Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NKFPMKOK_4 | 160099 | 161145 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NKFPMKOK_4 | 160099 | 161142 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| NKFPMKOK_8 | 186732 | 188108 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NKFPMKOK_4 | 160099 | 161142 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| NKFPMKOK_8 | 186732 | 188102 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| NKFPMKOK_4 | 157830 | 158348 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| NKFPMKOK_9 | 5238 | 7835 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NKFPMKOK_1 | 497643 | 498824 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| NKFPMKOK_1 | 497179 | 497628 | capC | 3.25e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NKFPMKOK_10 | 27529 | 26393 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| NKFPMKOK_22 | 12486 | 11617 | pdxS | 6.35e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| NKFPMKOK_7 | 126463 | 125687 | CodY | 6.80e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| NKFPMKOK_3 | 41576 | 41184 | spxA1 | 4.31e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| NKFPMKOK_4 | 143603 | 143800 | CspA | 3.19e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| NKFPMKOK_8 | 611658 | 611464 | CspD | 1.84e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| NKFPMKOK_8 | 611658 | 611461 | CspB | 3.82e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| NKFPMKOK_4 | 143603 | 143797 | CspR | 1.58e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NKFPMKOK_1 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_2 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_3 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_4 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_5 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_6 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_7 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_8 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_9 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_10 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_11 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_12 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_13 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_15 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_16 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_17 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_18 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_19 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_20 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_22 | 0.0 | 1.0 | 0.0 |
| NKFPMKOK_23 | 1.0 | 0.0 | 1.0 |
| NKFPMKOK_24 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NKFPMKOK_2 | 263564 | 288479 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG5535, VOG3390, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG5872, VOG2621, VOG1329, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227, VOG0796, VOG4681, VOG4972, VOG5447 |
| NKFPMKOK_3 | 302090 | 327322 | False | Siphoviridae | VOG10230, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0539, VOG0704, VOG4589, VOG0204, VOG3435, VOG4553, VOG4573, VOG4556, VOG4556, VOG4544, VOG0519, VOG0531, VOG7884, VOG0275, VOG0198, VOG6242, VOG6517 |
| NKFPMKOK_7 | 34100 | 58426 | False | Siphoviridae | VOG5534, VOG7718, VOG3390, VOG5008, VOG8610, VOG7930, VOG0275, VOG1637, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660, VOG10230, VOG8537 |
| NKFPMKOK_7 | 450166 | 478432 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG9667, VOG4602 |
| NKFPMKOK_8 | 228668 | 234187 | False | Siphoviridae | VOG4603, VOG4600, VOG1309, VOG11060, VOG0189 |
| NKFPMKOK_8 | 237986 | 265905 | False | Myoviridae | VOG10057, VOG0796, VOG10227, VOG4773, VOG4688, VOG6480, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG0979, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| NKFPMKOK_18 | 3520 | 10430 | False | Unknown | VOG1309, VOG4600, VOG4552, VOG0650, VOG11075, VOG9667, VOG8483 |
Phigaro v2.4.0 (Database: Jan. 2024 release)