Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 5 | 17.15 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IKIEMMBD_1 | 3771804 | 3772724 | mphK | ARO:3004541 | mphK | 96.70 | 100.00 | CP010314.1 |
| IKIEMMBD_1 | 3758476 | 3759909 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| IKIEMMBD_1 | 3708825 | 3709418 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| IKIEMMBD_1 | 3453091 | 3454734 | vmlR | ARO:3004476 | vmlR | 97.99 | 100.00 | NC_000964.3 |
| IKIEMMBD_1 | 3168297 | 3170867 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IKIEMMBD_1 | 2709664 | 2710002 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IKIEMMBD_1 | 2709347 | 2709664 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IKIEMMBD_1 | 1689967 | 1691136 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IKIEMMBD_1 | 1516625 | 1517827 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| IKIEMMBD_1 | 1498200 | 1499054 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IKIEMMBD_1 | 34547 | 35923 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.42 | 100.00 | D12567 |
| IKIEMMBD_1 | 1498200 | 1499052 | aadK | Streptomycin | 98.83 | 99.77 | M26879 |
| IKIEMMBD_1 | 3771804 | 3772724 | mph(K) | Spiramycin, Telithromycin | 97.29 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IKIEMMBD_1 | 34547 | 35920 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_063856081.1 |
| IKIEMMBD_1 | 1498200 | 1499051 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| IKIEMMBD_1 | 3453091 | 3454731 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.99 | 100.00 | WP_003234144.1 |
| IKIEMMBD_1 | 3771816 | 3772724 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.70 | 99.02 | WP_003246254.1 |
| IKIEMMBD_1 | 37874 | 38392 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| IKIEMMBD_1 | 1994390 | 1996987 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IKIEMMBD_1 | 945907 | 946845 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.79 | 100.00 | NP_391077 |
| IKIEMMBD_1 | 942224 | 943009 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| IKIEMMBD_1 | 524010 | 525191 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IKIEMMBD_1 | 946865 | 954001 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.65 | 100.00 | NP_391076 |
| IKIEMMBD_1 | 2050476 | 2052239 | dep/capD | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_389723 |
| IKIEMMBD_1 | 944260 | 945879 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.44 | 100.00 | NP_391078 |
| IKIEMMBD_1 | 1893321 | 1893962 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| IKIEMMBD_1 | 525206 | 525655 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| IKIEMMBD_1 | 943035 | 944231 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| IKIEMMBD_1 | 525674 | 526816 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IKIEMMBD_2 | 28078 | 26942 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IKIEMMBD_3 | 11207 | 10338 | pdxS | 1.45e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IKIEMMBD_1 | 2368242 | 2367466 | CodY | 3.15e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IKIEMMBD_1 | 689635 | 689066 | ClpP | 2.34e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IKIEMMBD_2 | 105161 | 104919 | SpoVG | 1.75e-38 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IKIEMMBD_1 | 0.0 | 1.0 | 0.0 |
| IKIEMMBD_2 | 0.0 | 1.0 | 0.0 |
| IKIEMMBD_3 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IKIEMMBD_1 | 2739811 | 2765946 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)