Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMHOCMMB_4 | 461396 | 462250 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BMHOCMMB_4 | 430562 | 431764 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| BMHOCMMB_1 | 425270 | 425587 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BMHOCMMB_1 | 424932 | 425270 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| BMHOCMMB_5 | 415669 | 417312 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| BMHOCMMB_4 | 192942 | 194111 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BMHOCMMB_5 | 153920 | 154513 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| BMHOCMMB_5 | 103367 | 104806 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| BMHOCMMB_5 | 91174 | 92097 | mphK | ARO:3004541 | mphK | 98.03 | 100.33 | CP010314.1 |
| BMHOCMMB_3 | 27345 | 29915 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BMHOCMMB_4 | 461396 | 462250 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| BMHOCMMB_5 | 91174 | 92088 | mph(K) | Spiramycin, Telithromycin | 98.03 | 99.35 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMHOCMMB_5 | 415672 | 417312 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| BMHOCMMB_4 | 461399 | 462250 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BMHOCMMB_5 | 91174 | 92088 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.03 | 99.67 | WP_003246254.1 |
| BMHOCMMB_12 | 54462 | 57059 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BMHOCMMB_2 | 540788 | 541969 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BMHOCMMB_12 | 154476 | 155117 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| BMHOCMMB_2 | 539163 | 540305 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| BMHOCMMB_2 | 540324 | 540773 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BMHOCMMB_12 | 8315 | 10078 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| BMHOCMMB_2 | 112915 | 113700 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.86 | 100.00 | NP_391080 |
| BMHOCMMB_2 | 101923 | 109059 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.54 | 100.00 | NP_391076 |
| BMHOCMMB_2 | 109079 | 110017 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.51 | 100.00 | NP_391077 |
| BMHOCMMB_2 | 111693 | 112889 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.41 | 100.00 | NP_391079 |
| BMHOCMMB_2 | 110045 | 111664 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.90 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BMHOCMMB_10 | 27999 | 26863 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BMHOCMMB_6 | 11134 | 10265 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BMHOCMMB_1 | 740817 | 741593 | CodY | 7.68e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BMHOCMMB_2 | 386898 | 387467 | ClpP | 6.43e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BMHOCMMB_1 | 275275 | 275667 | spxA1 | 6.04e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BMHOCMMB_1 | 275275 | 275667 | spx | 3.60e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BMHOCMMB_11 | 34593 | 34351 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BMHOCMMB_5 | 370115 | 370312 | CspA | 1.93e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BMHOCMMB_4 | 5184 | 5378 | CspD | 2.30e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| BMHOCMMB_4 | 5184 | 5381 | CspB | 4.77e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BMHOCMMB_1 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_2 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_3 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_4 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_5 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_6 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_8 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_10 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_11 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_12 | 0.0 | 1.0 | 0.0 |
| BMHOCMMB_13 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BMHOCMMB_1 | 369080 | 395437 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| BMHOCMMB_4 | 369218 | 398820 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4585, VOG0198, VOG4058 |
Phigaro v2.4.0 (Database: Jan. 2024 release)