Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BDADAOKO_1 | 2680288 | 2681142 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| BDADAOKO_1 | 2663491 | 2664693 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| BDADAOKO_1 | 2433375 | 2434544 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| BDADAOKO_1 | 1360845 | 1361162 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BDADAOKO_1 | 1360507 | 1360845 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| BDADAOKO_1 | 899655 | 902225 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BDADAOKO_1 | 582995 | 584638 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| BDADAOKO_1 | 330241 | 330834 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| BDADAOKO_1 | 279688 | 281121 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| BDADAOKO_1 | 266872 | 267792 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BDADAOKO_1 | 4130783 | 4132159 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.69 | 100.00 | X08034 |
| BDADAOKO_1 | 2680290 | 2681142 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| BDADAOKO_1 | 266872 | 267792 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BDADAOKO_1 | 582998 | 584638 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| BDADAOKO_1 | 4130786 | 4132159 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| BDADAOKO_1 | 266872 | 267789 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BDADAOKO_1 | 2680291 | 2681142 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| BDADAOKO_1 | 4128312 | 4128830 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BDADAOKO_1 | 2044004 | 2046601 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BDADAOKO_1 | 2142587 | 2143228 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| BDADAOKO_1 | 3235038 | 3235823 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.87 | 100.00 | NP_391080 |
| BDADAOKO_1 | 3653334 | 3654515 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| BDADAOKO_1 | 3651709 | 3652851 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| BDADAOKO_1 | 3652870 | 3653319 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BDADAOKO_1 | 3233816 | 3235012 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| BDADAOKO_1 | 3224046 | 3231182 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.02 | 100.00 | NP_391076 |
| BDADAOKO_1 | 3231202 | 3232140 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| BDADAOKO_1 | 1997144 | 1998907 | dep/capD | 0.0 | Capsule | Immune modulation | 98.70 | 100.00 | NP_389723 |
| BDADAOKO_1 | 3232168 | 3233787 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.79 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BDADAOKO_1 | 132399 | 133535 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BDADAOKO_1 | 18665 | 19534 | pdxS | 7.16e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BDADAOKO_1 | 1670690 | 1671466 | CodY | 3.37e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BDADAOKO_1 | 3490902 | 3491471 | ClpP | 2.50e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BDADAOKO_1 | 0.0 | 1.0 | 0.0 |
| BDADAOKO_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BDADAOKO_1 | 1303895 | 1330695 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BDADAOKO_1 | 2189731 | 2216429 | False | Siphoviridae | VOG6116, VOG0018, VOG8440, VOG4707, VOG0136, VOG4705, VOG4611, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG1886, VOG4581, VOG0052, VOG7884, VOG0713, VOG5129 |
| BDADAOKO_1 | 2228142 | 2238893 | False | Unknown | VOG4966, VOG7462, VOG0026, VOG0052, VOG5532, VOG9957, VOG6332 |
| BDADAOKO_1 | 2612429 | 2634987 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
| BDADAOKO_2 | 8375 | 22916 | False | Siphoviridae | VOG1574, VOG5292, VOG4549, VOG2725, VOG3851, VOG9332, VOG9332, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352 |
| BDADAOKO_2 | 42580 | 66224 | False | Siphoviridae | VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221 |
Phigaro v2.4.0 (Database: Jan. 2024 release)