Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHONCCNG_26 | 630309 | 630647 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MHONCCNG_26 | 629992 | 630309 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MHONCCNG_27 | 523658 | 524860 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| MHONCCNG_27 | 505345 | 506199 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| MHONCCNG_6 | 353607 | 354776 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MHONCCNG_5 | 183608 | 185251 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| MHONCCNG_25 | 123367 | 125937 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MHONCCNG_8 | 108006 | 108926 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| MHONCCNG_8 | 94673 | 96112 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| MHONCCNG_8 | 45041 | 45634 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MHONCCNG_11 | 322380 | 323756 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| MHONCCNG_27 | 505345 | 506197 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| MHONCCNG_8 | 108006 | 108926 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHONCCNG_11 | 322383 | 323756 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| MHONCCNG_5 | 183611 | 185251 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| MHONCCNG_27 | 505345 | 506196 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| MHONCCNG_8 | 108009 | 108926 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| MHONCCNG_11 | 319909 | 320427 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MHONCCNG_6 | 56015 | 58612 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MHONCCNG_6 | 152161 | 152802 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| MHONCCNG_24 | 30009 | 30947 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| MHONCCNG_24 | 474632 | 475813 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MHONCCNG_24 | 30975 | 32594 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| MHONCCNG_24 | 33845 | 34630 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| MHONCCNG_24 | 473007 | 474149 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| MHONCCNG_24 | 32623 | 33819 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| MHONCCNG_6 | 9084 | 10847 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| MHONCCNG_24 | 22853 | 29989 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| MHONCCNG_24 | 474168 | 474617 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MHONCCNG_9 | 27942 | 26806 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MHONCCNG_14 | 11190 | 10321 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MHONCCNG_26 | 320447 | 319671 | CodY | 5.45e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MHONCCNG_24 | 333215 | 333784 | ClpP | 3.83e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MHONCCNG_2 | 109894 | 109502 | spxA1 | 1.63e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MHONCCNG_2 | 109894 | 109502 | spx | 9.71e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MHONCCNG_7 | 34595 | 34353 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MHONCCNG_5 | 136877 | 137074 | CspA | 9.26e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MHONCCNG_6 | 164764 | 164958 | CspD | 3.46e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| MHONCCNG_4 | 33099 | 32905 | CspB | 1.20e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MHONCCNG_1 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_2 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_3 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_4 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_5 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_6 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_7 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_8 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_9 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_11 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_12 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_13 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_14 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_15 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_17 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_22 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_23 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_24 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_25 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_26 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_27 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_28 | 0.0 | 1.0 | 0.0 |
| MHONCCNG_29 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MHONCCNG_2 | 5460 | 15381 | False | Siphoviridae | VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| MHONCCNG_3 | 122556 | 145596 | False | Siphoviridae | VOG2703, VOG4574, VOG0136, VOG0753, VOG4728, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG1329, VOG4555, VOG4564, VOG0720, VOG10227 |
| MHONCCNG_6 | 531784 | 556032 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796 |
| MHONCCNG_24 | 99778 | 133785 | False | Siphoviridae | VOG4574, VOG0641, VOG5008, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG9091, VOG1884, VOG0025, VOG9600, VOG4566, VOG4552, VOG1638, VOG0787, VOG4556, VOG0181, VOG5353, VOG9667 |
| MHONCCNG_26 | 41912 | 47467 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322, VOG6211 |
| MHONCCNG_26 | 95522 | 123338 | False | Siphoviridae | VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG3390, VOG4705, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660 |
| MHONCCNG_26 | 660453 | 667548 | False | Myoviridae | VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894 |
| MHONCCNG_27 | 321850 | 351010 | False | Siphoviridae | VOG0044, VOG6517, VOG0198, VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641, VOG4574 |
| MHONCCNG_29 | 4251 | 10216 | False | Unknown | VOG3549, VOG1878, VOG0083, VOG1309, VOG1292 |
| MHONCCNG_29 | 23383 | 48044 | False | Myoviridae | VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG1581, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG0906, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)