Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EMHILBAA_7 | 53780 | 54829 | clbA | ARO:3002814 | clbA | 93.12 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EMHILBAA_7 | 53780 | 54829 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.77 | 99.90 | KR610408 |
| EMHILBAA_2 | 357862 | 359238 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.73 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EMHILBAA_7 | 53783 | 54829 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 93.12 | 100.00 | WP_012116915.1 |
| EMHILBAA_2 | 357862 | 359232 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 87.53 | 99.78 | WP_003242953.1 |
| EMHILBAA_7 | 56610 | 57128 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 84.39 | 100.00 | WP_003242546.1 |
| EMHILBAA_5 | 950022 | 952619 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EMHILBAA_3 | 474733 | 475914 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| EMHILBAA_3 | 475929 | 476378 | capC | 3.64e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EMHILBAA_8 | 27785 | 26649 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| EMHILBAA_11 | 12527 | 11658 | pdxS | 6.53e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| EMHILBAA_5 | 751966 | 752742 | CodY | 8.97e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EMHILBAA_5 | 262994 | 263386 | spxA1 | 1.42e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| EMHILBAA_7 | 71309 | 71112 | CspA | 5.89e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| EMHILBAA_6 | 239012 | 238818 | CspD | 9.80e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| EMHILBAA_6 | 239012 | 238815 | CspB | 2.03e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| EMHILBAA_7 | 71309 | 71115 | CspR | 2.92e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EMHILBAA_1 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_2 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_3 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_4 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_5 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_6 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_7 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_8 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_10 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_11 | 0.0 | 1.0 | 0.0 |
| EMHILBAA_15 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EMHILBAA_5 | 347430 | 373214 | False | Myoviridae | VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG4545, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG7895 |
| EMHILBAA_6 | 156308 | 168000 | False | Myoviridae / Siphoviridae | VOG9957, VOG0532, VOG0052, VOG0026, VOG7462, VOG4966, VOG4724, VOG4658, VOG9507 |
| EMHILBAA_6 | 179901 | 208632 | False | Siphoviridae | VOG4581, VOG1886, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0136, VOG4707, VOG8440, VOG6116 |
Phigaro v2.4.0 (Database: Jan. 2024 release)