safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	29	44.69

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
BIJHBNNN_1	43663	44502	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
BIJHBNNN_1	1313760	1314611	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
BIJHBNNN_1	1124685	1125446	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
BIJHBNNN_1	967062	967985	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
BIJHBNNN_1	967945	968895	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
BIJHBNNN_1	968920	970002	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
BIJHBNNN_1	970092	971066	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
BIJHBNNN_1	971072	971551	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
BIJHBNNN_1	971744	972862	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
BIJHBNNN_1	974553	975152	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
BIJHBNNN_1	975289	976038	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
BIJHBNNN_1	976749	977480	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
BIJHBNNN_1	1089328	1089921	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
BIJHBNNN_1	1301282	1303027	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
BIJHBNNN_1	1303029	1304729	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
BIJHBNNN_1	1305949	1306755	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
BIJHBNNN_1	1306755	1307696	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
BIJHBNNN_1	1307693	1308841	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
BIJHBNNN_1	857901	859559	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
BIJHBNNN_1	965633	967054	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
BIJHBNNN_1	977481	978935	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
BIJHBNNN_1	1304747	1305949	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
BIJHBNNN_1	962881	963939	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
BIJHBNNN_1	973430	974389	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
BIJHBNNN_1	1089328	1089921	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
BIJHBNNN_1	1089328	1089921	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
BIJHBNNN_1	1303029	1304729	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
BIJHBNNN_1	976048	976740	STR_RS05255	0.0	Capsule	Immune modulation	99.71	100.00	WP_011227247
BIJHBNNN_1	1304747	1305949	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
BIJHBNNN_1	1305949	1306755	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
BIJHBNNN_1	1306755	1307696	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
BIJHBNNN_1	976048	976740	STU_RS14640	0.0	Capsule	Immune modulation	99.42	100.00	WP_011226047
BIJHBNNN_1	1043272	1044062	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
BIJHBNNN_1	1307693	1308841	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
BIJHBNNN_1	1089921	1090790	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
BIJHBNNN_1	976749	977480	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
BIJHBNNN_1	975303	976038	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
BIJHBNNN_1	976749	977480	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
BIJHBNNN_1	1301282	1303027	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
BIJHBNNN_1	977481	978935	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
BIJHBNNN_1	975303	976038	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
BIJHBNNN_1	1312380	1313639	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
BIJHBNNN_1	977481	978935	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
BIJHBNNN_1	976048	976740	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
BIJHBNNN_1	603260	604563	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.54	WP_000022832
BIJHBNNN_1	1595466	1596476	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
BIJHBNNN_1	1305949	1306755	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
BIJHBNNN_1	149037	150320	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
BIJHBNNN_1	1087853	1088899	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
BIJHBNNN_1	1311685	1312383	STER_RS07095	2.93e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
BIJHBNNN_1	973138	973374	STR_RS05235*	6.61e-119	Capsule	Immune modulation	100.00	100.00	-
BIJHBNNN_1	1310865	1311331	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
BIJHBNNN_1	1305212	1305949	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BIJHBNNN_1	1128133	1129116	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
BIJHBNNN_1	711796	712869	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
BIJHBNNN_1	520999	521967	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
BIJHBNNN_1	1475945	1478248	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
BIJHBNNN_1	1214996	1216477	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
BIJHBNNN_1	732319	734565	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
BIJHBNNN_1	63294	64286	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
BIJHBNNN_1	27181	28152	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
BIJHBNNN_1	1362114	1360963	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
BIJHBNNN_1	1327641	1325659	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
BIJHBNNN_1	1111939	1110209	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
BIJHBNNN_1	1785042	1783564	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
BIJHBNNN_1	603260	604561	Eno	0.0	enolase	86.21	99.77	ABP90468
BIJHBNNN_1	1770306	1768414	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
BIJHBNNN_1	737490	735781	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
BIJHBNNN_1	1637160	1638170	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
BIJHBNNN_1	884981	883620	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
BIJHBNNN_1	1687490	1685598	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
BIJHBNNN_1	588830	589720	bglB	2.82e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
BIJHBNNN_1	796888	795953	SP_0676	7.16e-168	transcription facot	81.73	96.89	AAK74821
BIJHBNNN_1	734757	735614	metF	8.38e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
BIJHBNNN_1	1015573	1014869	covR	3.40e-130	responder protein	84.68	100.00	AAK71319
BIJHBNNN_1	1181315	1180644	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
BIJHBNNN_1	1015573	1014869	walR	2.43e-120	DNA-binding response regulator	80.85	100.00	ANC99504
BIJHBNNN_1	307974	308660	CovR	9.75e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
BIJHBNNN_1	741443	742093	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
BIJHBNNN_1	741443	742093	ciaR	6.91e-114	two component system response regulator	83.87	96.02	AIK75708
BIJHBNNN_1	64583	64978	SpxA2	3.59e-74	transcriptional regulator	90.91	100.00	ERL21461
BIJHBNNN_1	896339	896740	SpxA1	3.65e-66	transcriptional regulator	80.60	100.00	ERL19273
BIJHBNNN_1	150478	150978	RopE (M5005_Spy_1611)	9.57e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
BIJHBNNN_1	1475945	1478242	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
BIJHBNNN_1	1673030	1672011	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BIJHBNNN_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found