Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EAENIOAH_1 | 389807 | 390976 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| EAENIOAH_3 | 277813 | 278130 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EAENIOAH_3 | 277475 | 277813 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| EAENIOAH_5 | 157525 | 158118 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| EAENIOAH_13 | 137263 | 139833 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| EAENIOAH_5 | 106973 | 108412 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| EAENIOAH_5 | 94157 | 95077 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| EAENIOAH_10 | 70737 | 71936 | blt | ARO:3003006 | blt | 98.75 | 99.75 | L32599.1 |
| EAENIOAH_10 | 52803 | 53657 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| EAENIOAH_4 | 30868 | 32511 | vmlR | ARO:3004476 | vmlR | 97.07 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EAENIOAH_10 | 52803 | 53657 | aadK | Streptomycin | 98.60 | 100.00 | M26879 |
| EAENIOAH_5 | 94157 | 95077 | mph(K) | Spiramycin, Telithromycin | 97.07 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EAENIOAH_10 | 52803 | 53654 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| EAENIOAH_4 | 30868 | 32508 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.07 | 100.00 | WP_003234144.1 |
| EAENIOAH_5 | 94157 | 95074 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| EAENIOAH_6 | 36991 | 37509 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.80 | 100.00 | WP_003242546.1 |
| EAENIOAH_1 | 54677 | 57274 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EAENIOAH_14 | 33482 | 34663 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| EAENIOAH_7 | 402043 | 402981 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| EAENIOAH_1 | 8355 | 10118 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| EAENIOAH_7 | 398360 | 399145 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| EAENIOAH_1 | 156182 | 156823 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.06 | 100.00 | NP_390062 |
| EAENIOAH_14 | 31857 | 32999 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| EAENIOAH_14 | 33018 | 33467 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| EAENIOAH_7 | 403001 | 410137 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.32 | 100.00 | NP_391076 |
| EAENIOAH_7 | 400396 | 402015 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
| EAENIOAH_7 | 399171 | 400367 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.66 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EAENIOAH_9 | 27995 | 26859 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EAENIOAH_27 | 4248 | 5117 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EAENIOAH_3 | 588451 | 589227 | CodY | 7.33e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EAENIOAH_7 | 130522 | 129953 | ClpP | 3.08e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| EAENIOAH_3 | 118228 | 118620 | spxA1 | 5.03e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| EAENIOAH_3 | 118228 | 118620 | spx | 3.00e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| EAENIOAH_15 | 20495 | 20737 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| EAENIOAH_4 | 88180 | 87983 | CspA | 6.30e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| EAENIOAH_1 | 168054 | 168248 | CspD | 2.92e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| EAENIOAH_18 | 5164 | 5358 | CspB | 3.43e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EAENIOAH_1 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_2 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_3 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_4 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_5 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_6 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_7 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_8 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_9 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_10 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_11 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_12 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_13 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_14 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_15 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_16 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_17 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_18 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_19 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_20 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_22 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_23 | 1.0 | 0.0 | 1.0 |
| EAENIOAH_24 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_25 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_26 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_27 | 0.0 | 1.0 | 0.0 |
| EAENIOAH_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EAENIOAH_3 | 221231 | 247979 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)