094	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
IPMEIMAE_11	79462	80916	STU_RS14650	0.0	Capsule	Immune modulation	97.94	100.00	WP_011226049
IPMEIMAE_2	6095	7354	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
IPMEIMAE_11	79462	80916	STER_RS05320	0.0	Capsule	Immune modulation	96.92	99.79	WP_011681177
IPMEIMAE_11	81657	82349	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
IPMEIMAE_1	266011	267314	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
IPMEIMAE_6	107126	108136	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
IPMEIMAE_2	12979	13785	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
IPMEIMAE_9	6714	7997	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
IPMEIMAE_2	220452	221498	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
IPMEIMAE_2	7351	8049	STER_RS07095	4.08e-153	Capsule	Immune modulation	81.15	70.35	WP_011681444
IPMEIMAE_11	85023	85259	STR_RS05235*	3.54e-120	Capsule	Immune modulation	100.00	100.00	-
IPMEIMAE_2	8403	8869	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
IPMEIMAE_2	13785	14522	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IPMEIMAE_2	181218	180235	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IPMEIMAE_8	14009	12936	MntE	0.0	manganese homeostasis	94.13	99.72	ABF32167
IPMEIMAE_1	183775	184743	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
IPMEIMAE_3	10471	8168	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IPMEIMAE_2	94357	92876	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
IPMEIMAE_16	11322	9076	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
IPMEIMAE_1	20025	21017	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
IPMEIMAE_17	15698	14727	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IPMEIMAE_3	124302	125453	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
IPMEIMAE_3	158775	160757	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
IPMEIMAE_2	197412	199142	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.69	100.00	AAK34199
IPMEIMAE_7	63225	61747	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
IPMEIMAE_1	266011	267312	Eno	0.0	enolase	86.21	99.77	ABP90468
IPMEIMAE_7	48489	46597	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
IPMEIMAE_16	6151	7860	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
IPMEIMAE_5	79960	78950	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IPMEIMAE_12	44707	46068	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
IPMEIMAE_5	29629	31521	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
IPMEIMAE_1	251581	252471	bglB	5.01e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
IPMEIMAE_10	13471	12536	SP_0676	3.55e-169	transcription facot	81.73	96.89	AAK74821
IPMEIMAE_16	8884	8027	metF	1.17e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
IPMEIMAE_11	42824	43528	covR	1.81e-131	responder protein	84.68	100.00	AAK71319
IPMEIMAE_2	128036	128707	rr01	1.61e-126	response regulator	84.38	99.56	CAB54566
IPMEIMAE_11	42824	43528	walR	1.30e-121	DNA-binding response regulator	80.85	100.00	ANC99504
IPMEIMAE_4	58484	59170	CovR	5.87e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IPMEIMAE_16	2198	1548	CiaR	1.89e-118	two-component response regulator	86.17	96.88	AAK74935
IPMEIMAE_16	2198	1548	ciaR	9.72e-116	two component system response regulator	83.87	96.02	AIK75708
IPMEIMAE_1	21314	21709	SpxA2	6.38e-75	transcriptional regulator	90.91	100.00	ERL21461
IPMEIMAE_4	83046	83333	spr0479	9.40e-49	essential gene	86.46	98.97	AAK99283
IPMEIMAE_1	149099	149353	spr1327	4.76e-45	essential gene	95.29	100.00	AAL00131
IPMEIMAE_7	14845	15108	spr0175	4.30e-42	pathogenitcity related	84.09	100.00	AAK98979
IPMEIMAE_7	15672	15938	spr0177	1.11e-33	essential gene	84.27	88.12	AAK98981
IPMEIMAE_9	8155	8655	RopE (M5005_Spy_1611)	3.78e-76	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
IPMEIMAE_3	10471	8174	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IPMEIMAE_5	44090	45109	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IPMEIMAE_1	0.0	1.0	0.0
IPMEIMAE_2	0.0	1.0	0.0
IPMEIMAE_3	0.0	1.0	0.0
IPMEIMAE_4	0.0	1.0	0.0
IPMEIMAE_5	0.0	1.0	0.0
IPMEIMAE_6	0.0	1.0	0.0
IPMEIMAE_7	0.0	1.0	0.0
IPMEIMAE_8	0.0	1.0	0.0
IPMEIMAE_9	0.0	1.0	0.0
IPMEIMAE_10	0.0	1.0	0.0
IPMEIMAE_11	0.0	1.0	0.0
IPMEIMAE_12	0.0	1.0	0.0
IPMEIMAE_13	0.0	1.0	0.0
IPMEIMAE_14	0.0	1.0	0.0
IPMEIMAE_15	0.0	1.0	0.0
IPMEIMAE_16	0.0	1.0	0.0
IPMEIMAE_17	0.0	1.0	0.0
IPMEIMAE_18	0.0	1.0	0.0
IPMEIMAE_19	0.0	1.0	0.0
IPMEIMAE_20	0.0	1.0	0.0
IPMEIMAE_21	1.0	0.0	1.0
IPMEIMAE_22	0.0	1.0	0.0
IPMEIMAE_23	0.0	1.0	0.0
IPMEIMAE_24	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found