Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 17 | 34 | 38.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DHNFHIIB_1 | 163022 | 163873 | rfbD | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_014621820 |
| DHNFHIIB_1 | 166297 | 167012 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.55 | 99.86 | WP_011681442 |
| DHNFHIIB_5 | 37683 | 38414 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011227248 |
| DHNFHIIB_5 | 37683 | 38414 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011226048 |
| DHNFHIIB_5 | 37683 | 38414 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.58 | 100.00 | WP_011681176 |
| DHNFHIIB_1 | 167358 | 168128 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.48 | 99.23 | WP_011681440 |
| DHNFHIIB_1 | 163992 | 165250 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.30 | 99.92 | WP_011681445 |
| DHNFHIIB_5 | 38415 | 39875 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.26 | 99.86 | WP_011681177 |
| DHNFHIIB_14 | 32197 | 32790 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| DHNFHIIB_14 | 32790 | 33659 | rfbA | 0.0 | Capsule | Immune modulation | 90.92 | 100.00 | WP_011681286 |
| DHNFHIIB_14 | 32197 | 32790 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| DHNFHIIB_14 | 32197 | 32790 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| DHNFHIIB_5 | 38415 | 39875 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.65 | 100.00 | WP_011226049 |
| DHNFHIIB_16 | 39678 | 41336 | fbp54 | 0.0 | FBPs | Adherence | 90.60 | 99.94 | WP_011227179 |
| DHNFHIIB_5 | 38415 | 39875 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| DHNFHIIB_5 | 36982 | 37674 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| DHNFHIIB_5 | 36982 | 37674 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| DHNFHIIB_5 | 36982 | 37674 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| DHNFHIIB_8 | 90439 | 91742 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| DHNFHIIB_1 | 72897 | 73736 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| DHNFHIIB_17 | 14387 | 15397 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.18 | 99.80 | WP_002986042 |
| DHNFHIIB_20 | 27394 | 28155 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| DHNFHIIB_14 | 30881 | 31927 | rfbB | 0.0 | Capsule | Immune modulation | 83.59 | 99.90 | WP_002263087 |
| DHNFHIIB_23 | 1099 | 2382 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.53 | 99.61 | WP_000107753 |
| DHNFHIIB_5 | 36302 | 36972 | STU_RS14635 | 1.85e-149 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011226046 |
| DHNFHIIB_5 | 36302 | 36972 | STER_RS05305 | 8.62e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011681174 |
| DHNFHIIB_5 | 36302 | 36972 | STR_RS05250 | 8.74e-138 | Capsule | Immune modulation | 80.09 | 89.20 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DHNFHIIB_20 | 30845 | 31828 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| DHNFHIIB_20 | 19258 | 18185 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| DHNFHIIB_6 | 53143 | 50837 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| DHNFHIIB_2 | 85930 | 84449 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| DHNFHIIB_8 | 2655 | 3623 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| DHNFHIIB_7 | 473 | 2719 | metE | 0.0 | methionine biosynthesis enzymes | 92.52 | 100.00 | AAK74738 |
| DHNFHIIB_21 | 231 | 1202 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| DHNFHIIB_2 | 171191 | 177415 | PrtA | 0.0 | serine protease | 91.67 | 96.74 | ABJ55268 |
| DHNFHIIB_10 | 64487 | 66217 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| DHNFHIIB_1 | 149344 | 151332 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| DHNFHIIB_4 | 78269 | 77118 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| DHNFHIIB_15 | 29264 | 27786 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| DHNFHIIB_8 | 90439 | 91740 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| DHNFHIIB_15 | 13923 | 12031 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| DHNFHIIB_7 | 5638 | 3929 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| DHNFHIIB_21 | 34668 | 35759 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| DHNFHIIB_11 | 45955 | 44945 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| DHNFHIIB_16 | 25750 | 27111 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| DHNFHIIB_8 | 76020 | 76910 | bglB | 1.21e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| DHNFHIIB_7 | 2906 | 3763 | metF | 2.66e-170 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| DHNFHIIB_7 | 44887 | 43949 | SP_0676 | 7.87e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| DHNFHIIB_2 | 152413 | 153084 | rr01 | 4.54e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| DHNFHIIB_5 | 72195 | 71491 | covR | 6.48e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| DHNFHIIB_7 | 9605 | 10276 | ciaR | 1.10e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| DHNFHIIB_7 | 9605 | 10273 | CiaR | 2.04e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| DHNFHIIB_5 | 72195 | 71491 | walR | 1.47e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| DHNFHIIB_3 | 54828 | 54142 | CovR | 1.49e-120 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| DHNFHIIB_21 | 35932 | 36327 | SpxA2 | 3.59e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| DHNFHIIB_16 | 14285 | 13884 | SpxA1 | 1.10e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| DHNFHIIB_27 | 6739 | 6326 | GrpE | 1.52e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| DHNFHIIB_3 | 31620 | 31333 | spr0479 | 4.69e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| DHNFHIIB_4 | 35276 | 35022 | spr1327 | 6.40e-44 | essential gene | 90.59 | 100.00 | AAL00131 |
| DHNFHIIB_22 | 1900 | 1637 | spr0175 | 1.71e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| DHNFHIIB_22 | 1075 | 809 | spr0177 | 3.79e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| DHNFHIIB_23 | 2549 | 3040 | RopE (M5005_Spy_1611) | 1.58e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| DHNFHIIB_6 | 53143 | 50843 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.97 | 98.08 | MYN69560 |
| DHNFHIIB_11 | 9536 | 10555 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
| DHNFHIIB_7 | 105018 | 105557 | pnuC | 1.51e-94 | NAD salvage pathway importer | 83.89 | 66.91 | AAK75931 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DHNFHIIB_1 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_2 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_3 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_4 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_5 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_6 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_7 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_8 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_9 | 1.0 | 0.0 | 1.0 |
| DHNFHIIB_10 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_11 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_12 | 1.0 | 0.0 | 1.0 |
| DHNFHIIB_13 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_14 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_15 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_16 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_17 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_18 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_19 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_20 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_21 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_22 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_23 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_24 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_25 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_26 | 1.0 | 0.0 | 1.0 |
| DHNFHIIB_27 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_28 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_29 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_30 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_31 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_32 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_33 | 1.0 | 0.0 | 1.0 |
| DHNFHIIB_34 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_35 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_36 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_39 | 1.0 | 0.0 | 1.0 |
| DHNFHIIB_40 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_42 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_43 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_44 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_45 | 0.0 | 1.0 | 0.0 |
| DHNFHIIB_46 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)