Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 26 | 34.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NHNMMLEE_1 | 1142504 | 1143373 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| NHNMMLEE_1 | 1141911 | 1142504 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| NHNMMLEE_1 | 1366372 | 1367349 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681444 |
| NHNMMLEE_1 | 1367346 | 1368605 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| NHNMMLEE_1 | 1141911 | 1142504 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NHNMMLEE_1 | 1141911 | 1142504 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| NHNMMLEE_1 | 898575 | 900233 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| NHNMMLEE_1 | 1363553 | 1364323 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 99.61 | 99.23 | WP_011681440 |
| NHNMMLEE_1 | 43629 | 44468 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| NHNMMLEE_1 | 1365386 | 1366354 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011681443 |
| NHNMMLEE_1 | 1364669 | 1365384 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 99.30 | 99.86 | WP_011681442 |
| NHNMMLEE_1 | 1016144 | 1016875 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| NHNMMLEE_1 | 1016144 | 1016875 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011226048 |
| NHNMMLEE_1 | 1177274 | 1178035 | srtA | 0.0 | Sortase A | Exoenzyme | 98.56 | 100.00 | - |
| NHNMMLEE_1 | 1016144 | 1016875 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| NHNMMLEE_1 | 1004717 | 1006132 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.38 | 100.00 | WP_011681165 |
| NHNMMLEE_1 | 1084357 | 1085150 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 100.25 | - |
| NHNMMLEE_1 | 1014693 | 1015433 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.25 | 100.00 | WP_011226046 |
| NHNMMLEE_1 | 1015443 | 1016135 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.98 | 100.00 | WP_011227247 |
| NHNMMLEE_1 | 1015443 | 1016135 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.98 | 100.00 | WP_011226047 |
| NHNMMLEE_1 | 1016876 | 1018336 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681177 |
| NHNMMLEE_1 | 1014693 | 1015433 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.84 | 100.00 | WP_011681174 |
| NHNMMLEE_1 | 1014698 | 1015433 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.28 | 98.13 | WP_011227246 |
| NHNMMLEE_1 | 1013269 | 1014636 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 97.00 | 100.00 | WP_011226045 |
| NHNMMLEE_1 | 1016876 | 1018336 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.99 | 100.21 | WP_011226049 |
| NHNMMLEE_1 | 1368724 | 1369575 | rfbD | 0.0 | Capsule | Immune modulation | 96.83 | 100.00 | WP_014621820 |
| NHNMMLEE_1 | 1016876 | 1018336 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.03 | 100.21 | WP_041827043 |
| NHNMMLEE_1 | 1013271 | 1014636 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.58 | 99.85 | WP_011681173 |
| NHNMMLEE_1 | 1015443 | 1016135 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.19 | 100.00 | WP_011681175 |
| NHNMMLEE_1 | 616328 | 617631 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| NHNMMLEE_1 | 1645263 | 1646273 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.66 | 99.90 | WP_002986042 |
| NHNMMLEE_1 | 152706 | 153989 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| NHNMMLEE_1 | 1140436 | 1141482 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| NHNMMLEE_1 | 1002349 | 1002935 | SPD_RS01775 | 1.11e-141 | Capsule | Immune modulation | 82.48 | 96.70 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NHNMMLEE_1 | 1180722 | 1181705 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| NHNMMLEE_1 | 726302 | 727375 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NHNMMLEE_1 | 1535522 | 1537825 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| NHNMMLEE_1 | 529224 | 530192 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| NHNMMLEE_1 | 1257441 | 1258922 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| NHNMMLEE_1 | 759902 | 762148 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| NHNMMLEE_1 | 63260 | 64258 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| NHNMMLEE_1 | 27145 | 28116 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| NHNMMLEE_1 | 1418692 | 1417541 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| NHNMMLEE_1 | 1382610 | 1380622 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| NHNMMLEE_1 | 1164524 | 1162794 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.22 | 100.00 | AAK34199 |
| NHNMMLEE_1 | 1848303 | 1846825 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| NHNMMLEE_1 | 616328 | 617629 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NHNMMLEE_1 | 1832117 | 1830225 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| NHNMMLEE_1 | 765073 | 763364 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| NHNMMLEE_1 | 1688245 | 1689255 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| NHNMMLEE_1 | 935431 | 934070 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| NHNMMLEE_1 | 844782 | 843844 | SP_0676 | 4.61e-167 | transcription facot | 81.15 | 97.20 | AAK74821 |
| NHNMMLEE_1 | 762340 | 763197 | metF | 1.53e-164 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NHNMMLEE_1 | 1056582 | 1055878 | covR | 2.66e-130 | responder protein | 85.11 | 100.00 | AAK71319 |
| NHNMMLEE_1 | 1232836 | 1232165 | rr01 | 2.96e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| NHNMMLEE_1 | 1056582 | 1055878 | walR | 1.58e-120 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| NHNMMLEE_1 | 308530 | 309216 | CovR | 3.10e-118 | serves to repress GAS virulence factor-encoding genes | 81.22 | 100.00 | ACE75886 |
| NHNMMLEE_1 | 769028 | 769696 | CiaR | 2.03e-117 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| NHNMMLEE_1 | 769028 | 769699 | ciaR | 2.00e-115 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| NHNMMLEE_1 | 64534 | 64908 | SpxA2 | 1.16e-69 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| NHNMMLEE_1 | 154147 | 154647 | RopE (M5005_Spy_1611) | 4.94e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| NHNMMLEE_1 | 1535522 | 1537819 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| NHNMMLEE_1 | 1724106 | 1723087 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NHNMMLEE_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)