Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKOMGDJH_1 | 695999 | 697168 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BKOMGDJH_2 | 595352 | 595690 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| BKOMGDJH_2 | 595035 | 595352 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BKOMGDJH_1 | 465688 | 466890 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| BKOMGDJH_1 | 436512 | 437366 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| BKOMGDJH_4 | 422543 | 424189 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| BKOMGDJH_7 | 266442 | 269012 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| BKOMGDJH_4 | 162029 | 162622 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| BKOMGDJH_4 | 111474 | 112913 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| BKOMGDJH_4 | 98661 | 99581 | mphK | ARO:3004541 | mphK | 96.73 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BKOMGDJH_6 | 263873 | 265249 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.42 | 100.00 | D12567 |
| BKOMGDJH_1 | 436512 | 437366 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
| BKOMGDJH_4 | 98661 | 99581 | mph(K) | Spiramycin, Telithromycin | 96.85 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKOMGDJH_6 | 263876 | 265249 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| BKOMGDJH_4 | 422546 | 424189 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| BKOMGDJH_1 | 436512 | 437363 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| BKOMGDJH_4 | 98661 | 99578 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.73 | 100.00 | WP_003246254.1 |
| BKOMGDJH_6 | 261345 | 261863 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
| BKOMGDJH_9 | 119532 | 122129 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BKOMGDJH_8 | 109358 | 110296 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| BKOMGDJH_3 | 282368 | 283549 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| BKOMGDJH_8 | 110324 | 111943 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.69 | 100.00 | NP_391078 |
| BKOMGDJH_1 | 900115 | 900756 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| BKOMGDJH_8 | 113194 | 113979 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| BKOMGDJH_3 | 280743 | 281885 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BKOMGDJH_8 | 102202 | 109338 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.27 | 100.00 | NP_391076 |
| BKOMGDJH_3 | 281904 | 282353 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BKOMGDJH_9 | 166205 | 167968 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| BKOMGDJH_8 | 111972 | 113168 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BKOMGDJH_10 | 31744 | 32880 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BKOMGDJH_12 | 11191 | 10322 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BKOMGDJH_2 | 281938 | 281162 | CodY | 1.39e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BKOMGDJH_3 | 130593 | 131162 | ClpP | 2.99e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BKOMGDJH_5 | 162044 | 161652 | spxA1 | 2.78e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BKOMGDJH_5 | 162044 | 161652 | spx | 1.66e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BKOMGDJH_11 | 20589 | 20831 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BKOMGDJH_4 | 367242 | 367439 | CspA | 1.96e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BKOMGDJH_1 | 888154 | 887960 | CspD | 6.22e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| BKOMGDJH_5 | 405278 | 405472 | CspB | 4.01e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BKOMGDJH_1 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_2 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_3 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_4 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_5 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_6 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_7 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_8 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_9 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_10 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_11 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_12 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_13 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_14 | 0.0 | 1.0 | 0.0 |
| BKOMGDJH_15 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BKOMGDJH_1 | 496782 | 520943 | False | Myoviridae | VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| BKOMGDJH_2 | 625451 | 652371 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| BKOMGDJH_5 | 113555 | 124911 | False | Siphoviridae | VOG3490, VOG0189, VOG0025, VOG4551, VOG8506, VOG8598, VOG0026, VOG5526 |
Phigaro v2.4.0 (Database: Jan. 2024 release)