Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IANMGLIB_12 | 48665 | 49711 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IANMGLIB_12 | 48668 | 49711 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.71 | 99.33 | KR610408 |
| IANMGLIB_11 | 372848 | 374224 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.51 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IANMGLIB_12 | 48668 | 49711 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| IANMGLIB_11 | 372848 | 374218 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 87.96 | 99.78 | WP_003242953.1 |
| IANMGLIB_12 | 51464 | 51982 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 81.50 | 100.00 | WP_003242546.1 |
| IANMGLIB_1 | 947067 | 949664 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IANMGLIB_10 | 514173 | 515354 | capB | 0.0 | Capsule | Immune modulation | 82.29 | 99.66 | NP_391471 |
| IANMGLIB_10 | 513709 | 514158 | capC | 7.93e-116 | Capsule | Immune modulation | 83.81 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IANMGLIB_15 | 27591 | 26455 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| IANMGLIB_3 | 12381 | 11512 | pdxS | 6.30e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| IANMGLIB_1 | 748780 | 749556 | CodY | 8.95e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IANMGLIB_1 | 260430 | 260822 | spxA1 | 1.41e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| IANMGLIB_12 | 69605 | 69408 | CspA | 5.89e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| IANMGLIB_11 | 759244 | 759050 | CspD | 2.26e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| IANMGLIB_11 | 759244 | 759047 | CspB | 4.68e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| IANMGLIB_12 | 69605 | 69411 | CspR | 2.92e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IANMGLIB_1 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_2 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_3 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_4 | 1.0 | 0.0 | 1.0 |
| IANMGLIB_7 | 1.0 | 0.0 | 1.0 |
| IANMGLIB_8 | 1.0 | 0.0 | 1.0 |
| IANMGLIB_10 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_11 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_12 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_13 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_14 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_15 | 0.0 | 1.0 | 0.0 |
| IANMGLIB_16 | 1.0 | 0.0 | 1.0 |
| IANMGLIB_17 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IANMGLIB_1 | 342867 | 370285 | False | Myoviridae | VOG4602, VOG9667, VOG5216, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG7895 |
| IANMGLIB_11 | 706224 | 708993 | False | Unknown | VOG9667, VOG9667, VOG0650, VOG4552 |
| IANMGLIB_11 | 717825 | 744193 | False | Siphoviridae | VOG2703, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230, VOG3532 |
Phigaro v2.4.0 (Database: Jan. 2024 release)