Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AJLADPGO_5 | 630327 | 630665 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| AJLADPGO_5 | 630010 | 630327 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| AJLADPGO_7 | 523429 | 524631 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| AJLADPGO_7 | 505116 | 505970 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| AJLADPGO_4 | 352765 | 353934 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| AJLADPGO_8 | 118767 | 119687 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| AJLADPGO_10 | 105456 | 108026 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| AJLADPGO_8 | 105434 | 106873 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| AJLADPGO_8 | 55802 | 56395 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| AJLADPGO_18 | 2481 | 4124 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AJLADPGO_6 | 201936 | 203312 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| AJLADPGO_7 | 505116 | 505968 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| AJLADPGO_8 | 118767 | 119687 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AJLADPGO_6 | 201939 | 203312 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| AJLADPGO_18 | 2484 | 4124 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| AJLADPGO_7 | 505116 | 505967 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| AJLADPGO_8 | 118770 | 119687 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| AJLADPGO_6 | 199465 | 199983 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| AJLADPGO_4 | 55173 | 57770 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AJLADPGO_4 | 151319 | 151960 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| AJLADPGO_2 | 559710 | 560648 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| AJLADPGO_2 | 114844 | 116025 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| AJLADPGO_2 | 558063 | 559682 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| AJLADPGO_2 | 556027 | 556812 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| AJLADPGO_2 | 116508 | 117650 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| AJLADPGO_2 | 556838 | 558034 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| AJLADPGO_4 | 8242 | 10005 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| AJLADPGO_2 | 560668 | 567804 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| AJLADPGO_2 | 116040 | 116489 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AJLADPGO_11 | 27942 | 26806 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| AJLADPGO_24 | 4253 | 5122 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| AJLADPGO_5 | 320465 | 319689 | CodY | 5.45e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AJLADPGO_2 | 257442 | 256873 | ClpP | 4.03e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| AJLADPGO_1 | 276347 | 276739 | spxA1 | 2.28e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| AJLADPGO_1 | 276347 | 276739 | spx | 1.36e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| AJLADPGO_19 | 20508 | 20750 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| AJLADPGO_15 | 44508 | 44311 | CspA | 5.86e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| AJLADPGO_4 | 163922 | 164116 | CspD | 3.45e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| AJLADPGO_1 | 33099 | 32905 | CspB | 3.28e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AJLADPGO_1 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_2 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_3 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_4 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_5 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_6 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_7 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_8 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_9 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_10 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_11 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_12 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_13 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_14 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_15 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_17 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_18 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_19 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_20 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_21 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_22 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_23 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_24 | 0.0 | 1.0 | 0.0 |
| AJLADPGO_25 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AJLADPGO_2 | 456872 | 490879 | False | Siphoviridae | VOG4602, VOG9667, VOG5353, VOG0181, VOG4556, VOG0787, VOG1638, VOG4552, VOG4566, VOG9600, VOG0025, VOG1884, VOG9091, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641, VOG4574 |
| AJLADPGO_4 | 530942 | 555190 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796 |
| AJLADPGO_5 | 41930 | 47485 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322, VOG6211 |
| AJLADPGO_5 | 95540 | 123356 | False | Siphoviridae | VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG3390, VOG4705, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660 |
| AJLADPGO_5 | 660471 | 667566 | False | Myoviridae | VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894 |
| AJLADPGO_7 | 321621 | 350781 | False | Siphoviridae | VOG0044, VOG6517, VOG0198, VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641, VOG4574 |
| AJLADPGO_9 | 6656 | 31317 | False | Myoviridae | VOG4705, VOG7718, VOG0906, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG1581, VOG0749, VOG4685, VOG4688, VOG4773 |
| AJLADPGO_9 | 45265 | 49926 | False | Unknown | VOG1292, VOG1309, VOG0083 |
| AJLADPGO_12 | 140457 | 163497 | False | Siphoviridae | VOG2703, VOG4574, VOG0136, VOG0753, VOG4728, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG1329, VOG4555, VOG4564, VOG0720, VOG10227 |
| AJLADPGO_13 | 13911 | 23832 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685 |
Phigaro v2.4.0 (Database: Jan. 2024 release)