safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	28	29	40.31

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
FELANKNB_1	1110781	1111650	rfbA	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681286
FELANKNB_1	1110188	1110781	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
FELANKNB_1	1323694	1324500	STER_RS07060	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681437
FELANKNB_1	1327827	1328543	STER_RS07085	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681442
FELANKNB_1	1325438	1326586	STER_RS07070	0.0	Capsule	Immune modulation	99.91	100.00	WP_011681439
FELANKNB_1	1328544	1329512	STER_RS07090	0.0	Capsule	Immune modulation	99.90	100.00	WP_011681443
FELANKNB_1	1322492	1323694	STER_RS07055	0.0	Capsule	Immune modulation	99.83	100.00	WP_011681436
FELANKNB_1	1110188	1110781	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
FELANKNB_1	1319027	1320772	STR_RS06885	0.0	Capsule	Immune modulation	99.83	100.00	WP_011227426
FELANKNB_1	1320774	1322474	STER_RS07050	0.0	Capsule	Immune modulation	99.82	100.00	WP_041827753
FELANKNB_1	1324500	1325441	STR_RS06905	0.0	Capsule	Immune modulation	99.79	100.00	WP_011227430
FELANKNB_1	1324500	1325441	STER_RS07065	0.0	Capsule	Immune modulation	99.68	100.00	WP_011681438
FELANKNB_1	1110188	1110781	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
FELANKNB_1	1320774	1322474	STR_RS06890	0.0	Capsule	Immune modulation	99.65	100.00	WP_041827077
FELANKNB_1	879504	881162	fbp54	0.0	FBPs	Adherence	99.64	100.00	WP_011227179
FELANKNB_1	1322492	1323694	STR_RS06895	0.0	Capsule	Immune modulation	99.58	100.00	WP_011227428
FELANKNB_1	43685	44524	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
FELANKNB_1	1323694	1324500	STR_RS06900	0.0	Capsule	Immune modulation	99.50	100.00	WP_011227429
FELANKNB_1	1329530	1330507	STER_RS07095	0.0	Capsule	Immune modulation	99.39	100.00	WP_011681444
FELANKNB_1	1325438	1326586	STR_RS06910	0.0	Capsule	Immune modulation	99.30	100.00	WP_011227431
FELANKNB_1	986991	987722	STU_RS14645	0.0	Capsule	Immune modulation	99.04	100.00	WP_011226048
FELANKNB_1	986991	987722	STR_RS05260	0.0	Capsule	Immune modulation	98.91	100.00	WP_011227248
FELANKNB_1	986991	987722	STER_RS05315	0.0	Capsule	Immune modulation	98.77	100.00	WP_011681176
FELANKNB_1	1331881	1332732	rfbD	0.0	Capsule	Immune modulation	98.47	100.00	WP_014621820
FELANKNB_1	1053854	1054647	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.36	100.25	-
FELANKNB_1	1319027	1320772	STER_RS07045	0.0	Capsule	Immune modulation	98.17	100.00	WP_011681434
FELANKNB_1	987723	989183	STER_RS05320	0.0	Capsule	Immune modulation	98.15	100.00	WP_011681177
FELANKNB_1	1146979	1147740	srtA	0.0	Sortase A	Exoenzyme	97.64	100.00	-
FELANKNB_1	1326705	1327481	STER_RS07075	0.0	Capsule	Immune modulation	97.04	100.00	WP_011681440
FELANKNB_1	987723	989183	STU_RS14650	0.0	Capsule	Immune modulation	96.99	100.21	WP_011226049
FELANKNB_1	1330504	1331762	STER_RS07100	0.0	Capsule	Immune modulation	96.51	99.92	WP_011681445
FELANKNB_1	987723	989183	STR_RS05265	0.0	Capsule	Immune modulation	96.03	100.21	WP_041827043
FELANKNB_1	985540	986280	STER_RS05305	0.0	Capsule	Immune modulation	95.82	100.00	WP_011681174
FELANKNB_1	986290	986982	STU_RS14640	0.0	Capsule	Immune modulation	95.53	100.00	WP_011226047
FELANKNB_1	985540	986280	STU_RS14635	0.0	Capsule	Immune modulation	95.41	100.00	WP_011226046
FELANKNB_1	984118	985483	STER_RS05300	0.0	Capsule	Immune modulation	95.39	99.85	WP_011681173
FELANKNB_1	986290	986982	STR_RS05255	0.0	Capsule	Immune modulation	95.24	100.00	WP_011227247
FELANKNB_1	985545	986280	STR_RS05250	0.0	Capsule	Immune modulation	95.11	98.13	WP_011227246
