Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MBGGHGCO_56 | 174370 | 176940 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| MBGGHGCO_5 | 100787 | 101956 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MBGGHGCO_7 | 95620 | 95937 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| MBGGHGCO_7 | 95282 | 95620 | ykkC | ARO:3003063 | ykkC | 97.32 | 100.00 | AL009126.1 |
| MBGGHGCO_9 | 64059 | 65687 | vmlR | ARO:3004476 | vmlR | 98.52 | 99.09 | NC_000964.3 |
| MBGGHGCO_45 | 30747 | 31601 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| MBGGHGCO_18 | 17963 | 18883 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| MBGGHGCO_45 | 12014 | 13216 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| MBGGHGCO_18 | 4631 | 6070 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MBGGHGCO_6 | 39471 | 40847 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | X08034 |
| MBGGHGCO_45 | 30749 | 31601 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| MBGGHGCO_18 | 17963 | 18883 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MBGGHGCO_6 | 39471 | 40844 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| MBGGHGCO_9 | 64062 | 65687 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.52 | 98.90 | WP_003234144.1 |
| MBGGHGCO_18 | 17966 | 18883 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| MBGGHGCO_45 | 30750 | 31601 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| MBGGHGCO_6 | 42828 | 43346 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MBGGHGCO_4 | 10509 | 13106 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MBGGHGCO_26 | 30440 | 31621 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MBGGHGCO_26 | 32104 | 33246 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| MBGGHGCO_23 | 162613 | 163254 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| MBGGHGCO_2 | 103520 | 104305 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| MBGGHGCO_26 | 31636 | 32085 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| MBGGHGCO_4 | 60419 | 62182 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| MBGGHGCO_2 | 100650 | 102269 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.64 | 100.00 | NP_391078 |
| MBGGHGCO_2 | 92528 | 99664 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.38 | 100.00 | NP_391076 |
| MBGGHGCO_2 | 99684 | 100622 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.08 | 100.00 | NP_391077 |
| MBGGHGCO_2 | 102298 | 103494 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.00 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MBGGHGCO_17 | 28095 | 26959 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| MBGGHGCO_30 | 4391 | 5260 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MBGGHGCO_12 | 119549 | 118773 | CodY | 2.74e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MBGGHGCO_1 | 153775 | 153206 | ClpP | 2.06e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MBGGHGCO_13 | 40146 | 40538 | spxA1 | 5.04e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MBGGHGCO_13 | 40146 | 40538 | spx | 3.00e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MBGGHGCO_20 | 34832 | 34590 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MBGGHGCO_9 | 6787 | 6984 | CspA | 4.09e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MBGGHGCO_23 | 151380 | 151186 | CspD | 6.41e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| MBGGHGCO_23 | 151380 | 151183 | CspB | 1.33e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MBGGHGCO_35 | 2182 | 2375 | Col(SD853) | 194 / 194 | 97.94 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MBGGHGCO_1 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_2 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_3 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_4 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_5 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_6 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_7 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_8 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_9 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_10 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_11 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_12 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_13 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_14 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_15 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_16 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_17 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_18 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_19 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_20 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_21 | 1.0 | 0.0 | 1.0 |
| MBGGHGCO_22 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_23 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_24 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_25 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_26 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_27 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_28 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_29 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_30 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_31 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_32 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_33 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_34 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_35 | 1.0 | 0.0 | 1.0 |
| MBGGHGCO_36 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_37 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_38 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_41 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_43 | 1.0 | 0.0 | 1.0 |
| MBGGHGCO_44 | 1.0 | 0.0 | 1.0 |
| MBGGHGCO_45 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_46 | 1.0 | 0.0 | 1.0 |
| MBGGHGCO_47 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_48 | 1.0 | 0.0 | 1.0 |
| MBGGHGCO_56 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_67 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_70 | 0.0 | 1.0 | 0.0 |
| MBGGHGCO_71 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MBGGHGCO_7 | 38154 | 65441 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| MBGGHGCO_14 | 23673 | 47349 | False | Siphoviridae | VOG2703, VOG1993, VOG4574, VOG0136, VOG0753, VOG5615, VOG4705, VOG3390, VOG4599, VOG4605, VOG4545, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG4555, VOG7363, VOG4564, VOG0720, VOG10227, VOG0796 |
| MBGGHGCO_14 | 53977 | 60992 | False | Unknown | VOG0189, VOG4566, VOG1309, VOG4600, VOG4552, VOG0650, VOG9667 |
| MBGGHGCO_23 | 198084 | 218467 | False | Siphoviridae | VOG10230, VOG7900, VOG9712, VOG5042, VOG3389, VOG4910, VOG6163, VOG9312, VOG4662, VOG0275, VOG7930, VOG8610 |
| MBGGHGCO_25 | 7498 | 33242 | False | Myoviridae | VOG4573, VOG2703, VOG1993, VOG4574, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG0815, VOG4553, VOG4555, VOG0720, VOG10227, VOG0796 |
Phigaro v2.4.0 (Database: Jan. 2024 release)