Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DICBFCEL_4 | 633913 | 635082 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DICBFCEL_4 | 463785 | 464987 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| DICBFCEL_4 | 445464 | 446318 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| DICBFCEL_1 | 428395 | 428712 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DICBFCEL_1 | 428057 | 428395 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DICBFCEL_3 | 403459 | 405105 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| DICBFCEL_6 | 255464 | 258034 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DICBFCEL_3 | 162321 | 162914 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| DICBFCEL_3 | 111843 | 113282 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| DICBFCEL_3 | 99034 | 99954 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DICBFCEL_2 | 1001141 | 1002517 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | X08034 |
| DICBFCEL_4 | 445464 | 446316 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| DICBFCEL_3 | 99034 | 99954 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DICBFCEL_2 | 1001144 | 1002517 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| DICBFCEL_3 | 403462 | 405105 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| DICBFCEL_4 | 445464 | 446315 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| DICBFCEL_3 | 99034 | 99951 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| DICBFCEL_2 | 998670 | 999188 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| DICBFCEL_5 | 91763 | 94360 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DICBFCEL_2 | 109274 | 110212 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| DICBFCEL_2 | 524656 | 525837 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DICBFCEL_2 | 110240 | 111859 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| DICBFCEL_2 | 113110 | 113895 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| DICBFCEL_4 | 872385 | 873026 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| DICBFCEL_2 | 523031 | 524173 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| DICBFCEL_2 | 111888 | 113084 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| DICBFCEL_5 | 139538 | 141301 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| DICBFCEL_2 | 102118 | 109254 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| DICBFCEL_2 | 524192 | 524641 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DICBFCEL_10 | 31891 | 33027 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DICBFCEL_15 | 4487 | 5356 | pdxS | 1.13e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DICBFCEL_1 | 738262 | 739038 | CodY | 7.78e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DICBFCEL_2 | 375405 | 375974 | ClpP | 6.25e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DICBFCEL_1 | 276484 | 276876 | spxA1 | 6.12e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DICBFCEL_1 | 276484 | 276876 | spx | 3.65e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DICBFCEL_11 | 20599 | 20841 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DICBFCEL_3 | 356704 | 356901 | CspA | 1.88e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DICBFCEL_4 | 860421 | 860227 | CspD | 6.19e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DICBFCEL_1 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_2 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_3 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_4 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_5 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_6 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_10 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_11 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_12 | 1.0 | 0.0 | 1.0 |
| DICBFCEL_13 | 1.0 | 0.0 | 1.0 |
| DICBFCEL_14 | 1.0 | 0.0 | 1.0 |
| DICBFCEL_15 | 0.0 | 1.0 | 0.0 |
| DICBFCEL_16 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DICBFCEL_1 | 371013 | 398577 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| DICBFCEL_4 | 903328 | 917974 | False | Unknown | VOG7900, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312 |
| DICBFCEL_4 | 976965 | 989230 | False | Siphoviridae | VOG4908, VOG6211, VOG0322, VOG7476, VOG0025, VOG4551, VOG0918 |
Phigaro v2.4.0 (Database: Jan. 2024 release)