FELANKNB_1	984116	985483	STU_RS14630	0.0	Capsule	Immune modulation	94.37	100.00	WP_011226045
FELANKNB_1	986290	986982	STER_RS05310	0.0	Capsule	Immune modulation	92.64	100.00	WP_011681175
FELANKNB_1	607083	608386	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.46	WP_000022832
FELANKNB_1	1617566	1618576	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
FELANKNB_1	1323694	1324500	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
FELANKNB_1	146621	147904	tig/ropA	0.0	Trigger factor	Stress survival	80.73	99.77	WP_000107753
FELANKNB_1	1108713	1109759	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
FELANKNB_1	974496	975079	SPD_RS01775	2.35e-143	Capsule	Immune modulation	82.73	96.20	WP_001819836
FELANKNB_1	1322957	1323694	SGO_RS05025	0.0	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FELANKNB_1	722835	723908	MntE	0.0	manganese homeostasis	94.69	99.72	ABF32167
FELANKNB_1	1150427	1151410	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.51	100.00	AAK75326
FELANKNB_1	523399	524367	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
FELANKNB_1	1495706	1498009	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
FELANKNB_1	1223693	1225174	SPD_0310	0.0	UPF0371 protein SPD_0310	92.71	100.00	ABJ54480
FELANKNB_1	743361	745607	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
FELANKNB_1	63316	64314	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
FELANKNB_1	27203	28174	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
FELANKNB_1	1381730	1380579	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
FELANKNB_1	1345909	1343921	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
FELANKNB_1	1134221	1132491	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
FELANKNB_1	1827631	1826153	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
FELANKNB_1	607083	608384	Eno	0.0	enolase	86.21	99.77	ABP90468
FELANKNB_1	1811474	1809582	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
FELANKNB_1	748531	746822	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
FELANKNB_1	1660958	1661968	pdh	0.0	pyruvate dehydrogenase	82.49	99.70	ABP91006
FELANKNB_1	908529	907168	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
FELANKNB_1	1712978	1711086	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
FELANKNB_1	592646	593536	bglB	1.32e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
FELANKNB_1	821199	820264	SP_0676	7.33e-168	transcription facot	81.73	96.89	AAK74821
FELANKNB_1	745799	746656	metF	8.58e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
FELANKNB_1	1026022	1025318	covR	2.10e-131	responder protein	85.53	100.00	AAK71319
FELANKNB_1	1204721	1204050	rr01	2.98e-124	response regulator	83.93	99.56	CAB54566
FELANKNB_1	1026022	1025318	walR	1.98e-121	DNA-binding response regulator	81.70	100.00	ANC99504
FELANKNB_1	299574	300260	CovR	1.02e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
FELANKNB_1	752485	753153	CiaR	2.00e-117	two-component response regulator	84.31	99.55	AAK74935
FELANKNB_1	752485	753156	ciaR	1.97e-115	two component system response regulator	82.14	99.12	AIK75708
FELANKNB_1	64590	64964	SpxA2	1.14e-69	transcriptional regulator	91.20	94.70	ERL21461
FELANKNB_1	919950	920351	SpxA1	3.73e-66	transcriptional regulator	80.60	100.00	ERL19273
FELANKNB_1	148062	148562	RopE (M5005_Spy_1611)	3.51e-75	probable DNA-directed RNA polymerase subunit delta	82.04	87.43	AAK34603
FELANKNB_1	1495706	1498003	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
FELANKNB_1	1698504	1697485	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FELANKNB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found