Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKFNBHAP_1 | 1406585 | 1407754 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BKFNBHAP_2 | 464307 | 465509 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| BKFNBHAP_2 | 447557 | 448414 | aadK | ARO:3002627 | aadK | 97.18 | 100.35 | AL009126.1 |
| BKFNBHAP_1 | 426553 | 426870 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BKFNBHAP_1 | 426215 | 426553 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BKFNBHAP_5 | 109248 | 110168 | mphK | ARO:3004541 | mphK | 99.01 | 100.00 | CP010314.1 |
| BKFNBHAP_5 | 95914 | 97353 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| BKFNBHAP_5 | 46264 | 46857 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BKFNBHAP_7 | 28897 | 30543 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| BKFNBHAP_9 | 27410 | 29980 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BKFNBHAP_5 | 109248 | 110168 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| BKFNBHAP_3 | 37463 | 38839 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.47 | 100.00 | D12567 |
| BKFNBHAP_2 | 447557 | 448408 | aadK | Streptomycin | 98.24 | 99.65 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKFNBHAP_5 | 109260 | 110168 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.01 | 99.02 | WP_003246254.1 |
| BKFNBHAP_3 | 37463 | 38836 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.25 | 100.00 | WP_063856081.1 |
| BKFNBHAP_7 | 28897 | 30540 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| BKFNBHAP_2 | 447557 | 448408 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| BKFNBHAP_3 | 40813 | 41331 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| BKFNBHAP_1 | 1112544 | 1115138 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BKFNBHAP_1 | 1209085 | 1209726 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| BKFNBHAP_3 | 536279 | 537460 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| BKFNBHAP_4 | 152420 | 153205 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BKFNBHAP_4 | 151198 | 152394 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| BKFNBHAP_4 | 148584 | 149522 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| BKFNBHAP_3 | 537475 | 537924 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| BKFNBHAP_1 | 1063052 | 1064815 | dep/capD | 0.0 | Capsule | Immune modulation | 98.81 | 100.00 | NP_389723 |
| BKFNBHAP_3 | 537943 | 539085 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| BKFNBHAP_4 | 149550 | 151169 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.39 | 100.00 | NP_391078 |
| BKFNBHAP_4 | 141428 | 148564 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.39 | 100.00 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BKFNBHAP_11 | 28040 | 26904 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BKFNBHAP_12 | 4415 | 5284 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BKFNBHAP_1 | 737212 | 737988 | CodY | 1.20e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BKFNBHAP_3 | 693392 | 692823 | ClpP | 4.74e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BKFNBHAP_1 | 276396 | 276788 | spxA1 | 9.42e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BKFNBHAP_1 | 276396 | 276788 | spx | 5.62e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BKFNBHAP_10 | 20679 | 20921 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BKFNBHAP_7 | 84415 | 84218 | CspA | 1.04e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BKFNBHAP_1 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_2 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_3 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_4 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_5 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_6 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_7 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_9 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_10 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_11 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_12 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_13 | 0.0 | 1.0 | 0.0 |
| BKFNBHAP_16 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BKFNBHAP_1 | 370304 | 396665 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| BKFNBHAP_4 | 20723 | 41774 | False | Siphoviridae | VOG0753, VOG5535, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG0698, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796, VOG7983, VOG0198, VOG7007 |
| BKFNBHAP_4 | 46953 | 51978 | False | Unknown | VOG4566, VOG0186, VOG8233, VOG1878, VOG4552 |
Phigaro v2.4.0 (Database: Jan. 2024 release)