Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 28 | 36.24 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BHHMMAKK_1 | 513454 | 514169 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.09 | 99.86 | WP_011681442 |
| BHHMMAKK_1 | 510179 | 511030 | rfbD | 0.0 | Capsule | Immune modulation | 94.84 | 100.00 | WP_014621820 |
| BHHMMAKK_1 | 1148321 | 1149052 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.67 | 100.00 | WP_011227248 |
| BHHMMAKK_1 | 518583 | 519389 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 94.18 | 100.00 | WP_011681437 |
| BHHMMAKK_1 | 518583 | 519389 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 94.18 | 100.00 | WP_011227429 |
| BHHMMAKK_1 | 1148321 | 1149052 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.99 | 100.00 | WP_011226048 |
| BHHMMAKK_1 | 1148321 | 1149052 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.72 | 100.00 | WP_011681176 |
| BHHMMAKK_1 | 514515 | 515289 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 93.29 | 99.74 | WP_011681440 |
| BHHMMAKK_1 | 511149 | 512407 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.70 | 99.92 | WP_011681445 |
| BHHMMAKK_1 | 519389 | 520591 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 92.27 | 100.00 | WP_011681436 |
| BHHMMAKK_1 | 519389 | 520591 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 92.02 | 100.00 | WP_011227428 |
| BHHMMAKK_1 | 1149053 | 1150513 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.33 | 99.86 | WP_011681177 |
| BHHMMAKK_1 | 517642 | 518583 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011681438 |
| BHHMMAKK_1 | 1296768 | 1297361 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| BHHMMAKK_1 | 1029401 | 1031059 | fbp54 | 0.0 | FBPs | Adherence | 91.02 | 99.94 | WP_011227179 |
| BHHMMAKK_1 | 517642 | 518583 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227430 |
| BHHMMAKK_1 | 1296768 | 1297361 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| BHHMMAKK_1 | 1296768 | 1297361 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| BHHMMAKK_1 | 1149053 | 1150513 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.72 | 100.00 | WP_011226049 |
| BHHMMAKK_1 | 520609 | 522194 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 90.31 | 93.06 | WP_041827753 |
| BHHMMAKK_1 | 520609 | 522194 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 90.06 | 93.06 | WP_041827077 |
| BHHMMAKK_1 | 1149053 | 1150513 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 90.03 | 100.00 | WP_041827043 |
| BHHMMAKK_1 | 1147620 | 1148312 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| BHHMMAKK_1 | 1147620 | 1148312 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| BHHMMAKK_1 | 1147620 | 1148312 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| BHHMMAKK_1 | 516497 | 517645 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 89.30 | 100.00 | WP_011227431 |
| BHHMMAKK_1 | 516497 | 517645 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 89.12 | 100.00 | WP_011681439 |
| BHHMMAKK_1 | 431223 | 432062 | cbpD | 0.0 | Autolysin | Exoenzyme | 88.94 | 99.88 | WP_011226733 |
| BHHMMAKK_1 | 522269 | 524014 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 88.72 | 99.94 | WP_011227426 |
| BHHMMAKK_1 | 522269 | 524014 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 88.32 | 99.94 | WP_011681434 |
| BHHMMAKK_1 | 1463253 | 1464556 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| BHHMMAKK_1 | 1910093 | 1911106 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| BHHMMAKK_1 | 518583 | 519389 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 84.63 | 100.00 | WP_012000440 |
| BHHMMAKK_1 | 873559 | 874320 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| BHHMMAKK_1 | 1295452 | 1296498 | rfbB | 0.0 | Capsule | Immune modulation | 83.59 | 99.90 | WP_002263087 |
| BHHMMAKK_1 | 137112 | 138395 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| BHHMMAKK_1 | 1297361 | 1298230 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| BHHMMAKK_1 | 1146940 | 1147610 | STER_RS05305 | 2.69e-148 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011681174 |
| BHHMMAKK_1 | 1146940 | 1147610 | STU_RS14635 | 2.69e-148 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011226046 |
| BHHMMAKK_1 | 1146940 | 1147610 | STR_RS05250 | 1.27e-136 | Capsule | Immune modulation | 80.09 | 89.20 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BHHMMAKK_1 | 870869 | 869886 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| BHHMMAKK_1 | 882457 | 883530 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| BHHMMAKK_1 | 1777412 | 1779718 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| BHHMMAKK_1 | 902002 | 904248 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| BHHMMAKK_1 | 1554275 | 1553307 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| BHHMMAKK_1 | 739852 | 738371 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| BHHMMAKK_1 | 25212 | 26183 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| BHHMMAKK_1 | 1323603 | 1321873 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| BHHMMAKK_1 | 498002 | 499990 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| BHHMMAKK_1 | 1630748 | 1629597 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| BHHMMAKK_1 | 2108633 | 2107155 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| BHHMMAKK_1 | 1464556 | 1463255 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| BHHMMAKK_1 | 2091871 | 2089979 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| BHHMMAKK_1 | 908585 | 906876 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| BHHMMAKK_1 | 62686 | 63777 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| BHHMMAKK_1 | 1961629 | 1962639 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| BHHMMAKK_1 | 1044810 | 1043449 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| BHHMMAKK_1 | 1478964 | 1478074 | bglB | 6.60e-176 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| BHHMMAKK_1 | 949434 | 948484 | SP_0676 | 3.13e-169 | transcription facot | 81.39 | 98.45 | AAK74821 |
| BHHMMAKK_1 | 904438 | 905295 | metF | 7.81e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| BHHMMAKK_1 | 804368 | 805039 | rr01 | 4.55e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| BHHMMAKK_1 | 1184299 | 1183595 | covR | 9.46e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| BHHMMAKK_1 | 912553 | 913224 | ciaR | 9.47e-124 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| BHHMMAKK_1 | 912553 | 913221 | CiaR | 1.18e-123 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| BHHMMAKK_1 | 296956 | 297642 | CovR | 1.78e-119 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| BHHMMAKK_1 | 1184299 | 1183595 | walR | 2.15e-119 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| BHHMMAKK_1 | 63950 | 64345 | SpxA2 | 2.09e-74 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| BHHMMAKK_1 | 1056275 | 1056676 | SpxA1 | 5.94e-68 | transcriptional regulator | 81.34 | 100.00 | ERL19273 |
| BHHMMAKK_1 | 118299 | 118712 | GrpE | 1.75e-67 | protein grpE | 82.61 | 80.59 | ABP89267 |
| BHHMMAKK_1 | 1777412 | 1779712 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| BHHMMAKK_1 | 1998044 | 1997025 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BHHMMAKK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)