Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FKJBDEIH_2 | 496862 | 497200 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FKJBDEIH_2 | 496545 | 496862 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FKJBDEIH_3 | 370089 | 371009 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| FKJBDEIH_3 | 356756 | 358195 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| FKJBDEIH_3 | 307104 | 307697 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| FKJBDEIH_4 | 264187 | 265041 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| FKJBDEIH_7 | 252404 | 254974 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| FKJBDEIH_4 | 245590 | 246792 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| FKJBDEIH_4 | 56380 | 57549 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FKJBDEIH_3 | 29109 | 30752 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FKJBDEIH_4 | 264187 | 265041 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| FKJBDEIH_3 | 370089 | 371009 | mph(K) | Spiramycin, Telithromycin | 96.42 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FKJBDEIH_3 | 29109 | 30749 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| FKJBDEIH_4 | 264190 | 265041 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| FKJBDEIH_3 | 370092 | 371009 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| FKJBDEIH_5 | 200538 | 203135 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.30 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FKJBDEIH_1 | 546098 | 547279 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| FKJBDEIH_5 | 296858 | 297499 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| FKJBDEIH_1 | 544473 | 545615 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| FKJBDEIH_1 | 545634 | 546083 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FKJBDEIH_1 | 153739 | 154935 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| FKJBDEIH_1 | 154961 | 155746 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| FKJBDEIH_5 | 154839 | 156602 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| FKJBDEIH_1 | 151125 | 152063 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| FKJBDEIH_1 | 143969 | 151105 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.50 | 100.00 | NP_391076 |
| FKJBDEIH_1 | 152091 | 153710 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.10 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FKJBDEIH_10 | 31733 | 32869 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FKJBDEIH_12 | 4357 | 5226 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FKJBDEIH_2 | 185288 | 184512 | CodY | 7.44e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FKJBDEIH_1 | 404103 | 404672 | ClpP | 6.48e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FKJBDEIH_2 | 640655 | 640263 | spxA1 | 5.84e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FKJBDEIH_2 | 640655 | 640263 | spx | 3.49e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FKJBDEIH_9 | 34728 | 34486 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FKJBDEIH_3 | 80583 | 80386 | CspA | 1.98e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FKJBDEIH_5 | 308729 | 308923 | CspD | 9.22e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| FKJBDEIH_5 | 308729 | 308926 | CspB | 1.91e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FKJBDEIH_1 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_2 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_3 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_4 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_5 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_6 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_7 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_8 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_9 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_10 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_11 | 1.0 | 0.0 | 1.0 |
| FKJBDEIH_12 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_13 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_14 | 0.0 | 1.0 | 0.0 |
| FKJBDEIH_16 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FKJBDEIH_1 | 17820 | 42970 | False | Myoviridae | VOG2703, VOG1993, VOG4574, VOG0641, VOG5008, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG0815, VOG4553, VOG4555, VOG0720, VOG0796, VOG3626 |
| FKJBDEIH_2 | 527049 | 553099 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)