safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BOCMGNAC_1	1308736	1309995	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
BOCMGNAC_1	43669	44508	cbpD	Autolysin	Exoenzyme	99.88	100.00	WP_011226733
BOCMGNAC_1	955058	955537	STR_RS05225	Capsule	Immune modulation	99.79	100.00	WP_041827041
BOCMGNAC_1	1070945	1071814	rfbA	Capsule	Immune modulation	99.66	100.00	WP_011681286
BOCMGNAC_1	1306777	1307745	STER_RS07090	Capsule	Immune modulation	99.59	100.00	WP_011681443
BOCMGNAC_1	849929	851587	fbp54	FBPs	Adherence	99.52	100.00	WP_011227179
BOCMGNAC_1	1070352	1070945	STR_RS05880	Capsule	Immune modulation	99.50	100.00	WP_011226149
BOCMGNAC_1	1307763	1308739	STER_RS07095	Capsule	Immune modulation	99.49	99.90	WP_011681444
BOCMGNAC_1	1070352	1070945	STER_RS06030	Capsule	Immune modulation	99.33	100.00	WP_011226149
BOCMGNAC_1	956683	957282	STR_RS05245	Capsule	Immune modulation	99.33	100.00	WP_196767671
BOCMGNAC_1	1070352	1070945	STU_RS15260	Capsule	Immune modulation	99.33	100.00	WP_011226149
BOCMGNAC_1	954122	955052	STR_RS05220	Capsule	Immune modulation	99.25	95.49	WP_011227240
BOCMGNAC_1	948809	950230	STR_RS05200	Capsule	Immune modulation	99.23	100.00	WP_011227236
BOCMGNAC_1	1105710	1106471	srtA	Sortase A	Exoenzyme	99.08	100.00	-
BOCMGNAC_1	959618	961072	STU_RS14650	Capsule	Immune modulation	98.62	100.00	WP_011226049
BOCMGNAC_1	1025511	1026304	cshA	Cell surface hydrophobicity proteins	Adherence	98.49	100.25	-
BOCMGNAC_1	959618	961072	STR_RS05265	Capsule	Immune modulation	98.49	100.00	WP_041827043
BOCMGNAC_1	1306060	1306775	STER_RS07085	Capsule	Immune modulation	98.46	99.86	WP_011681442
BOCMGNAC_1	955559	956518	STR_RS05240	Capsule	Immune modulation	98.23	100.00	WP_041827042
BOCMGNAC_1	1304944	1305714	STER_RS07075	Capsule	Immune modulation	98.18	99.23	WP_011681440
BOCMGNAC_1	958880	959617	STER_RS05315	Capsule	Immune modulation	97.83	100.68	WP_011681176
BOCMGNAC_1	957434	958169	STER_RS05305	Capsule	Immune modulation	97.69	99.33	WP_011681174
BOCMGNAC_1	959618	961072	STER_RS05320	Capsule	Immune modulation	97.60	99.79	WP_011681177
BOCMGNAC_1	957434	958169	STU_RS14635	Capsule	Immune modulation	97.55	99.33	WP_011226046
BOCMGNAC_1	958880	959617	STU_RS14645	Capsule	Immune modulation	97.42	100.68	WP_011226048
BOCMGNAC_1	958880	959617	STR_RS05260	Capsule	Immune modulation	97.42	100.55	WP_011227248
BOCMGNAC_1	1310114	1310965	rfbD	Capsule	Immune modulation	97.30	100.00	WP_014621820
BOCMGNAC_1	957420	958169	STR_RS05250	Capsule	Immune modulation	97.20	100.00	WP_011227246
BOCMGNAC_1	958179	958871	STER_RS05310	Capsule	Immune modulation	90.04	100.00	WP_011681175
BOCMGNAC_1	958179	958871	STR_RS05255	Capsule	Immune modulation	89.47	100.00	WP_011227247
BOCMGNAC_1	958179	958871	STU_RS14640	Capsule	Immune modulation	89.47	100.00	WP_011226047
BOCMGNAC_1	609908	611211	eno	Streptococcal enolase	Exoenzyme	88.17	99.54	WP_000022832
BOCMGNAC_1	1588832	1589842	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.02	99.51	WP_002986042
BOCMGNAC_1	145244	146527	tig/ropA	Trigger factor	Stress survival	80.65	99.77	WP_000107753
BOCMGNAC_1	1068876	1069922	rfbB	Capsule	Immune modulation	80.42	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BOCMGNAC_1	1109158	1110141	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
BOCMGNAC_1	709977	711050	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
BOCMGNAC_1	527782	528750	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
BOCMGNAC_1	1470215	1472518	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
BOCMGNAC_1	730499	732745	metE	0.0	methionine biosynthesis enzymes	91.72	100.00	AAK74738
BOCMGNAC_1	1196434	1197915	SPD_0310	0.0	UPF0371 protein SPD_0310	91.70	100.00	ABJ54480
BOCMGNAC_1	63299	64297	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
BOCMGNAC_1	27185	28156	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
BOCMGNAC_1	1352350	1351199	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
BOCMGNAC_1	1324354	1322366	uvrB	0.0	excinuclease ABC, B subunit	88.99	100.00	AE014259_2
BOCMGNAC_1	1779867	1778389	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
BOCMGNAC_1	1092963	1091233	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.52	100.00	AAK34199
BOCMGNAC_1	609908	611209	Eno	0.0	enolase	86.21	99.77	ABP90468
BOCMGNAC_1	1764671	1762779	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
BOCMGNAC_1	735671	733962	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
BOCMGNAC_1	1631807	1632817	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
BOCMGNAC_1	885791	884439	mnmE	0.0	central tRNA-modifying GTPase	82.12	98.91	ABP90420
BOCMGNAC_1	1682127	1680235	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
BOCMGNAC_1	595478	596368	bglB	1.29e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
BOCMGNAC_1	795160	794222	SP_0676	7.94e-168	transcription facot	81.47	97.20	AAK74821
BOCMGNAC_1	732937	733794	metF	1.45e-164	methionine biosynthesis enzymes	86.01	99.31	AAK74739
BOCMGNAC_1	997849	997145	covR	3.39e-130	responder protein	84.68	100.00	AAK71319
BOCMGNAC_1	1168346	1167675	rr01	4.00e-126	response regulator	84.82	99.56	CAB54566
BOCMGNAC_1	997849	997145	walR	2.43e-120	DNA-binding response regulator	80.85	100.00	ANC99504
BOCMGNAC_1	301690	302376	CovR	9.91e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
BOCMGNAC_1	739625	740119	CiaR	2.06e-99	two-component response regulator	86.06	73.66	AAK74935
BOCMGNAC_1	739625	740122	ciaR	1.39e-97	two component system response regulator	83.13	73.45	AIK75708
BOCMGNAC_1	64573	64968	SpxA2	3.58e-74	transcriptional regulator	90.91	100.00	ERL21461
BOCMGNAC_1	897157	897558	SpxA1	3.64e-66	transcriptional regulator	80.60	100.00	ERL19273
BOCMGNAC_1	146685	147185	RopE (M5005_Spy_1611)	4.76e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
BOCMGNAC_1	1470215	1472512	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
BOCMGNAC_1	1667672	1666653	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BOCMGNAC_1	0.0	1.0	0.0
BOCMGNAC_2	1.0	0.0	1.0
BOCMGNAC_3	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found