Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DNCMPOJM_3 | 580435 | 581289 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| DNCMPOJM_3 | 551153 | 552355 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| DNCMPOJM_2 | 426162 | 426479 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DNCMPOJM_2 | 425824 | 426162 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| DNCMPOJM_4 | 412358 | 413986 | vmlR | ARO:3004476 | vmlR | 99.08 | 99.09 | NC_000964.3 |
| DNCMPOJM_3 | 359311 | 360480 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| DNCMPOJM_5 | 262578 | 265148 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| DNCMPOJM_4 | 152885 | 153478 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| DNCMPOJM_4 | 102425 | 103864 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.42 | JYFL01000006.1 |
| DNCMPOJM_4 | 89615 | 90535 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DNCMPOJM_3 | 580437 | 581289 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| DNCMPOJM_4 | 89615 | 90535 | mph(K) | Spiramycin, Telithromycin | 98.70 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DNCMPOJM_4 | 412361 | 413986 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.08 | 98.90 | WP_003234144.1 |
| DNCMPOJM_4 | 89615 | 90532 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| DNCMPOJM_3 | 580438 | 581289 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| DNCMPOJM_3 | 58123 | 60720 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.29 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DNCMPOJM_1 | 518402 | 519583 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| DNCMPOJM_3 | 159669 | 160309 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 99.84 | NP_390062 |
| DNCMPOJM_1 | 111982 | 113178 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.50 | 100.00 | NP_391079 |
| DNCMPOJM_1 | 517938 | 518387 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| DNCMPOJM_1 | 516777 | 517919 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| DNCMPOJM_1 | 113204 | 113989 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| DNCMPOJM_3 | 8558 | 10321 | dep/capD | 0.0 | Capsule | Immune modulation | 98.64 | 100.00 | NP_389723 |
| DNCMPOJM_1 | 110334 | 111953 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391078 |
| DNCMPOJM_1 | 102212 | 109348 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.53 | 100.00 | NP_391076 |
| DNCMPOJM_1 | 109368 | 110306 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DNCMPOJM_6 | 31766 | 32902 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| DNCMPOJM_8 | 11241 | 10372 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DNCMPOJM_2 | 754670 | 755446 | CodY | 8.48e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DNCMPOJM_1 | 376868 | 377437 | ClpP | 6.34e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DNCMPOJM_2 | 274845 | 275237 | spxA1 | 6.66e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DNCMPOJM_2 | 274845 | 275237 | spx | 3.97e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DNCMPOJM_7 | 20639 | 20881 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DNCMPOJM_4 | 355086 | 355283 | CspA | 1.91e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DNCMPOJM_1 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_2 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_3 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_4 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_5 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_6 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_7 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_8 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_9 | 0.0 | 1.0 | 0.0 |
| DNCMPOJM_12 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DNCMPOJM_2 | 369495 | 396297 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)