Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 22 | 34 | 40.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EANJBDGJ_30 | 58332 | 59795 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.93 | 100.00 | AF274302 |
| EANJBDGJ_30 | 56995 | 58212 | mef(A) | Erythromycin, Azithromycin | 99.92 | 100.00 | U83667 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EANJBDGJ_30 | 56995 | 58209 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| EANJBDGJ_30 | 58332 | 59792 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 99.79 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EANJBDGJ_11 | 295412 | 296263 | rfbD | 0.0 | Capsule | Immune modulation | 95.19 | 100.00 | WP_014621820 |
| EANJBDGJ_5 | 25541 | 27001 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.98 | 100.14 | WP_011226049 |
| EANJBDGJ_5 | 25541 | 27001 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| EANJBDGJ_11 | 297364 | 298635 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.47 | 99.76 | WP_011226331 |
| EANJBDGJ_5 | 25541 | 27001 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| EANJBDGJ_11 | 303402 | 304211 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 93.09 | 100.00 | WP_002948020 |
| EANJBDGJ_5 | 21933 | 23300 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.06 | 100.00 | WP_011226045 |
| EANJBDGJ_11 | 306468 | 308252 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| EANJBDGJ_5 | 21935 | 23300 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 92.90 | 99.85 | WP_011681173 |
| EANJBDGJ_11 | 302462 | 303418 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| EANJBDGJ_30 | 207996 | 208589 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.42 | 100.00 | WP_011226149 |
| EANJBDGJ_11 | 301317 | 302465 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.34 | 100.00 | WP_041828290 |
| EANJBDGJ_30 | 207996 | 208589 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| EANJBDGJ_30 | 207996 | 208589 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| EANJBDGJ_11 | 304211 | 305416 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.12 | 100.00 | WP_011226326 |
| EANJBDGJ_5 | 24809 | 25540 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| EANJBDGJ_30 | 207127 | 207996 | rfbA | 0.0 | Capsule | Immune modulation | 91.61 | 100.00 | WP_011681286 |
| EANJBDGJ_5 | 24809 | 25540 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| EANJBDGJ_11 | 305437 | 306471 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| EANJBDGJ_5 | 24809 | 25540 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| EANJBDGJ_5 | 23358 | 24098 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| EANJBDGJ_22 | 311154 | 312811 | fbp54 | 0.0 | FBPs | Adherence | 90.36 | 99.88 | WP_011227179 |
| EANJBDGJ_5 | 23358 | 24098 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| EANJBDGJ_5 | 23363 | 24098 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| EANJBDGJ_30 | 36142 | 37445 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| EANJBDGJ_10 | 14309 | 15319 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| EANJBDGJ_18 | 25 | 918 | cbpD | 0.0 | Autolysin | Exoenzyme | 86.98 | 93.40 | WP_082302295 |
| EANJBDGJ_5 | 24108 | 24800 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.45 | 99.86 | WP_011226047 |
| EANJBDGJ_5 | 24108 | 24800 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.16 | 99.86 | WP_011227247 |
| EANJBDGJ_5 | 24108 | 24800 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.87 | 99.86 | WP_011681175 |
| EANJBDGJ_30 | 208792 | 209838 | rfbB | 0.0 | Capsule | Immune modulation | 84.54 | 99.90 | WP_002263087 |
| EANJBDGJ_22 | 169510 | 170271 | srtA | 0.0 | Sortase A | Exoenzyme | 83.77 | 99.74 | - |
| EANJBDGJ_31 | 1827 | 3110 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EANJBDGJ_22 | 166821 | 165838 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| EANJBDGJ_22 | 178406 | 179479 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| EANJBDGJ_11 | 113559 | 111253 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| EANJBDGJ_36 | 12045 | 13013 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| EANJBDGJ_22 | 17033 | 15552 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| EANJBDGJ_22 | 197464 | 199710 | metE | 0.0 | methionine biosynthesis enzymes | 92.12 | 100.00 | AAK74738 |
| EANJBDGJ_12 | 4620 | 3649 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| EANJBDGJ_22 | 112546 | 119094 | PrtA | 0.0 | serine protease | 90.35 | 101.73 | ABJ55268 |
| EANJBDGJ_30 | 180301 | 182031 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| EANJBDGJ_11 | 282952 | 284940 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| EANJBDGJ_11 | 254474 | 255625 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| EANJBDGJ_32 | 176815 | 175337 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| EANJBDGJ_30 | 36142 | 37443 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| EANJBDGJ_32 | 161466 | 159574 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| EANJBDGJ_22 | 202624 | 200915 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| EANJBDGJ_4 | 34934 | 36025 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| EANJBDGJ_32 | 28514 | 29524 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EANJBDGJ_22 | 328373 | 327012 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| EANJBDGJ_30 | 21736 | 22626 | bglB | 1.76e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| EANJBDGJ_22 | 242557 | 241607 | SP_0676 | 4.52e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| EANJBDGJ_22 | 199896 | 200753 | metF | 1.53e-168 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| EANJBDGJ_1 | 26946 | 27650 | covR | 1.04e-133 | responder protein | 86.38 | 100.00 | AAK71319 |
| EANJBDGJ_22 | 75339 | 76010 | rr01 | 1.19e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| EANJBDGJ_22 | 207290 | 207961 | ciaR | 3.40e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| EANJBDGJ_22 | 207290 | 207958 | CiaR | 6.27e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| EANJBDGJ_1 | 26946 | 27650 | walR | 1.71e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EANJBDGJ_31 | 161918 | 162604 | CovR | 4.75e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| EANJBDGJ_4 | 36199 | 36594 | SpxA2 | 3.61e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| EANJBDGJ_22 | 339848 | 340249 | SpxA1 | 6.72e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| EANJBDGJ_35 | 6229 | 5816 | GrpE | 1.50e-67 | protein grpE | 81.16 | 80.59 | ABP89267 |
| EANJBDGJ_31 | 185124 | 185411 | spr0479 | 4.60e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| EANJBDGJ_34 | 51392 | 51646 | spr1327 | 3.80e-45 | essential gene | 92.94 | 100.00 | AAL00131 |
| EANJBDGJ_32 | 117757 | 118020 | spr0175 | 1.05e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| EANJBDGJ_32 | 118583 | 118849 | spr0177 | 1.46e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| EANJBDGJ_31 | 3277 | 3768 | RopE (M5005_Spy_1611) | 1.05e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| EANJBDGJ_11 | 113559 | 111259 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| EANJBDGJ_32 | 65204 | 64185 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EANJBDGJ_1 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_2 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_3 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_4 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_5 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_6 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_7 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_8 | 1.0 | 0.0 | 1.0 |
| EANJBDGJ_9 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_10 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_11 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_12 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_14 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_15 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_17 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_18 | 1.0 | 0.0 | 1.0 |
| EANJBDGJ_19 | 1.0 | 0.0 | 1.0 |
| EANJBDGJ_20 | 1.0 | 0.0 | 1.0 |
| EANJBDGJ_22 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_30 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_31 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_32 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_33 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_34 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_35 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_36 | 0.0 | 1.0 | 0.0 |
| EANJBDGJ_37 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)