safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	27	29	39.62

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
EAJHJELC_1	1145034	1145903	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
EAJHJELC_1	1144441	1145034	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
EAJHJELC_1	1352929	1354674	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
EAJHJELC_1	1354676	1356376	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
EAJHJELC_1	1356394	1357596	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
EAJHJELC_1	1357596	1358402	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
EAJHJELC_1	1358402	1359343	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
EAJHJELC_1	1359340	1360488	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
EAJHJELC_1	1360607	1361383	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
EAJHJELC_1	1361729	1362445	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
EAJHJELC_1	1362446	1363414	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
EAJHJELC_1	1363432	1364409	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
EAJHJELC_1	1364406	1365665	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
EAJHJELC_1	1144441	1145034	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
EAJHJELC_1	1354676	1356376	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
EAJHJELC_1	1008412	1009104	STU_RS14640	Capsule	Immune modulation	99.71	100.00	WP_011226047
EAJHJELC_1	1144441	1145034	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
EAJHJELC_1	45047	45886	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
EAJHJELC_1	1357596	1358402	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
EAJHJELC_1	1358402	1359343	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
EAJHJELC_1	1356394	1357596	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
EAJHJELC_1	1359340	1360488	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
EAJHJELC_1	1008412	1009104	STR_RS05255	Capsule	Immune modulation	99.13	100.00	WP_011227247
EAJHJELC_1	1086891	1087681	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
EAJHJELC_1	894964	896622	fbp54	FBPs	Adherence	98.43	100.00	WP_011227179
EAJHJELC_1	1007662	1008402	STER_RS05305	Capsule	Immune modulation	98.38	100.00	WP_011681174
EAJHJELC_1	1007662	1008402	STU_RS14635	Capsule	Immune modulation	98.38	100.00	WP_011226046
EAJHJELC_1	1009113	1009844	STU_RS14645	Capsule	Immune modulation	98.36	100.00	WP_011226048
EAJHJELC_1	1352929	1354674	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
EAJHJELC_1	990984	992399	STER_RS05250	Capsule	Immune modulation	98.09	100.00	WP_011681165
EAJHJELC_1	1009113	1009844	STER_RS05315	Capsule	Immune modulation	98.09	100.00	WP_011681176
EAJHJELC_1	1007667	1008402	STR_RS05250	Capsule	Immune modulation	97.96	98.13	WP_011227246
EAJHJELC_1	1009845	1011305	STER_RS05320	Capsule	Immune modulation	97.74	100.00	WP_011681177
EAJHJELC_1	1009113	1009844	STR_RS05260	Capsule	Immune modulation	97.68	100.00	WP_011227248
EAJHJELC_1	1179802	1180563	srtA	Sortase A	Exoenzyme	97.64	100.00	-
EAJHJELC_1	1009845	1011305	STU_RS14650	Capsule	Immune modulation	96.85	100.21	WP_011226049
EAJHJELC_1	1365784	1366635	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
EAJHJELC_1	1009845	1011305	STR_RS05265	Capsule	Immune modulation	95.89	100.21	WP_041827043
EAJHJELC_1	1008412	1009104	STER_RS05310	Capsule	Immune modulation	89.90	100.00	WP_011681175
EAJHJELC_1	620427	621730	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
EAJHJELC_1	1661134	1662144	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
EAJHJELC_1	1357596	1358402	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
EAJHJELC_1	157053	158336	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
EAJHJELC_1	1142966	1144012	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
EAJHJELC_1	1356859	1357596	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EAJHJELC_1	1183250	1184233	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
EAJHJELC_1	725603	726676	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
EAJHJELC_1	535363	536331	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
EAJHJELC_1	1531970	1534273	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
EAJHJELC_1	1254362	1255843	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
EAJHJELC_1	758936	761182	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
EAJHJELC_1	64678	65676	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
EAJHJELC_1	28563	29534	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
EAJHJELC_1	1415303	1414152	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
EAJHJELC_1	1379674	1377686	uvrB	0.0	excinuclease ABC, B subunit	89.14	100.00	AE014259_2
EAJHJELC_1	1167052	1165322	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
EAJHJELC_1	1858003	1856525	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
EAJHJELC_1	620427	621728	Eno	0.0	enolase	86.21	99.77	ABP90468
EAJHJELC_1	1843268	1841376	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
EAJHJELC_1	764107	762398	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
EAJHJELC_1	1704128	1705138	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
EAJHJELC_1	921057	919696	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
EAJHJELC_1	1754467	1752575	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
EAJHJELC_1	605987	606877	bglB	5.30e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
EAJHJELC_1	836679	835741	SP_0676	1.13e-166	transcription facot	81.15	97.20	AAK74821
EAJHJELC_1	761374	762231	metF	1.54e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
EAJHJELC_1	1048160	1047456	covR	4.42e-131	responder protein	85.53	100.00	AAK71319
EAJHJELC_1	1235400	1234729	rr01	3.03e-124	response regulator	83.93	99.56	CAB54566
EAJHJELC_1	1048160	1047456	walR	1.86e-121	DNA-binding response regulator	82.13	100.00	ANC99504
EAJHJELC_1	310123	310809	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
EAJHJELC_1	768062	768730	CiaR	2.04e-117	two-component response regulator	84.31	99.55	AAK74935
EAJHJELC_1	768062	768733	ciaR	2.01e-115	two component system response regulator	82.14	99.12	AIK75708
EAJHJELC_1	65952	66326	SpxA2	1.16e-69	transcriptional regulator	91.20	94.70	ERL21461
EAJHJELC_1	932420	932821	SpxA1	3.79e-66	transcriptional regulator	80.60	100.00	ERL19273
EAJHJELC_1	158494	158994	RopE (M5005_Spy_1611)	4.96e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
EAJHJELC_1	1531970	1534267	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
EAJHJELC_1	1740012	1738993	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
EAJHJELC_2	1	949	rep37	949 / 948	99.47	AY250830

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EAJHJELC_1	0.0	1.0	0.0
EAJHJELC_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found