Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 34 | 42.20 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OFMIAEGC_22 | 135341 | 135934 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| OFMIAEGC_17 | 20662 | 21501 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.88 | 100.00 | WP_011226733 |
| OFMIAEGC_22 | 135341 | 135934 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OFMIAEGC_22 | 135341 | 135934 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OFMIAEGC_22 | 371493 | 372434 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| OFMIAEGC_22 | 372431 | 373579 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_011227431 |
| OFMIAEGC_22 | 370687 | 371493 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 99.63 | 100.00 | WP_011681437 |
| OFMIAEGC_22 | 371493 | 372434 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| OFMIAEGC_22 | 372431 | 373579 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | WP_011681439 |
| OFMIAEGC_12 | 413 | 2071 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| OFMIAEGC_22 | 370687 | 371493 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011227429 |
| OFMIAEGC_22 | 366334 | 368079 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 99.37 | 100.00 | WP_011681434 |
| OFMIAEGC_22 | 135934 | 136803 | rfbA | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681286 |
| OFMIAEGC_22 | 170702 | 171463 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| OFMIAEGC_22 | 80638 | 81427 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.48 | 99.87 | - |
| OFMIAEGC_22 | 12556 | 13296 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.98 | 100.00 | WP_011681174 |
| OFMIAEGC_22 | 366334 | 368079 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 97.94 | 100.00 | WP_011227426 |
| OFMIAEGC_22 | 377118 | 378377 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 97.78 | 100.00 | WP_011681445 |
| OFMIAEGC_22 | 378498 | 379349 | rfbD | 0.0 | Capsule | Immune modulation | 97.65 | 100.00 | WP_014621820 |
| OFMIAEGC_22 | 12561 | 13296 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.42 | 98.13 | WP_011227246 |
| OFMIAEGC_22 | 14013 | 14744 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.40 | 100.00 | WP_011681176 |
| OFMIAEGC_22 | 14745 | 16205 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.33 | 100.00 | WP_011681177 |
| OFMIAEGC_22 | 12556 | 13296 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011226046 |
| OFMIAEGC_22 | 14013 | 14744 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.00 | 100.00 | WP_011226048 |
| OFMIAEGC_5 | 9572 | 14467 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.94 | 100.00 | WP_000167699 |
| OFMIAEGC_22 | 14745 | 16205 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_011226049 |
| OFMIAEGC_22 | 14745 | 16205 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.72 | 100.21 | WP_041827043 |
| OFMIAEGC_22 | 14013 | 14744 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 96.58 | 100.00 | WP_011227248 |
| OFMIAEGC_5 | 9572 | 14515 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.77 | 99.82 | YP_001200536 |
| OFMIAEGC_5 | 9572 | 14515 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.75 | 99.82 | YP_001198331 |
| OFMIAEGC_22 | 11134 | 12499 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.66 | 99.85 | WP_011681173 |
| OFMIAEGC_22 | 11132 | 12499 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.86 | 100.00 | WP_011226045 |
| OFMIAEGC_22 | 13306 | 13987 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 93.55 | 98.41 | WP_011226047 |
| OFMIAEGC_22 | 13306 | 13987 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 93.25 | 98.41 | WP_011227247 |
| OFMIAEGC_5 | 47906 | 49209 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| OFMIAEGC_16 | 14387 | 15397 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.73 | 99.51 | WP_002986042 |
| OFMIAEGC_22 | 13306 | 13987 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 86.22 | 98.41 | WP_011681175 |
| OFMIAEGC_22 | 369464 | 370687 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.52 | 99.92 | WP_012000441 |
| OFMIAEGC_22 | 370687 | 371493 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 83.02 | 100.00 | WP_012000440 |
| OFMIAEGC_24 | 62946 | 64229 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| OFMIAEGC_22 | 133866 | 134912 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| OFMIAEGC_22 | 376423 | 377121 | STER_RS07095 | 8.03e-153 | Capsule | Immune modulation | 81.15 | 70.35 | WP_011681444 |
| OFMIAEGC_22 | 1461 | 2044 | SPD_RS01775 | 1.35e-145 | Capsule | Immune modulation | 82.91 | 96.20 | WP_001819836 |
| OFMIAEGC_22 | 369950 | 370687 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 98.10 | 61.35 | WP_011227428 |
| OFMIAEGC_22 | 369950 | 370687 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 97.83 | 61.35 | WP_011681436 |
