Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MFOHPLNP_5 | 515202 | 515540 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MFOHPLNP_5 | 514885 | 515202 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MFOHPLNP_3 | 466051 | 466905 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| MFOHPLNP_3 | 446878 | 448080 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| MFOHPLNP_3 | 220241 | 221410 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MFOHPLNP_4 | 108076 | 108996 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| MFOHPLNP_4 | 94741 | 96180 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| MFOHPLNP_1 | 29011 | 30654 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| MFOHPLNP_7 | 27533 | 30103 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MFOHPLNP_6 | 994378 | 995754 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
| MFOHPLNP_3 | 466053 | 466905 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| MFOHPLNP_4 | 108076 | 108996 | mph(K) | Spiramycin, Telithromycin | 97.50 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MFOHPLNP_6 | 994381 | 995754 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| MFOHPLNP_1 | 29011 | 30651 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| MFOHPLNP_4 | 108079 | 108996 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| MFOHPLNP_3 | 466054 | 466905 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| MFOHPLNP_6 | 991861 | 992379 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| MFOHPLNP_9 | 58323 | 60920 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MFOHPLNP_3 | 21054 | 21695 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| MFOHPLNP_6 | 501029 | 502210 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MFOHPLNP_9 | 8298 | 10061 | dep/capD | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_389723 |
| MFOHPLNP_6 | 86510 | 87295 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MFOHPLNP_6 | 82674 | 83612 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| MFOHPLNP_6 | 85288 | 86484 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| MFOHPLNP_6 | 75520 | 82654 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.06 | 99.97 | NP_391076 |
| MFOHPLNP_6 | 499404 | 500546 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| MFOHPLNP_6 | 500565 | 501014 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| MFOHPLNP_6 | 83640 | 85259 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MFOHPLNP_13 | 11266 | 10397 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MFOHPLNP_5 | 205304 | 204528 | CodY | 7.65e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MFOHPLNP_6 | 339675 | 340244 | ClpP | 6.20e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MFOHPLNP_5 | 667493 | 667101 | spxA1 | 6.02e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MFOHPLNP_5 | 667493 | 667101 | spx | 3.59e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MFOHPLNP_10 | 34782 | 34540 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MFOHPLNP_1 | 81466 | 81269 | CspA | 9.80e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MFOHPLNP_3 | 33657 | 33851 | CspD | 5.61e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MFOHPLNP_1 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_3 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_4 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_5 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_6 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_7 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_9 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_10 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_11 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_12 | 1.0 | 0.0 | 1.0 |
| MFOHPLNP_13 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_14 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_15 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_17 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_18 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_19 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_20 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_21 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_22 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_23 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_24 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_25 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_26 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_28 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_29 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_30 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_31 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_32 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_33 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_34 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_35 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_36 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_37 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_40 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_41 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_43 | 0.0 | 1.0 | 0.0 |
| MFOHPLNP_44 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MFOHPLNP_3 | 398410 | 428259 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
| MFOHPLNP_5 | 545028 | 572689 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| MFOHPLNP_11 | 19892 | 44926 | False | Siphoviridae | VOG8537, VOG4574, VOG7900, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG9667, VOG0275, VOG8610, VOG4705, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036 |
| MFOHPLNP_12 | 24968 | 32957 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025, VOG4551 |
Phigaro v2.4.0 (Database: Jan. 2024 release)