Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 0 | 1 | 5.10 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKBLEHKO_1 | 113164 | 115089 | tet(Q) | ARO:3000191 | tet(Q) | 98.75 | 97.56 | Z21523.1 |
| AKBLEHKO_51 | 16912 | 18837 | tet(Q) | ARO:3000191 | tet(Q) | 89.55 | 97.56 | Z21523.1 |
| AKBLEHKO_71 | 1249 | 2415 | tet(X) | ARO:3000205 | tet(X) | 99.74 | 100.00 | M37699.1 |
| AKBLEHKO_71 | 233 | 1036 | ErmF | ARO:3000498 | ErmF | 98.86 | 100.38 | M17124.1 |
| AKBLEHKO_47 | 137 | 1102 | CfxA4 | ARO:3003005 | CfxA4 | 99.38 | 100.00 | AY769933.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AKBLEHKO_71 | 1249 | 2415 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.91 | 100.00 | M37699 |
| AKBLEHKO_47 | 137 | 1102 | cfxA4 | Unknown Beta-lactam | 99.79 | 100.00 | AY769933 |
| AKBLEHKO_71 | 241 | 1036 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.75 | 99.38 | M17808 |
| AKBLEHKO_1 | 113164 | 115089 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.01 | 100.00 | Z21523 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKBLEHKO_71 | 1252 | 2415 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | BLASTX | 99.74 | 100.00 | WP_008651082.1 |
| AKBLEHKO_47 | 137 | 1099 | cfxA4 | extended-spectrum class A beta-lactamase CfxA4 | BLASTX | 99.38 | 100.00 | WP_057280848.1 |
| AKBLEHKO_71 | 242 | 1033 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 99.24 | 99.25 | WP_002682030.1 |
| AKBLEHKO_1 | 113167 | 115089 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 98.75 | 100.00 | WP_002560998.1 |
| AKBLEHKO_51 | 16912 | 18834 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 90.02 | 100.00 | WP_063856407.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AKBLEHKO_33 | 51495 | 53075 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 80.64 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AKBLEHKO_72 | 1990 | 2669 | repUS2 | 682 / 681 | 98.53 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AKBLEHKO_1 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_2 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_3 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_4 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_5 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_6 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_7 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_8 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_9 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_10 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_11 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_12 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_13 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_14 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_15 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_16 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_17 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_18 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_19 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_20 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_21 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_22 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_23 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_24 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_25 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_26 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_27 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_28 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_29 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_30 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_31 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_32 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_33 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_34 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_35 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_36 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_37 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_38 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_39 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_40 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_41 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_42 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_43 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_44 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_45 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_46 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_47 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_48 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_49 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_50 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_51 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_52 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_53 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_54 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_55 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_56 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_57 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_58 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_59 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_60 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_61 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_62 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_63 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_64 | 0.0 | 1.0 | 0.0 |
| AKBLEHKO_65 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_67 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_68 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_69 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_70 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_71 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_72 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_73 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_74 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_78 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_79 | 1.0 | 0.0 | 1.0 |
| AKBLEHKO_80 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AKBLEHKO_25 | 27216 | 33725 | False | Unknown | VOG6269, |
| AKBLEHKO_25 | 43578 | 58619 | False | Siphoviridae | VOG2154, |
| AKBLEHKO_28 | 57007 | 65423 | False | Unknown | VOG4573, |
Phigaro v2.4.0 (Database: Jan. 2024 release)