Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PFACBKAP_1 | 4307047 | 4307967 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
| PFACBKAP_1 | 4293718 | 4295151 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| PFACBKAP_1 | 4244002 | 4244595 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| PFACBKAP_1 | 3996345 | 3997988 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| PFACBKAP_1 | 3637081 | 3639651 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| PFACBKAP_1 | 3170496 | 3170834 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| PFACBKAP_1 | 3170179 | 3170496 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| PFACBKAP_1 | 2010491 | 2011660 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| PFACBKAP_1 | 1783441 | 1784643 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| PFACBKAP_1 | 1758252 | 1759106 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PFACBKAP_1 | 1758252 | 1759104 | aadK | Streptomycin | 99.30 | 99.77 | M26879 |
| PFACBKAP_1 | 4307047 | 4307967 | mph(K) | Spiramycin, Telithromycin | 99.24 | 100.00 | NC_000964 |
| PFACBKAP_1 | 190398 | 191774 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.69 | 100.00 | D12567 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PFACBKAP_1 | 4307050 | 4307967 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| PFACBKAP_1 | 3996345 | 3997985 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| PFACBKAP_1 | 190398 | 191771 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| PFACBKAP_1 | 1758252 | 1759103 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| PFACBKAP_1 | 193766 | 194284 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
| PFACBKAP_1 | 2486716 | 2489313 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PFACBKAP_1 | 688388 | 689569 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| PFACBKAP_1 | 1153248 | 1154033 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| PFACBKAP_1 | 1156931 | 1157869 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| PFACBKAP_1 | 2247948 | 2248589 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| PFACBKAP_1 | 2533321 | 2535084 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| PFACBKAP_1 | 1154059 | 1155255 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| PFACBKAP_1 | 1157889 | 1165025 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.13 | 100.00 | NP_391076 |
| PFACBKAP_1 | 689584 | 690033 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| PFACBKAP_1 | 690052 | 691194 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| PFACBKAP_1 | 1155284 | 1156903 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.27 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PFACBKAP_1 | 32834 | 31698 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| PFACBKAP_1 | 146570 | 145701 | pdxS | 3.32e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| PFACBKAP_1 | 2860533 | 2859757 | CodY | 3.63e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PFACBKAP_1 | 851314 | 850745 | ClpP | 2.69e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PFACBKAP_1 | 0.0 | 1.0 | 0.0 |
| PFACBKAP_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PFACBKAP_1 | 1056499 | 1085899 | False | Siphoviridae | VOG4552, VOG11060, VOG9600, VOG0025, VOG1884, VOG0198, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG3390, VOG4705, VOG0641, VOG4574 |
| PFACBKAP_1 | 1267406 | 1289917 | False | Siphoviridae | VOG0198, VOG8642, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4605, VOG4599, VOG5535, VOG4728, VOG0753 |
| PFACBKAP_1 | 1815854 | 1833416 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| PFACBKAP_1 | 2199219 | 2216446 | False | Siphoviridae | VOG3390, VOG4705, VOG8610, VOG3699, VOG0221, VOG7734, VOG0720, VOG4555, VOG4572, VOG4713, VOG9219, VOG4910 |
| PFACBKAP_1 | 2286362 | 2292964 | False | Siphoviridae | VOG4705, VOG3390, VOG4626, VOG9712, VOG5042 |
| PFACBKAP_1 | 2364944 | 2371836 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025, VOG4551 |
| PFACBKAP_1 | 3200030 | 3226645 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| PFACBKAP_1 | 3909740 | 3934361 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG4728, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG7363, VOG4564, VOG0720, VOG10227, VOG0796 |
| PFACBKAP_1 | 3940824 | 3952355 | False | Siphoviridae | VOG0189, VOG4566, VOG1309, VOG4600, VOG4552, VOG0650, VOG9667, VOG9667, VOG3304, VOG4602, VOG0275 |
| PFACBKAP_1 | 4427983 | 4453239 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG1581, VOG0749, VOG4685, VOG4688, VOG4773 |
| PFACBKAP_1 | 4465786 | 4471543 | False | Unknown | VOG1292, VOG1309, VOG0083, VOG1878, VOG3549 |
| PFACBKAP_2 | 7296 | 29930 | False | Siphoviridae | VOG0221, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG4605, VOG4599, VOG9244, VOG10944, VOG1024, VOG3616 |
| PFACBKAP_2 | 48114 | 67304 | False | Siphoviridae | VOG0052, VOG11080, VOG2352, VOG0397, VOG1198, VOG4561, VOG4714, VOG2352, VOG3851, VOG3531, VOG2725, VOG4549, VOG5292, VOG1574, VOG0918, VOG4551 |
Phigaro v2.4.0 (Database: Jan. 2024 release)