safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
10	10	4	14.70

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BCPMMAOI_1	2511778	2512632	aadK	ARO:3002627	aadK	98.59	100.00	AL009126.1
BCPMMAOI_1	2494681	2495883	blt	ARO:3003006	blt	98.00	100.00	L32599.1
BCPMMAOI_1	1388307	1388624	ykkD	ARO:3003064	ykkD	98.10	100.00	AL009126.1
BCPMMAOI_1	1387969	1388307	ykkC	ARO:3003063	ykkC	98.21	100.00	AL009126.1
BCPMMAOI_1	896444	899014	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	99.53	100.00	AL009126.3
BCPMMAOI_1	601621	603267	vmlR	ARO:3004476	vmlR	98.36	100.18	NC_000964.3
BCPMMAOI_1	332498	333091	tmrB	ARO:3003059	tmrB	99.49	100.00	AL009126.3
BCPMMAOI_1	283447	284880	lmrB	ARO:3002813	lmrB	99.79	100.00	JYFL01000006.1
BCPMMAOI_1	270632	271552	mphK	ARO:3004541	mphK	98.68	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BCPMMAOI_1	2511778	2512632	aadK	Streptomycin	98.83	100.00	M26879
BCPMMAOI_1	270632	271552	mph(K)	Spiramycin, Telithromycin	98.05	100.00	NC_000964

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BCPMMAOI_1	270632	271540	mphK	macrolide 2'-phosphotransferase MphK	BLASTX	98.68	99.02	WP_003246254.1
BCPMMAOI_1	2511781	2512632	aadK	aminoglycoside 6-adenylyltransferase AadK	BLASTX	98.59	100.00	WP_003229862.1
BCPMMAOI_1	601624	603267	vmlR	ABC-F type ribosomal protection protein VmlR	BLASTX	98.36	100.00	WP_003234144.1
BCPMMAOI_1	4089226	4089744	satA	streptothricin N-acetyltransferase SatA	BLASTX	90.75	100.00	WP_003242546.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BCPMMAOI_1	3582591	3583772	capB	Capsule	Immune modulation	99.66	100.00	NP_391471
BCPMMAOI_1	3068265	3069050	dhbA	Bacillibactin	Nutritional/Metabolic factor	99.49	100.00	NP_391080
BCPMMAOI_1	3580966	3582108	capA	Capsule	Immune modulation	99.30	100.00	NP_391469
BCPMMAOI_1	3057273	3064409	dhbF	Bacillibactin	Nutritional/Metabolic factor	99.30	100.00	NP_391076
BCPMMAOI_1	1967226	1968989	dep/capD	Capsule	Immune modulation	99.04	100.00	NP_389723
BCPMMAOI_1	3064429	3065367	dhbB	Bacillibactin	Nutritional/Metabolic factor	99.04	100.00	NP_391077
BCPMMAOI_1	3067043	3068239	dhbC	Bacillibactin	Nutritional/Metabolic factor	99.00	100.00	NP_391079
BCPMMAOI_1	2099717	2100358	hlyIII	Hemolysin III	Exotoxin	98.91	100.00	NP_390062
BCPMMAOI_1	3582127	3582576	capC	Capsule	Immune modulation	98.89	100.00	NP_391470
BCPMMAOI_1	3065395	3067014	dhbE	Bacillibactin	Nutritional/Metabolic factor	96.73	100.00	NP_391078

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BCPMMAOI_1	132417	133553	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BCPMMAOI_1	18600	19469	pdxS	5.36e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.45	98.31	ACE61268
BCPMMAOI_1	1698716	1699492	CodY	3.34e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
BCPMMAOI_1	3425234	3425803	ClpP	2.48e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BCPMMAOI_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BCPMMAOI_1	1329504	1358505	False	Myoviridae	VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
BCPMMAOI_1	1824126	1854187	False	Siphoviridae	VOG4552, VOG0650, VOG0535, VOG1936, VOG1227, VOG4606, VOG2752, VOG7372, VOG0198, VOG4841, VOG7403, VOG4581, VOG4544, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG5221, VOG0136
BCPMMAOI_1	3262046	3300573	True	Siphoviridae	VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275
BCPMMAOI_1	3390893	3402051	False	Siphoviridae	VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801
BCPMMAOI_1	4026183	4034862	False	Unknown	VOG6422, VOG3239, VOG7443, VOG0944, VOG5532

Phigaro v2.4.0 (Database: Jan. 2024 release)