Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FNPFPPAG_1 | 2627484 | 2628338 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| FNPFPPAG_1 | 2608754 | 2609956 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| FNPFPPAG_1 | 2414940 | 2416109 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FNPFPPAG_1 | 1344026 | 1344343 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FNPFPPAG_1 | 1343688 | 1344026 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FNPFPPAG_1 | 883707 | 886277 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| FNPFPPAG_1 | 585722 | 587365 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| FNPFPPAG_1 | 338841 | 339434 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| FNPFPPAG_1 | 288320 | 289753 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| FNPFPPAG_1 | 275504 | 276424 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FNPFPPAG_1 | 2627484 | 2628338 | aadK | Streptomycin | 99.30 | 100.00 | M26879 |
| FNPFPPAG_1 | 4082850 | 4084226 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | X08034 |
| FNPFPPAG_1 | 275504 | 276424 | mph(K) | Spiramycin, Telithromycin | 97.83 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FNPFPPAG_1 | 2627487 | 2628338 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| FNPFPPAG_1 | 4082853 | 4084226 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| FNPFPPAG_1 | 585725 | 587365 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| FNPFPPAG_1 | 275504 | 276421 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| FNPFPPAG_1 | 4080379 | 4080897 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| FNPFPPAG_1 | 2070328 | 2072925 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FNPFPPAG_1 | 2019871 | 2021634 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| FNPFPPAG_1 | 2190615 | 2191256 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| FNPFPPAG_1 | 3182305 | 3183501 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.50 | 100.00 | NP_391079 |
| FNPFPPAG_1 | 3590481 | 3591666 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.25 | NP_391471 |
| FNPFPPAG_1 | 3183527 | 3184312 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| FNPFPPAG_1 | 3180657 | 3182276 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| FNPFPPAG_1 | 3179691 | 3180629 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| FNPFPPAG_1 | 3588856 | 3589998 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| FNPFPPAG_1 | 3590017 | 3590466 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FNPFPPAG_1 | 3172535 | 3179671 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FNPFPPAG_1 | 126539 | 127675 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FNPFPPAG_1 | 18664 | 19533 | pdxS | 3.05e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FNPFPPAG_1 | 1654028 | 1654804 | CodY | 3.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FNPFPPAG_1 | 3435986 | 3436555 | ClpP | 8.37e-98 | part of proteolytic complex | 80.53 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FNPFPPAG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FNPFPPAG_1 | 1287418 | 1314207 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| FNPFPPAG_1 | 1853247 | 1879942 | False | Siphoviridae | VOG0371, VOG0198, VOG0275, VOG10900, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG10223, VOG0204, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)