safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	29	44.69

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
OOADCILA_1	43606	44445	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
OOADCILA_1	1315187	1316038	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
OOADCILA_1	1130482	1131243	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
OOADCILA_1	972752	973675	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
OOADCILA_1	973635	974585	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
OOADCILA_1	974610	975692	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
OOADCILA_1	975782	976756	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
OOADCILA_1	976762	977241	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
OOADCILA_1	980288	980887	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
OOADCILA_1	982484	983215	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
OOADCILA_1	1095125	1095718	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
OOADCILA_1	1302709	1304454	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
OOADCILA_1	1304456	1306156	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
OOADCILA_1	1307376	1308182	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
OOADCILA_1	1308182	1309123	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
OOADCILA_1	1309120	1310268	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
OOADCILA_1	977764	978597	STR_RS05230	0.0	Capsule	Immune modulation	100.00	74.53	WP_148148887
OOADCILA_1	863662	865320	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
OOADCILA_1	971323	972744	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
OOADCILA_1	983216	984670	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
OOADCILA_1	968571	969629	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
OOADCILA_1	979165	980124	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
OOADCILA_1	981024	981773	STR_RS05250	0.0	Capsule	Immune modulation	99.87	100.00	WP_011227246
OOADCILA_1	981783	982475	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
OOADCILA_1	1095125	1095718	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
OOADCILA_1	1306174	1307376	STR_RS06895	0.0	Capsule	Immune modulation	99.83	100.00	WP_011227428
OOADCILA_1	1095125	1095718	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
OOADCILA_1	1304456	1306156	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
OOADCILA_1	1306174	1307376	STER_RS07055	0.0	Capsule	Immune modulation	99.58	100.00	WP_011681436
OOADCILA_1	981783	982475	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
OOADCILA_1	1307376	1308182	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
OOADCILA_1	1308182	1309123	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
OOADCILA_1	1049069	1049859	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
OOADCILA_1	1309120	1310268	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
OOADCILA_1	1095718	1096587	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
OOADCILA_1	982484	983215	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
OOADCILA_1	981038	981773	STER_RS05305	0.0	Capsule	Immune modulation	98.37	99.33	WP_011681174
OOADCILA_1	982484	983215	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
OOADCILA_1	1302709	1304454	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
OOADCILA_1	983216	984670	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
OOADCILA_1	981038	981773	STU_RS14635	0.0	Capsule	Immune modulation	97.83	99.33	WP_011226046
OOADCILA_1	1313807	1315066	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
OOADCILA_1	983216	984670	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
OOADCILA_1	981783	982475	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
OOADCILA_1	607060	608363	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
OOADCILA_1	1596893	1597903	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
OOADCILA_1	1307376	1308182	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
OOADCILA_1	159041	160324	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
OOADCILA_1	1093650	1094696	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
OOADCILA_1	1313112	1313810	STER_RS07095	2.93e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
OOADCILA_1	978873	979109	STR_RS05235*	6.61e-119	Capsule	Immune modulation	100.00	100.00	-
OOADCILA_1	1312292	1312758	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
OOADCILA_1	1306639	1307376	SGO_RS05025	0.0	Capsule	Immune modulation	85.09	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OOADCILA_1	1133930	1134913	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
OOADCILA_1	717549	718622	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
OOADCILA_1	524824	525792	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
OOADCILA_1	1477371	1479674	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
OOADCILA_1	738073	740319	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
OOADCILA_1	1216416	1217897	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
OOADCILA_1	73298	74290	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
OOADCILA_1	27124	28095	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
OOADCILA_1	1363541	1362390	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
OOADCILA_1	1329068	1327086	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
OOADCILA_1	1117736	1116006	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
OOADCILA_1	1786459	1784981	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
OOADCILA_1	607060	608361	Eno	0.0	enolase	86.21	99.77	ABP90468
OOADCILA_1	1771722	1769830	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
OOADCILA_1	743244	741535	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
OOADCILA_1	1638585	1639595	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
OOADCILA_1	890670	889309	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
OOADCILA_1	1688916	1687024	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
OOADCILA_1	592630	593520	bglB	2.83e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
OOADCILA_1	802644	801709	SP_0676	7.16e-168	transcription facot	81.73	96.89	AAK74821
OOADCILA_1	740511	741368	metF	8.39e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
OOADCILA_1	1021370	1020666	covR	3.40e-130	responder protein	84.68	100.00	AAK71319
OOADCILA_1	1187114	1186443	rr01	2.86e-125	response regulator	84.38	99.56	CAB54566
OOADCILA_1	1021370	1020666	walR	2.44e-120	DNA-binding response regulator	80.85	100.00	ANC99504
OOADCILA_1	311719	312405	CovR	9.76e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
OOADCILA_1	747197	747847	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
OOADCILA_1	747197	747847	ciaR	6.91e-114	two component system response regulator	83.87	96.02	AIK75708
OOADCILA_1	74587	74982	SpxA2	3.59e-74	transcriptional regulator	90.91	100.00	ERL21461
OOADCILA_1	902028	902429	SpxA1	3.65e-66	transcriptional regulator	80.60	100.00	ERL19273
OOADCILA_1	160482	160982	RopE (M5005_Spy_1611)	9.58e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
OOADCILA_1	1477371	1479668	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
OOADCILA_1	1674455	1673436	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OOADCILA_1	0.0	1.0	0.0
OOADCILA_2	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
OOADCILA_1	56654	61908	False	Siphoviridae	VOG8353, VOG8336, VOG4632, VOG4609, VOG0735