Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FKLKJFKK_3 | 523414 | 524616 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| FKLKJFKK_3 | 505101 | 505955 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| FKLKJFKK_4 | 428088 | 428405 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FKLKJFKK_4 | 427750 | 428088 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FKLKJFKK_6 | 323416 | 324336 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| FKLKJFKK_6 | 310083 | 311522 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| FKLKJFKK_6 | 260451 | 261044 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| FKLKJFKK_5 | 207284 | 208453 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FKLKJFKK_6 | 29035 | 30678 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| FKLKJFKK_15 | 27188 | 29758 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FKLKJFKK_1 | 126677 | 128053 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| FKLKJFKK_3 | 505101 | 505953 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| FKLKJFKK_6 | 323416 | 324336 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FKLKJFKK_1 | 126680 | 128053 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| FKLKJFKK_6 | 29035 | 30675 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| FKLKJFKK_3 | 505101 | 505952 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| FKLKJFKK_6 | 323419 | 324336 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| FKLKJFKK_1 | 124206 | 124724 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| FKLKJFKK_5 | 503448 | 506045 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FKLKJFKK_5 | 409258 | 409899 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| FKLKJFKK_2 | 726469 | 727407 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| FKLKJFKK_2 | 281603 | 282784 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FKLKJFKK_2 | 724822 | 726441 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| FKLKJFKK_2 | 722786 | 723571 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| FKLKJFKK_2 | 283267 | 284409 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| FKLKJFKK_2 | 723597 | 724793 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| FKLKJFKK_5 | 551213 | 552976 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| FKLKJFKK_2 | 727427 | 734563 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| FKLKJFKK_2 | 282799 | 283248 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FKLKJFKK_14 | 31724 | 32860 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FKLKJFKK_11 | 11183 | 10314 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FKLKJFKK_4 | 737950 | 738726 | CodY | 8.58e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FKLKJFKK_2 | 424201 | 423632 | ClpP | 4.55e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FKLKJFKK_4 | 276347 | 276739 | spxA1 | 6.75e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FKLKJFKK_4 | 276347 | 276739 | spx | 4.02e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FKLKJFKK_10 | 20678 | 20920 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FKLKJFKK_6 | 77409 | 77212 | CspA | 1.40e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FKLKJFKK_5 | 397296 | 397102 | CspD | 3.46e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FKLKJFKK_1 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_2 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_3 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_4 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_5 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_6 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_7 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_8 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_9 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_10 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_11 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_12 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_13 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_14 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_15 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_16 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_17 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_18 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_20 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_21 | 0.0 | 1.0 | 0.0 |
| FKLKJFKK_22 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FKLKJFKK_2 | 623631 | 657638 | False | Siphoviridae | VOG4602, VOG9667, VOG5353, VOG0181, VOG4556, VOG0787, VOG1638, VOG4552, VOG4566, VOG9600, VOG0025, VOG1884, VOG9091, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641, VOG4574 |
| FKLKJFKK_3 | 321606 | 350766 | False | Siphoviridae | VOG0044, VOG6517, VOG0198, VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641, VOG4574 |
| FKLKJFKK_4 | 370860 | 397944 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| FKLKJFKK_4 | 935059 | 962875 | False | Siphoviridae | VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG5008, VOG4705, VOG3390, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867 |
| FKLKJFKK_4 | 1011323 | 1016485 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025 |
| FKLKJFKK_5 | 6131 | 30379 | False | Myoviridae | VOG7060, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| FKLKJFKK_7 | 7115 | 31776 | False | Myoviridae | VOG4705, VOG7718, VOG0906, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG1581, VOG0749, VOG4685, VOG4688, VOG4773 |
| FKLKJFKK_7 | 44943 | 50385 | False | Unknown | VOG11060, VOG1292, VOG1309, VOG0083 |
| FKLKJFKK_9 | 7929 | 30969 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG1329, VOG1329, VOG3480, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535, VOG4728, VOG0753, VOG0136, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)