safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	28	44.05

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
OHKPPDOB_1	353419	354258	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
OHKPPDOB_1	1618805	1619656	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
OHKPPDOB_1	1429727	1430488	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
OHKPPDOB_1	1272103	1273026	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
OHKPPDOB_1	1272986	1273936	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
OHKPPDOB_1	1273961	1275043	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
OHKPPDOB_1	1275133	1276107	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
OHKPPDOB_1	1276113	1276592	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
OHKPPDOB_1	1276785	1277903	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
OHKPPDOB_1	1279594	1280193	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
OHKPPDOB_1	1281790	1282521	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
OHKPPDOB_1	1394370	1394963	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
OHKPPDOB_1	1606327	1608072	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
OHKPPDOB_1	1608074	1609774	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
OHKPPDOB_1	1610994	1611800	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
OHKPPDOB_1	1611800	1612741	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
OHKPPDOB_1	1612738	1613886	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
OHKPPDOB_1	1162935	1164593	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
OHKPPDOB_1	1270674	1272095	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
OHKPPDOB_1	1282522	1283976	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
OHKPPDOB_1	1609792	1610994	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
OHKPPDOB_1	1267922	1268980	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
OHKPPDOB_1	1278471	1279430	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
OHKPPDOB_1	1280330	1281079	STR_RS05250	0.0	Capsule	Immune modulation	99.87	100.00	WP_011227246
OHKPPDOB_1	1281089	1281781	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
OHKPPDOB_1	1394370	1394963	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
OHKPPDOB_1	1394370	1394963	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
OHKPPDOB_1	1608074	1609774	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
OHKPPDOB_1	1281089	1281781	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
OHKPPDOB_1	1609792	1610994	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
OHKPPDOB_1	1610994	1611800	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
OHKPPDOB_1	1611800	1612741	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
OHKPPDOB_1	1348308	1349098	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
OHKPPDOB_1	1612738	1613886	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
OHKPPDOB_1	1394963	1395832	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
OHKPPDOB_1	1281790	1282521	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
OHKPPDOB_1	1280344	1281079	STER_RS05305	0.0	Capsule	Immune modulation	98.37	99.33	WP_011681174
OHKPPDOB_1	1281790	1282521	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
OHKPPDOB_1	1606327	1608072	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
OHKPPDOB_1	1282522	1283976	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
OHKPPDOB_1	1280344	1281079	STU_RS14635	0.0	Capsule	Immune modulation	97.83	99.33	WP_011226046
OHKPPDOB_1	1617425	1618684	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
OHKPPDOB_1	1282522	1283976	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
OHKPPDOB_1	1281089	1281781	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
OHKPPDOB_1	907208	908511	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.54	WP_000022832
OHKPPDOB_1	109119	110129	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
OHKPPDOB_1	1610994	1611800	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
OHKPPDOB_1	452983	454266	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
OHKPPDOB_1	1392895	1393941	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
OHKPPDOB_1	1616730	1617428	STER_RS07095	2.92e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
OHKPPDOB_1	1278179	1278415	STR_RS05235*	6.59e-119	Capsule	Immune modulation	100.00	100.00	-
OHKPPDOB_1	1615910	1616376	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
OHKPPDOB_1	1610257	1610994	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OHKPPDOB_1	1433175	1434158	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
OHKPPDOB_1	1015592	1016665	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
OHKPPDOB_1	824973	825941	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
OHKPPDOB_1	1780993	1783296	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.84	99.22	AAK74619
OHKPPDOB_1	1520035	1521516	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
OHKPPDOB_1	1036116	1038362	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
OHKPPDOB_1	373049	374041	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
OHKPPDOB_1	336937	337908	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
OHKPPDOB_1	1667158	1666007	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
OHKPPDOB_1	1632685	1630703	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
OHKPPDOB_1	1416981	1415251	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
OHKPPDOB_1	298676	297198	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
OHKPPDOB_1	907208	908509	Eno	0.0	enolase	86.21	99.77	ABP90468
OHKPPDOB_1	283941	282049	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
OHKPPDOB_1	1041287	1039578	PGM	0.0	phosphoglucomutase	83.71	99.65	ACH87041
OHKPPDOB_1	150810	151820	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
OHKPPDOB_1	1190017	1188656	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
OHKPPDOB_1	201133	199241	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
OHKPPDOB_1	892778	893668	bglB	2.82e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
OHKPPDOB_1	1101926	1100991	SP_0676	7.14e-168	transcription facot	81.73	96.89	AAK74821
OHKPPDOB_1	1320615	1319911	covR	3.39e-130	responder protein	84.68	100.00	AAK71319
OHKPPDOB_1	1486354	1485683	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
OHKPPDOB_1	1320615	1319911	walR	2.43e-120	DNA-binding response regulator	80.85	100.00	ANC99504
OHKPPDOB_1	1045241	1045909	CiaR	4.58e-120	two-component response regulator	85.65	99.55	AAK74935
OHKPPDOB_1	1045241	1045912	ciaR	5.11e-118	two component system response regulator	83.48	99.12	AIK75708
OHKPPDOB_1	611920	612606	CovR	9.73e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
OHKPPDOB_1	374338	374733	SpxA2	3.58e-74	transcriptional regulator	90.91	100.00	ERL21461
OHKPPDOB_1	1201376	1201777	SpxA1	3.64e-66	transcriptional regulator	80.60	100.00	ERL19273
OHKPPDOB_1	454424	454903	RopE (M5005_Spy_1611)	6.75e-70	probable DNA-directed RNA polymerase subunit delta	80.00	83.77	AAK34603
OHKPPDOB_1	1780993	1783290	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.81	98.08	MYN69560
OHKPPDOB_1	186675	185656	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OHKPPDOB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found