safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
17	11	5	20.86

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
HENNKPBF_1	2735244	2736098	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
HENNKPBF_1	2716535	2717737	blt	ARO:3003006	blt	100.00	100.00	L32599.1
HENNKPBF_1	2494218	2495387	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
HENNKPBF_1	1376421	1376738	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
HENNKPBF_1	1376083	1376421	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
HENNKPBF_1	916374	918944	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
HENNKPBF_1	604335	605978	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
HENNKPBF_1	338756	339349	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
HENNKPBF_1	288253	289686	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
HENNKPBF_1	275438	276358	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
HENNKPBF_2	10193	10918	ANT(4')-Ia	ARO:3002623	ANT(4')-Ia	99.59	95.26	K02551.1
HENNKPBF_2	4730	7930	acrB	ARO:3000216	acrB	100.00	101.62	U00096.3
HENNKPBF_2	1278	2138	TEM-1	ARO:3000873	TEM-1	100.00	100.00	AL513383.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
HENNKPBF_2	10193	10954	aadD	Amikacin, Tobramycin	100.00	100.00	M19465
HENNKPBF_1	2735244	2736098	aadK	Streptomycin	100.00	100.00	M26879
HENNKPBF_2	1278	2138	blaTEM-1A	Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin	100.00	100.00	HM749966
HENNKPBF_2	9572	9970	bleO	Bleomycin	100.00	100.00	AF051917
HENNKPBF_1	275438	276358	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
HENNKPBF_1	4187244	4188620	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
HENNKPBF_2	10196	10963	aadD1	aminoglycoside O-nucleotidyltransferase ANT(4')-Ia	EXACTX	100.00	100.00	WP_001795128.1
HENNKPBF_1	2735247	2736098	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
HENNKPBF_2	1278	2135	blaTEM-1	broad-spectrum class A beta-lactamase TEM-1	ALLELEX	100.00	100.00	WP_000027057.1
HENNKPBF_2	9575	9976	bleO	bleomycin binding protein	EXACTX	100.00	100.00	WP_001242578.1
HENNKPBF_1	275438	276355	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
HENNKPBF_1	4184726	4185244	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
HENNKPBF_1	4187247	4188620	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
HENNKPBF_1	604338	605978	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
HENNKPBF_1	2050901	2053495	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
HENNKPBF_1	3697383	3698525	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
HENNKPBF_1	3699008	3700189	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
HENNKPBF_1	3698544	3698993	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
HENNKPBF_1	2004243	2006006	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
HENNKPBF_1	3291073	3291858	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
HENNKPBF_1	3287237	3288175	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
HENNKPBF_1	3289851	3291047	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
HENNKPBF_1	3288203	3289822	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
HENNKPBF_1	3280081	3287217	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
HENNKPBF_1	2294866	2295507	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
HENNKPBF_2	4751	7872	acrB	AcrAB	Antimicrobial activity/Competitive advantage	83.14	98.89	WP_014228207

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
HENNKPBF_2	7813	4757	AcrB	0.0	tripartite complex	99.51	97.14	AAN42062
HENNKPBF_2	7813	4757	acrB	0.0	acriflavine resistance protein	91.36	97.14	CDO13174
HENNKPBF_1	132474	133610	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
HENNKPBF_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
HENNKPBF_1	1689685	1690461	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
HENNKPBF_1	3545796	3546365	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
HENNKPBF_2	1	633	repUS12	633 / 876	100.00	GQ900379
HENNKPBF_2	2553	2683	ColRNAI	131 / 130	87.02	DQ298019

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
HENNKPBF_1	0.0	1.0	0.0
HENNKPBF_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
HENNKPBF_1	1320136	1346244	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
HENNKPBF_1	2666607	2696851	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)