Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHHHHLDP_102 | 1700315 | 1701169 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| MHHHHLDP_102 | 1681943 | 1683145 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| MHHHHLDP_102 | 1485045 | 1486214 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MHHHHLDP_102 | 499945 | 500262 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MHHHHLDP_102 | 499607 | 499945 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MHHHHLDP_299 | 415046 | 416689 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| MHHHHLDP_403 | 260313 | 262883 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| MHHHHLDP_299 | 164520 | 165113 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| MHHHHLDP_299 | 114022 | 115455 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| MHHHHLDP_299 | 101208 | 102128 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MHHHHLDP_202 | 1019180 | 1020556 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 100.00 | D12567 |
| MHHHHLDP_102 | 1700317 | 1701169 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| MHHHHLDP_299 | 101208 | 102128 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHHHHLDP_299 | 101208 | 102125 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| MHHHHLDP_202 | 1019183 | 1020556 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| MHHHHLDP_299 | 415049 | 416689 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| MHHHHLDP_102 | 1700318 | 1701169 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| MHHHHLDP_202 | 1016709 | 1017227 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MHHHHLDP_102 | 1177270 | 1179867 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MHHHHLDP_202 | 109150 | 110088 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| MHHHHLDP_202 | 101994 | 109130 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.78 | 100.00 | NP_391076 |
| MHHHHLDP_202 | 527814 | 528995 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| MHHHHLDP_202 | 110116 | 111735 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391078 |
| MHHHHLDP_202 | 527350 | 527799 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| MHHHHLDP_202 | 112986 | 113771 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| MHHHHLDP_102 | 1126740 | 1128503 | dep/capD | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | NP_389723 |
| MHHHHLDP_202 | 111764 | 112960 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| MHHHHLDP_102 | 1285098 | 1285739 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| MHHHHLDP_202 | 526189 | 527331 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MHHHHLDP_504 | 31783 | 32919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MHHHHLDP_691 | 11191 | 10322 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MHHHHLDP_102 | 813246 | 814022 | CodY | 1.73e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MHHHHLDP_202 | 368616 | 369185 | ClpP | 6.37e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MHHHHLDP_102 | 276407 | 276799 | spxA1 | 1.36e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MHHHHLDP_102 | 276407 | 276799 | spx | 8.13e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MHHHHLDP_591 | 34656 | 34414 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MHHHHLDP_299 | 367042 | 367239 | CspA | 1.93e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MHHHHLDP_102 | 0.0 | 1.0 | 0.0 |
| MHHHHLDP_202 | 0.0 | 1.0 | 0.0 |
| MHHHHLDP_299 | 0.0 | 1.0 | 0.0 |
| MHHHHLDP_403 | 0.0 | 1.0 | 0.0 |
| MHHHHLDP_504 | 0.0 | 1.0 | 0.0 |
| MHHHHLDP_591 | 0.0 | 1.0 | 0.0 |
| MHHHHLDP_691 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MHHHHLDP_102 | 353346 | 382837 | False | Siphoviridae | VOG11003, VOG0274, VOG4600, VOG0713, VOG6430, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4705, VOG0753, VOG4574, VOG0602, VOG8440, VOG6116 |
| MHHHHLDP_102 | 442113 | 469796 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)