Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DGJHDMNE_13 | 62381 | 62698 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DGJHDMNE_13 | 62043 | 62381 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| DGJHDMNE_18 | 61079 | 62281 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| DGJHDMNE_15 | 59486 | 62056 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| DGJHDMNE_18 | 44330 | 45184 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DGJHDMNE_8 | 41013 | 41933 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| DGJHDMNE_41 | 28674 | 30320 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| DGJHDMNE_8 | 27679 | 29118 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| DGJHDMNE_49 | 978 | 1571 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DGJHDMNE_18 | 44330 | 45184 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| DGJHDMNE_8 | 41013 | 41933 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DGJHDMNE_8 | 41025 | 41933 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| DGJHDMNE_18 | 44330 | 45181 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DGJHDMNE_41 | 28674 | 30317 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| DGJHDMNE_14 | 51047 | 51565 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DGJHDMNE_1 | 253749 | 254930 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DGJHDMNE_7 | 112888 | 113673 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DGJHDMNE_1 | 255413 | 256555 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DGJHDMNE_7 | 101896 | 109032 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| DGJHDMNE_48 | 19804 | 21567 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| DGJHDMNE_7 | 109052 | 109990 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| DGJHDMNE_7 | 111666 | 112862 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| DGJHDMNE_3 | 143949 | 144590 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| DGJHDMNE_1 | 254945 | 255394 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| DGJHDMNE_7 | 110018 | 111637 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DGJHDMNE_24 | 31783 | 32919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DGJHDMNE_50 | 11278 | 10409 | pdxS | 1.96e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| DGJHDMNE_2 | 151565 | 152341 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DGJHDMNE_32 | 4454 | 5023 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DGJHDMNE_21 | 23391 | 23783 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DGJHDMNE_21 | 23391 | 23783 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DGJHDMNE_29 | 34758 | 34516 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DGJHDMNE_30 | 13827 | 14024 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DGJHDMNE_3 | 132719 | 132525 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DGJHDMNE_36 | 32111 | 31917 | CspB | 4.23e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DGJHDMNE_61 | 3734 | 3901 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DGJHDMNE_1 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_2 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_3 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_4 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_5 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_6 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_7 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_8 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_9 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_10 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_11 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_12 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_13 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_14 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_15 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_16 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_17 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_18 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_19 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_20 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_21 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_22 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_23 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_24 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_25 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_26 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_27 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_28 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_29 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_30 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_31 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_32 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_33 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_34 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_35 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_36 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_37 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_38 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_39 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_40 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_41 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_42 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_43 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_44 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_45 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_46 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_47 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_48 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_49 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_50 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_51 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_52 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_53 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_54 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_55 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_56 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_57 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_58 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_59 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_60 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_61 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_62 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_63 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_64 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_65 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_66 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_67 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_68 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_69 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_70 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_72 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_73 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_74 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_75 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_76 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_78 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_79 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_80 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_81 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_83 | 1.0 | 0.0 | 1.0 |
| DGJHDMNE_84 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_85 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_86 | 0.0 | 1.0 | 0.0 |
| DGJHDMNE_87 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DGJHDMNE_12 | 4637 | 15167 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| DGJHDMNE_13 | 5896 | 32579 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DGJHDMNE_31 | 92 | 26938 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
Phigaro v2.4.0 (Database: Jan. 2024 release)