Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 29 | 38.95 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GKMKNBGL_1 | 43604 | 44443 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| GKMKNBGL_1 | 1287565 | 1288416 | rfbD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_014621820 |
| GKMKNBGL_1 | 1071123 | 1071716 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| GKMKNBGL_1 | 1276834 | 1278534 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_041827077 |
| GKMKNBGL_1 | 1279754 | 1280560 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011227429 |
| GKMKNBGL_1 | 1280560 | 1281501 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011227430 |
| GKMKNBGL_1 | 1281498 | 1282646 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011227431 |
| GKMKNBGL_1 | 1275087 | 1276832 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_011227426 |
| GKMKNBGL_1 | 1071123 | 1071716 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GKMKNBGL_1 | 1278552 | 1279754 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011227428 |
| GKMKNBGL_1 | 1071123 | 1071716 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GKMKNBGL_1 | 837252 | 838910 | fbp54 | 0.0 | FBPs | Adherence | 99.82 | 100.00 | WP_011227179 |
| GKMKNBGL_1 | 959711 | 960442 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.73 | 100.00 | WP_011227248 |
| GKMKNBGL_1 | 1276834 | 1278534 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 99.71 | 100.00 | WP_041827753 |
| GKMKNBGL_1 | 1278552 | 1279754 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681436 |
| GKMKNBGL_1 | 1279754 | 1280560 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011681437 |
| GKMKNBGL_1 | 1280560 | 1281501 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | WP_011681438 |
| GKMKNBGL_1 | 1026310 | 1027100 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.24 | 100.00 | - |
| GKMKNBGL_1 | 1281498 | 1282646 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.22 | 100.00 | WP_011681439 |
| GKMKNBGL_1 | 1106482 | 1107243 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| GKMKNBGL_1 | 1071716 | 1072585 | rfbA | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681286 |
| GKMKNBGL_1 | 958260 | 959000 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681174 |
| GKMKNBGL_1 | 959711 | 960442 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011681176 |
| GKMKNBGL_1 | 959711 | 960442 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011226048 |
| GKMKNBGL_1 | 960443 | 961903 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.43 | 100.00 | WP_011681177 |
| GKMKNBGL_1 | 1275087 | 1276832 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 98.17 | 100.00 | WP_011681434 |
| GKMKNBGL_1 | 958265 | 959000 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.83 | 98.13 | WP_011227246 |
| GKMKNBGL_1 | 958260 | 959000 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| GKMKNBGL_1 | 1286185 | 1287444 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 97.70 | 100.00 | WP_011681445 |
| GKMKNBGL_1 | 956836 | 958203 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 97.00 | 100.00 | WP_011226045 |
| GKMKNBGL_1 | 960443 | 961903 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.99 | 100.21 | WP_011226049 |
| GKMKNBGL_1 | 960443 | 961903 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_041827043 |
| GKMKNBGL_1 | 955158 | 955607 | STU_RS14620 | 0.0 | Capsule | Immune modulation | 96.22 | 100.00 | WP_011226042 |
| GKMKNBGL_1 | 959010 | 959702 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.24 | 100.00 | WP_011227247 |
| GKMKNBGL_1 | 959010 | 959702 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_011226047 |
| GKMKNBGL_1 | 956840 | 958203 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.65 | 99.71 | WP_011681173 |
| GKMKNBGL_1 | 955616 | 956607 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 91.19 | 99.70 | - |
| GKMKNBGL_1 | 959010 | 959702 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.62 | 100.00 | WP_011681175 |
| GKMKNBGL_1 | 598328 | 599631 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| GKMKNBGL_1 | 1567872 | 1568882 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| GKMKNBGL_1 | 1279754 | 1280560 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.90 | 100.00 | WP_012000440 |
| GKMKNBGL_1 | 1069648 | 1070694 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| GKMKNBGL_1 | 147754 | 149037 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.50 | 99.77 | WP_000107753 |
| GKMKNBGL_1 | 1285490 | 1286188 | STER_RS07095 | 2.90e-152 | Capsule | Immune modulation | 81.15 | 70.35 | WP_011681444 |
| GKMKNBGL_1 | 1284670 | 1285136 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.02 | 65.13 | WP_011681442 |
| GKMKNBGL_1 | 1279017 | 1279754 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 85.09 | 60.29 | WP_012000441 |
| GKMKNBGL_1 | 954851 | 955158 | STU_RS14615 | 2.38e-113 | Capsule | Immune modulation | 91.23 | 63.37 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GKMKNBGL_1 | 1109930 | 1110913 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| GKMKNBGL_1 | 701013 | 702086 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| GKMKNBGL_1 | 514366 | 515334 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| GKMKNBGL_1 | 1449650 | 1451953 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| GKMKNBGL_1 | 1192418 | 1193899 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| GKMKNBGL_1 | 721535 | 723781 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| GKMKNBGL_1 | 63233 | 64225 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| GKMKNBGL_1 | 27122 | 28093 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| GKMKNBGL_1 | 1335924 | 1334773 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| GKMKNBGL_1 | 1301452 | 1299464 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| GKMKNBGL_1 | 1093735 | 1092005 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| GKMKNBGL_1 | 1771842 | 1770364 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GKMKNBGL_1 | 598328 | 599629 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| GKMKNBGL_1 | 1757106 | 1755214 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| GKMKNBGL_1 | 726706 | 724997 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| GKMKNBGL_1 | 1610811 | 1611821 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| GKMKNBGL_1 | 863148 | 861787 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| GKMKNBGL_1 | 1662830 | 1660938 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| GKMKNBGL_1 | 583898 | 584788 | bglB | 2.80e-178 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| GKMKNBGL_1 | 776240 | 775305 | SP_0676 | 7.11e-168 | transcription facot | 81.73 | 96.89 | AAK74821 |
| GKMKNBGL_1 | 723973 | 724830 | metF | 8.32e-165 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| GKMKNBGL_1 | 998612 | 997908 | covR | 3.37e-130 | responder protein | 84.68 | 100.00 | AAK71319 |
| GKMKNBGL_1 | 1163117 | 1162446 | rr01 | 2.84e-125 | response regulator | 84.38 | 99.56 | CAB54566 |
| GKMKNBGL_1 | 998612 | 997908 | walR | 2.42e-120 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| GKMKNBGL_1 | 297911 | 298597 | CovR | 9.68e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| GKMKNBGL_1 | 730659 | 731309 | CiaR | 1.33e-116 | two-component response regulator | 86.17 | 96.88 | AAK74935 |
| GKMKNBGL_1 | 730659 | 731309 | ciaR | 6.86e-114 | two component system response regulator | 83.87 | 96.02 | AIK75708 |
| GKMKNBGL_1 | 64522 | 64917 | SpxA2 | 3.56e-74 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| GKMKNBGL_1 | 874505 | 874906 | SpxA1 | 3.62e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| GKMKNBGL_1 | 149195 | 149695 | RopE (M5005_Spy_1611) | 9.50e-75 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| GKMKNBGL_1 | 1449650 | 1451947 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| GKMKNBGL_1 | 1648369 | 1647350 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GKMKNBGL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)