Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NFKMLMMI_6 | 139438 | 140482 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.81 | 99.33 | KR610408 |
| NFKMLMMI_1 | 87560 | 88936 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NFKMLMMI_1 | 87560 | 88930 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| NFKMLMMI_6 | 137169 | 137687 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| NFKMLMMI_23 | 21930 | 24527 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
| NFKMLMMI_6 | 139832 | 140482 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | PARTIALX | 91.71 | 62.18 | WP_012116915.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NFKMLMMI_32 | 158846 | 160027 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| NFKMLMMI_32 | 158382 | 158831 | capC | 7.69e-115 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NFKMLMMI_17 | 31663 | 32799 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| NFKMLMMI_8 | 12480 | 11611 | pdxS | 6.35e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| NFKMLMMI_7 | 45934 | 46710 | CodY | 1.42e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| NFKMLMMI_41 | 31320 | 30928 | spxA1 | 4.43e-71 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| NFKMLMMI_6 | 122942 | 123139 | CspA | 2.57e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| NFKMLMMI_39 | 28112 | 27918 | CspD | 1.47e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| NFKMLMMI_39 | 28112 | 27915 | CspB | 3.05e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| NFKMLMMI_6 | 122942 | 123136 | CspR | 1.27e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NFKMLMMI_1 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_2 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_3 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_4 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_5 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_6 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_7 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_8 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_9 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_10 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_11 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_12 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_13 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_14 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_15 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_16 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_17 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_18 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_19 | 1.0 | 0.0 | 1.0 |
| NFKMLMMI_20 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_21 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_23 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_24 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_25 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_26 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_27 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_28 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_29 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_30 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_31 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_32 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_33 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_34 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_35 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_36 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_37 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_38 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_39 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_40 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_41 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_42 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_43 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_44 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_45 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_46 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_47 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_48 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_49 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_50 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_51 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_52 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_53 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_54 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_55 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_56 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_57 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_58 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_59 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_60 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_61 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_62 | 1.0 | 0.0 | 1.0 |
| NFKMLMMI_63 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_64 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_65 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_66 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_67 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_68 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_69 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_70 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_71 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_72 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_73 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_74 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_75 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_76 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_77 | 1.0 | 0.0 | 1.0 |
| NFKMLMMI_78 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_79 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_80 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_81 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_83 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_84 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_85 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_86 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_87 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_88 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_89 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_90 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_92 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_93 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_96 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_97 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_100 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_103 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_104 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_105 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_106 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_107 | 0.0 | 1.0 | 0.0 |
| NFKMLMMI_111 | 1.0 | 0.0 | 1.0 |
| NFKMLMMI_112 | 1.0 | 0.0 | 1.0 |
| NFKMLMMI_114 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NFKMLMMI_1 | 129496 | 135015 | False | Siphoviridae | VOG4603, VOG4600, VOG1309, VOG11060, VOG0189 |
| NFKMLMMI_1 | 138814 | 166733 | False | Myoviridae | VOG10057, VOG0796, VOG10227, VOG4773, VOG4688, VOG6480, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG0979, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| NFKMLMMI_7 | 113971 | 138297 | False | Siphoviridae | VOG0054, VOG8537, VOG10230, VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG1637, VOG0275, VOG7930, VOG8610, VOG5008, VOG3390, VOG7718 |
| NFKMLMMI_10 | 3083 | 28315 | False | Siphoviridae | VOG6581, VOG6517, VOG6242, VOG0198, VOG0275, VOG7884, VOG0531, VOG0519, VOG4544, VOG4556, VOG4556, VOG4573, VOG4553, VOG3435, VOG0204, VOG4589, VOG0704, VOG0539, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641 |
| NFKMLMMI_35 | 48888 | 71026 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885 |
| NFKMLMMI_42 | 5997 | 29323 | False | Siphoviridae | VOG5447, VOG4972, VOG4681, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG1329, VOG2621, VOG5872, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535 |
Phigaro v2.4.0 (Database: Jan. 2024 release)