Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 8 | 10 | 4 | 13.42 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCNMDKLP_1 | 1673131 | 1673448 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| MCNMDKLP_1 | 1672793 | 1673131 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| MCNMDKLP_1 | 1051883 | 1054453 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MCNMDKLP_1 | 265419 | 266852 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCNMDKLP_1 | 252628 | 253448 | mph(K) | Spiramycin, Telithromycin | 97.32 | 89.14 | NC_000964 |
| MCNMDKLP_1 | 2959563 | 2960080 | aadK | Streptomycin | 98.64864864864865 | 60.58 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCNMDKLP_1 | 684459 | 686099 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| MCNMDKLP_1 | 705694 | 707334 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| MCNMDKLP_1 | 4672162 | 4672680 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MCNMDKLP_1 | 2959565 | 2960080 | aadK | aminoglycoside 6-adenylyltransferase AadK | PARTIALX | 97.09 | 60.56 | WP_003229862.1 |
| MCNMDKLP_1 | 2967681 | 2968196 | aadK | aminoglycoside 6-adenylyltransferase AadK | PARTIALX | 97.09 | 60.56 | WP_003229862.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCNMDKLP_1 | 3646972 | 3647910 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| MCNMDKLP_1 | 4120467 | 4121648 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MCNMDKLP_1 | 4118842 | 4119984 | capA | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391469 |
| MCNMDKLP_1 | 4120003 | 4120452 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| MCNMDKLP_1 | 3650797 | 3651582 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MCNMDKLP_1 | 2309392 | 2311155 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| MCNMDKLP_1 | 3639816 | 3646952 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.78 | 100.00 | NP_391076 |
| MCNMDKLP_1 | 3649575 | 3650771 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| MCNMDKLP_1 | 2474805 | 2475446 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| MCNMDKLP_1 | 3647938 | 3649557 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCNMDKLP_1 | 138326 | 139462 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| MCNMDKLP_1 | 18651 | 19520 | pdxS | 6.11e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| MCNMDKLP_1 | 2062613 | 2063389 | CodY | 3.81e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MCNMDKLP_1 | 3978020 | 3978589 | ClpP | 2.83e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCNMDKLP_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCNMDKLP_1 | 1547355 | 1558026 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749 |
| MCNMDKLP_1 | 1586821 | 1596734 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685 |
| MCNMDKLP_1 | 1623256 | 1643581 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG0753 |
Phigaro v2.4.0 (Database: Jan. 2024 release)