Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIGJNNDJ_19 | 61079 | 62281 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| DIGJNNDJ_18 | 47819 | 49258 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| DIGJNNDJ_19 | 44330 | 45184 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DIGJNNDJ_12 | 40711 | 41049 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| DIGJNNDJ_12 | 40394 | 40711 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DIGJNNDJ_18 | 35004 | 35924 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| DIGJNNDJ_14 | 27446 | 30016 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| DIGJNNDJ_42 | 5731 | 7377 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| DIGJNNDJ_50 | 978 | 1571 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DIGJNNDJ_19 | 44330 | 45184 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| DIGJNNDJ_18 | 35004 | 35924 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIGJNNDJ_18 | 35004 | 35912 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| DIGJNNDJ_19 | 44330 | 45181 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DIGJNNDJ_42 | 5734 | 7377 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| DIGJNNDJ_13 | 51047 | 51565 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DIGJNNDJ_1 | 253749 | 254930 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DIGJNNDJ_6 | 112888 | 113673 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DIGJNNDJ_1 | 255413 | 256555 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DIGJNNDJ_6 | 101896 | 109032 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| DIGJNNDJ_49 | 19804 | 21567 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| DIGJNNDJ_6 | 109052 | 109990 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| DIGJNNDJ_6 | 111666 | 112862 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| DIGJNNDJ_3 | 143949 | 144590 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| DIGJNNDJ_1 | 254945 | 255394 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| DIGJNNDJ_6 | 110018 | 111637 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DIGJNNDJ_25 | 32051 | 33187 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DIGJNNDJ_52 | 4356 | 5225 | pdxS | 1.96e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| DIGJNNDJ_2 | 93912 | 93136 | CodY | 1.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DIGJNNDJ_34 | 45175 | 44606 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DIGJNNDJ_22 | 23391 | 23783 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DIGJNNDJ_22 | 23391 | 23783 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DIGJNNDJ_31 | 20607 | 20849 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DIGJNNDJ_32 | 40527 | 40330 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DIGJNNDJ_3 | 132719 | 132525 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DIGJNNDJ_37 | 32111 | 31917 | CspB | 4.23e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DIGJNNDJ_63 | 4689 | 4856 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DIGJNNDJ_1 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_2 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_3 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_4 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_5 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_6 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_7 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_8 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_9 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_10 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_11 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_12 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_13 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_14 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_15 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_16 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_17 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_18 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_19 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_20 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_21 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_22 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_23 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_24 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_25 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_26 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_27 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_28 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_29 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_30 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_31 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_32 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_33 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_34 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_35 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_36 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_37 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_38 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_39 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_40 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_41 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_42 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_43 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_44 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_45 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_46 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_47 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_48 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_49 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_50 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_51 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_52 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_53 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_54 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_55 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_56 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_57 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_58 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_59 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_60 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_61 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_62 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_63 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_64 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_65 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_66 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_67 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_68 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_69 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_70 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_71 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_73 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_74 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_75 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_76 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_78 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_79 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_80 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_82 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_83 | 1.0 | 0.0 | 1.0 |
| DIGJNNDJ_84 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_85 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_86 | 0.0 | 1.0 | 0.0 |
| DIGJNNDJ_87 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DIGJNNDJ_11 | 92652 | 103182 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587 |
| DIGJNNDJ_12 | 70513 | 97196 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)