| OFMIAEGC_22 | 375603 | 376069 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.44 | 65.13 | WP_011681442 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OFMIAEGC_5 | 14488 | 9575 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.34 | 99.70 | AUS90958 |
| OFMIAEGC_22 | 174150 | 175133 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| OFMIAEGC_27 | 14393 | 13320 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| OFMIAEGC_5 | 132817 | 131849 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| OFMIAEGC_25 | 40775 | 43078 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| OFMIAEGC_13 | 11948 | 9702 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| OFMIAEGC_22 | 273350 | 274831 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.70 | 100.00 | ABJ54480 |
| OFMIAEGC_17 | 1872 | 874 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| OFMIAEGC_8 | 15447 | 14476 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| OFMIAEGC_22 | 419118 | 417967 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| OFMIAEGC_22 | 391120 | 389132 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| OFMIAEGC_22 | 157954 | 156224 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| OFMIAEGC_1 | 27366 | 28844 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| OFMIAEGC_5 | 49209 | 47908 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| OFMIAEGC_1 | 44009 | 45901 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| OFMIAEGC_13 | 6777 | 8486 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| OFMIAEGC_9 | 54953 | 53943 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| OFMIAEGC_4 | 24057 | 22705 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.90 | 98.91 | ABP90420 |
| OFMIAEGC_9 | 4688 | 6580 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| OFMIAEGC_5 | 63641 | 62751 | bglB | 4.95e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| OFMIAEGC_12 | 63414 | 64352 | SP_0676 | 3.39e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| OFMIAEGC_13 | 9510 | 8653 | metF | 8.28e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| OFMIAEGC_22 | 53006 | 52302 | covR | 2.83e-131 | responder protein | 85.11 | 100.00 | AAK71319 |
| OFMIAEGC_22 | 232082 | 231411 | rr01 | 3.34e-125 | response regulator | 84.38 | 99.56 | CAB54566 |
| OFMIAEGC_22 | 53006 | 52302 | walR | 1.42e-121 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| OFMIAEGC_13 | 2823 | 2155 | CiaR | 2.30e-121 | two-component response regulator | 85.20 | 99.55 | AAK74935 |
| OFMIAEGC_13 | 2823 | 2152 | ciaR | 2.64e-119 | two component system response regulator | 83.04 | 99.12 | AIK75708 |
| OFMIAEGC_23 | 46535 | 45849 | CovR | 7.50e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| OFMIAEGC_17 | 598 | 203 | SpxA2 | 4.38e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| OFMIAEGC_4 | 35422 | 35823 | SpxA1 | 2.00e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| OFMIAEGC_23 | 25515 | 25228 | spr0479 | 1.06e-48 | essential gene | 86.46 | 98.97 | AAK99283 |
| OFMIAEGC_5 | 167491 | 167237 | spr1327 | 2.61e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| OFMIAEGC_1 | 77623 | 77360 | spr0175 | 4.40e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| OFMIAEGC_1 | 76820 | 76530 | spr0177 | 2.58e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| OFMIAEGC_24 | 62788 | 62288 | RopE (M5005_Spy_1611) | 1.85e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| OFMIAEGC_25 | 40775 | 43072 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| OFMIAEGC_9 | 19094 | 20113 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OFMIAEGC_1 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_2 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_3 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_4 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_5 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_6 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_7 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_8 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_9 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_10 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_11 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_12 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_13 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_14 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_15 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_16 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_17 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_19 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_20 | 1.0 | 0.0 | 1.0 |
| OFMIAEGC_21 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_22 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_23 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_24 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_25 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_26 | 0.0 | 1.0 | 0.0 |
| OFMIAEGC_27 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)