Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 28 | 29 | 40.31 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KNDBCOKN_1 | 43603 | 44442 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| KNDBCOKN_1 | 1249470 | 1250525 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.91 | 100.00 | WP_002948022 |
| KNDBCOKN_1 | 1247689 | 1249473 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226325 |
| KNDBCOKN_1 | 944728 | 945207 | STR_RS05225 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_041827041 |
| KNDBCOKN_1 | 1252543 | 1253499 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| KNDBCOKN_1 | 1251750 | 1252559 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| KNDBCOKN_1 | 1253496 | 1254644 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| KNDBCOKN_1 | 1250545 | 1251750 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.67 | 100.00 | WP_011226326 |
| KNDBCOKN_1 | 1061660 | 1062529 | rfbA | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011681286 |
| KNDBCOKN_1 | 1061067 | 1061660 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| KNDBCOKN_1 | 846235 | 847893 | fbp54 | 0.0 | FBPs | Adherence | 99.40 | 100.00 | WP_011227179 |
| KNDBCOKN_1 | 1061067 | 1061660 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226149 |
| KNDBCOKN_1 | 1061067 | 1061660 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226149 |
| KNDBCOKN_1 | 943792 | 944722 | STR_RS05220 | 0.0 | Capsule | Immune modulation | 99.25 | 95.49 | WP_011227240 |
| KNDBCOKN_1 | 938479 | 939900 | STR_RS05200 | 0.0 | Capsule | Immune modulation | 99.23 | 100.00 | WP_011227236 |
| KNDBCOKN_1 | 1096425 | 1097186 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| KNDBCOKN_1 | 1257302 | 1258576 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.90 | 100.00 | WP_011226331 |
| KNDBCOKN_1 | 1016223 | 1017013 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.86 | 99.87 | - |
| KNDBCOKN_1 | 1256340 | 1257302 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.86 | 95.25 | WP_011226330 |
| KNDBCOKN_1 | 950261 | 951715 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.69 | 100.00 | WP_011226049 |
| KNDBCOKN_1 | 950261 | 951715 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.42 | 100.00 | WP_041827043 |
| KNDBCOKN_1 | 1259679 | 1260530 | rfbD | 0.0 | Capsule | Immune modulation | 98.12 | 100.00 | WP_014621820 |
| KNDBCOKN_1 | 950261 | 951715 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.54 | 99.79 | WP_011681177 |
| KNDBCOKN_1 | 945229 | 946146 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 97.17 | 95.62 | WP_041827042 |
| KNDBCOKN_1 | 949529 | 950260 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.13 | 100.00 | WP_011226048 |
| KNDBCOKN_1 | 949529 | 950260 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.00 | 100.00 | WP_011681176 |
| KNDBCOKN_1 | 948828 | 949520 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011227247 |
| KNDBCOKN_1 | 948828 | 949520 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011226047 |
| KNDBCOKN_1 | 949529 | 950260 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 96.45 | 100.00 | WP_011227248 |
| KNDBCOKN_1 | 948081 | 948818 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.21 | 99.60 | WP_011681174 |
| KNDBCOKN_1 | 948083 | 948818 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 95.92 | 98.13 | WP_011227246 |
| KNDBCOKN_1 | 948081 | 948818 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 95.80 | 99.60 | WP_011226046 |
| KNDBCOKN_1 | 946653 | 948020 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.47 | 100.00 | WP_011226045 |
| KNDBCOKN_1 | 946655 | 948020 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.29 | 99.85 | WP_011681173 |
| KNDBCOKN_1 | 948828 | 949520 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 88.49 | 99.71 | WP_011681175 |
| KNDBCOKN_1 | 593656 | 594959 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| KNDBCOKN_1 | 1535876 | 1536886 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.02 | 99.51 | WP_002986042 |
| KNDBCOKN_1 | 137586 | 138869 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| KNDBCOKN_1 | 1059591 | 1060637 | rfbB | 0.0 | Capsule | Immune modulation | 80.42 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KNDBCOKN_1 | 1099873 | 1100856 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| KNDBCOKN_1 | 701599 | 702672 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| KNDBCOKN_1 | 510029 | 510997 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| KNDBCOKN_1 | 1415500 | 1417803 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| KNDBCOKN_1 | 1163498 | 1164979 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| KNDBCOKN_1 | 722121 | 724367 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| KNDBCOKN_1 | 27122 | 28093 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| KNDBCOKN_1 | 1301733 | 1300582 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| KNDBCOKN_1 | 1273730 | 1271742 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| KNDBCOKN_1 | 1083678 | 1081948 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| KNDBCOKN_1 | 1726388 | 1724910 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| KNDBCOKN_1 | 593656 | 594957 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| KNDBCOKN_1 | 1711192 | 1709300 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| KNDBCOKN_1 | 63239 | 64345 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| KNDBCOKN_1 | 727292 | 725583 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| KNDBCOKN_1 | 1577856 | 1578866 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| KNDBCOKN_1 | 873680 | 872319 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| KNDBCOKN_1 | 1629868 | 1627976 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| KNDBCOKN_1 | 579225 | 580115 | bglB | 1.25e-178 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| KNDBCOKN_1 | 786657 | 785719 | SP_0676 | 1.64e-167 | transcription facot | 81.47 | 97.20 | AAK74821 |
| KNDBCOKN_1 | 724559 | 725416 | metF | 8.11e-165 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| KNDBCOKN_1 | 988543 | 987839 | covR | 3.28e-130 | responder protein | 84.68 | 100.00 | AAK71319 |
| KNDBCOKN_1 | 1144535 | 1143864 | rr01 | 1.91e-125 | response regulator | 84.82 | 99.56 | CAB54566 |
| KNDBCOKN_1 | 731245 | 731913 | CiaR | 1.84e-120 | two-component response regulator | 86.10 | 99.55 | AAK74935 |
| KNDBCOKN_1 | 988543 | 987839 | walR | 2.36e-120 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| KNDBCOKN_1 | 731245 | 731916 | ciaR | 2.29e-118 | two component system response regulator | 83.93 | 99.12 | AIK75708 |
| KNDBCOKN_1 | 294048 | 294734 | CovR | 9.43e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| KNDBCOKN_1 | 64529 | 64903 | SpxA2 | 1.08e-69 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| KNDBCOKN_1 | 885038 | 885439 | SpxA1 | 3.53e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| KNDBCOKN_1 | 139027 | 139527 | RopE (M5005_Spy_1611) | 4.61e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| KNDBCOKN_1 | 1415500 | 1417797 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| KNDBCOKN_1 | 1615410 | 1614391 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KNDBCOKN_2 | 1 | 948 | rep37 | 948 / 948 | 94.30 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KNDBCOKN_1 | 0.0 | 1.0 | 0.0 |
| KNDBCOKN_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)