Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 29 | 38.95 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OPFFLOFG_1 | 1335580 | 1336419 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| OPFFLOFG_1 | 746628 | 747221 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OPFFLOFG_1 | 943416 | 944372 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| OPFFLOFG_1 | 942623 | 943432 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| OPFFLOFG_1 | 746628 | 747221 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| OPFFLOFG_1 | 746628 | 747221 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| OPFFLOFG_1 | 944369 | 945517 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| OPFFLOFG_1 | 949013 | 949864 | rfbD | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_014621820 |
| OPFFLOFG_1 | 940343 | 941398 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_002948022 |
| OPFFLOFG_1 | 499808 | 501466 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| OPFFLOFG_1 | 941418 | 942623 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226326 |
| OPFFLOFG_1 | 938562 | 940346 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| OPFFLOFG_1 | 633392 | 633991 | STR_RS05245 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_196767671 |
| OPFFLOFG_1 | 747221 | 748090 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| OPFFLOFG_1 | 612213 | 613628 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.01 | 100.00 | WP_011681165 |
| OPFFLOFG_1 | 702101 | 702891 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| OPFFLOFG_1 | 945626 | 946636 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011226330 |
| OPFFLOFG_1 | 781986 | 782747 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| OPFFLOFG_1 | 636320 | 637775 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.69 | 100.00 | WP_011226049 |
| OPFFLOFG_1 | 636320 | 637775 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.56 | 100.00 | WP_041827043 |
| OPFFLOFG_1 | 632269 | 633228 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 98.23 | 100.00 | WP_041827042 |
| OPFFLOFG_1 | 946636 | 947910 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.20 | 100.00 | WP_011226331 |
| OPFFLOFG_1 | 634142 | 634877 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.83 | 99.33 | WP_011681174 |
| OPFFLOFG_1 | 634142 | 634877 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.69 | 99.33 | WP_011226046 |
| OPFFLOFG_1 | 635588 | 636319 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011681176 |
| OPFFLOFG_1 | 634128 | 634877 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.33 | 100.00 | WP_011227246 |
| OPFFLOFG_1 | 635588 | 636319 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.27 | 100.00 | WP_011226048 |
| OPFFLOFG_1 | 636320 | 637775 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.12 | 99.86 | WP_011681177 |
| OPFFLOFG_1 | 635588 | 636319 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 96.86 | 100.00 | WP_011227248 |
| OPFFLOFG_1 | 634887 | 635579 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 93.51 | 100.00 | WP_011226047 |
| OPFFLOFG_1 | 634887 | 635579 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 93.22 | 100.00 | WP_011227247 |
| OPFFLOFG_1 | 250125 | 251428 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.86 | 99.54 | WP_000022832 |
| OPFFLOFG_1 | 1233479 | 1234489 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| OPFFLOFG_1 | 634887 | 635579 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 86.29 | 100.00 | WP_011681175 |
| OPFFLOFG_1 | 1610543 | 1611826 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| OPFFLOFG_1 | 745153 | 746199 | rfbB | 0.0 | Capsule | Immune modulation | 80.33 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OPFFLOFG_1 | 785434 | 786417 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| OPFFLOFG_1 | 352091 | 353164 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| OPFFLOFG_1 | 1112808 | 1115111 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| OPFFLOFG_1 | 167920 | 168888 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| OPFFLOFG_1 | 857550 | 859031 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| OPFFLOFG_1 | 380340 | 382586 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| OPFFLOFG_1 | 1315468 | 1314470 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| OPFFLOFG_1 | 1352903 | 1351932 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| OPFFLOFG_1 | 992843 | 991692 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| OPFFLOFG_1 | 964835 | 962847 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| OPFFLOFG_1 | 769240 | 767510 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| OPFFLOFG_1 | 1391132 | 1392610 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| OPFFLOFG_1 | 250125 | 251426 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| OPFFLOFG_1 | 1406328 | 1408220 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| OPFFLOFG_1 | 385511 | 383802 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| OPFFLOFG_1 | 1572784 | 1571774 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| OPFFLOFG_1 | 528007 | 526646 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| OPFFLOFG_1 | 1522515 | 1524407 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| OPFFLOFG_1 | 235695 | 236585 | bglB | 2.85e-178 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| OPFFLOFG_1 | 446166 | 445228 | SP_0676 | 8.03e-168 | transcription facot | 81.47 | 97.20 | AAK74821 |
| OPFFLOFG_1 | 382778 | 383635 | metF | 1.47e-164 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| OPFFLOFG_1 | 674469 | 673765 | covR | 6.61e-131 | responder protein | 85.53 | 100.00 | AAK71319 |
| OPFFLOFG_1 | 838586 | 837915 | rr01 | 5.40e-125 | response regulator | 84.38 | 99.56 | CAB54566 |
| OPFFLOFG_1 | 674469 | 673765 | walR | 3.13e-121 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| OPFFLOFG_1 | 389465 | 390133 | CiaR | 3.85e-120 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| OPFFLOFG_1 | 389465 | 390136 | ciaR | 5.48e-118 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| OPFFLOFG_1 | 1759444 | 1760130 | CovR | 3.92e-117 | serves to repress GAS virulence factor-encoding genes | 80.35 | 100.00 | ACE75886 |
| OPFFLOFG_1 | 1314194 | 1313820 | SpxA2 | 1.13e-69 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| OPFFLOFG_1 | 539365 | 539766 | SpxA1 | 3.68e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| OPFFLOFG_1 | 1611984 | 1612484 | RopE (M5005_Spy_1611) | 4.81e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| OPFFLOFG_1 | 1112808 | 1115105 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| OPFFLOFG_1 | 1536918 | 1537937 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OPFFLOFG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)