Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 29 | 33 | 43.93 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FLCBIABC_3 | 143249 | 144118 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| FLCBIABC_3 | 142656 | 143249 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| FLCBIABC_1 | 284261 | 286006 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681434 |
| FLCBIABC_1 | 281339 | 282541 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681436 |
| FLCBIABC_1 | 280533 | 281339 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681437 |
| FLCBIABC_1 | 279592 | 280533 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681438 |
| FLCBIABC_1 | 277552 | 278328 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681440 |
| FLCBIABC_1 | 276490 | 277206 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681442 |
| FLCBIABC_1 | 275521 | 276489 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681443 |
| FLCBIABC_1 | 274526 | 275503 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681444 |
| FLCBIABC_1 | 273270 | 274529 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| FLCBIABC_1 | 282559 | 284259 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_041827753 |
| FLCBIABC_1 | 278447 | 279595 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.91 | 100.00 | WP_011681439 |
| FLCBIABC_3 | 142656 | 143249 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| FLCBIABC_1 | 282559 | 284259 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 99.77 | 100.00 | WP_041827077 |
| FLCBIABC_3 | 11728 | 12420 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 99.71 | 100.00 | WP_011226047 |
| FLCBIABC_3 | 142656 | 143249 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| FLCBIABC_6 | 102005 | 103663 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| FLCBIABC_17 | 20677 | 21516 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| FLCBIABC_1 | 280533 | 281339 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| FLCBIABC_1 | 279592 | 280533 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | WP_011227430 |
| FLCBIABC_1 | 281339 | 282541 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011227428 |
| FLCBIABC_1 | 278447 | 279595 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | WP_011227431 |
| FLCBIABC_3 | 13161 | 14621 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 99.25 | 100.00 | WP_011681177 |
| FLCBIABC_3 | 11728 | 12420 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 99.13 | 100.00 | WP_011227247 |
| FLCBIABC_3 | 7871 | 8320 | STU_RS14620 | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | WP_011226042 |
| FLCBIABC_3 | 87753 | 88543 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.61 | 100.00 | - |
| FLCBIABC_1 | 284261 | 286006 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227426 |
| FLCBIABC_13 | 24853 | 25614 | srtA | 0.0 | Sortase A | Exoenzyme | 97.77 | 100.00 | - |
| FLCBIABC_3 | 12429 | 13160 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681176 |
| FLCBIABC_3 | 12429 | 13160 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011226048 |
| FLCBIABC_3 | 12429 | 13160 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.40 | 100.00 | WP_011227248 |
| FLCBIABC_1 | 272300 | 273151 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| FLCBIABC_3 | 13161 | 14621 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.26 | 100.21 | WP_011226049 |
| FLCBIABC_3 | 10978 | 11718 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.17 | 100.00 | WP_011226046 |
| FLCBIABC_3 | 8329 | 9325 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 96.89 | 100.00 | - |
| FLCBIABC_3 | 10978 | 11718 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| FLCBIABC_3 | 9558 | 10921 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 96.63 | 99.71 | WP_011681173 |
| FLCBIABC_3 | 13161 | 14621 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.44 | 100.21 | WP_041827043 |
| FLCBIABC_3 | 10983 | 11718 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 95.79 | 98.13 | WP_011227246 |
| FLCBIABC_3 | 9554 | 10921 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.44 | 100.00 | WP_011226045 |
| FLCBIABC_3 | 11728 | 12420 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011681175 |
| FLCBIABC_2 | 72709 | 74012 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| FLCBIABC_20 | 14315 | 15325 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| FLCBIABC_1 | 280533 | 281339 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.90 | 100.00 | WP_012000440 |
| FLCBIABC_9 | 2289 | 3572 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| FLCBIABC_3 | 141181 | 142227 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| FLCBIABC_1 | 281339 | 282076 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.96 | 60.29 | WP_012000441 |
| FLCBIABC_3 | 7549 | 7871 | STU_RS14615 | 1.00e-122 | Capsule | Immune modulation | 91.64 | 66.46 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FLCBIABC_13 | 22166 | 21183 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| FLCBIABC_12 | 15167 | 14094 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| FLCBIABC_2 | 156310 | 155342 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| FLCBIABC_1 | 116830 | 114527 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| FLCBIABC_1 | 382101 | 380620 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.71 | 100.00 | ABJ54480 |
| FLCBIABC_15 | 13862 | 16108 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| FLCBIABC_17 | 1885 | 887 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| FLCBIABC_18 | 4312 | 5283 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| FLCBIABC_1 | 230838 | 231989 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| FLCBIABC_1 | 259231 | 261219 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| FLCBIABC_13 | 38364 | 40094 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| FLCBIABC_7 | 27647 | 29125 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| FLCBIABC_2 | 74012 | 72711 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| FLCBIABC_7 | 42837 | 44729 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| FLCBIABC_15 | 19032 | 17323 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| FLCBIABC_8 | 22669 | 23679 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| FLCBIABC_6 | 80152 | 81513 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| FLCBIABC_8 | 73015 | 71123 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| FLCBIABC_2 | 88451 | 87561 | bglB | 1.21e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| FLCBIABC_10 | 11695 | 10757 | SP_0676 | 6.59e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| FLCBIABC_15 | 16300 | 17157 | metF | 2.20e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| FLCBIABC_3 | 51444 | 50740 | covR | 3.86e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| FLCBIABC_1 | 414253 | 414924 | rr01 | 7.17e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| FLCBIABC_3 | 51444 | 50740 | walR | 1.62e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| FLCBIABC_15 | 22986 | 23654 | CiaR | 2.92e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| FLCBIABC_5 | 48026 | 47340 | CovR | 6.71e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| FLCBIABC_15 | 22986 | 23657 | ciaR | 2.88e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| FLCBIABC_17 | 611 | 237 | SpxA2 | 1.37e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| FLCBIABC_6 | 68789 | 68388 | SpxA1 | 2.36e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| FLCBIABC_5 | 25647 | 25360 | spr0479 | 9.29e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| FLCBIABC_4 | 121023 | 121277 | spr1327 | 2.36e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| FLCBIABC_7 | 76462 | 76199 | spr0175 | 3.48e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| FLCBIABC_7 | 75635 | 75369 | spr0177 | 7.60e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| FLCBIABC_9 | 3730 | 4230 | RopE (M5005_Spy_1611) | 1.66e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| FLCBIABC_1 | 116830 | 114533 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| FLCBIABC_8 | 58554 | 57535 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FLCBIABC_27 | 1 | 935 | rep37 | 935 / 945 | 100.00 | HM050421 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FLCBIABC_1 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_2 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_3 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_4 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_5 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_6 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_7 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_8 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_9 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_10 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_11 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_12 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_13 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_14 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_15 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_16 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_17 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_18 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_19 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_20 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_21 | 1.0 | 0.0 | 1.0 |
| FLCBIABC_22 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_23 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_25 | 1.0 | 0.0 | 1.0 |
| FLCBIABC_26 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_27 | 1.0 | 0.0 | 1.0 |
| FLCBIABC_28 | 0.0 | 1.0 | 0.0 |
| FLCBIABC_29 | 1.0 | 0.0 | 1.0 |
| FLCBIABC_30 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)