Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DLNOEJAJ_28 | 454604 | 455806 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DLNOEJAJ_33 | 439955 | 440272 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DLNOEJAJ_28 | 438023 | 438877 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| DLNOEJAJ_18 | 408952 | 410121 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DLNOEJAJ_12 | 108290 | 109210 | mphK | ARO:3004541 | mphK | 99.67 | 100.00 | CP010314.1 |
| DLNOEJAJ_12 | 94956 | 96395 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| DLNOEJAJ_12 | 45363 | 45956 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DLNOEJAJ_7 | 27231 | 29768 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 98.60 | 98.71 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DLNOEJAJ_12 | 108290 | 109210 | mph(K) | Spiramycin, Telithromycin | 99.13 | 100.00 | NC_000964 |
| DLNOEJAJ_1 | 328624 | 330000 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.62 | 100.00 | D12567 |
| DLNOEJAJ_28 | 438023 | 438875 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DLNOEJAJ_12 | 108302 | 109210 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.67 | 99.02 | WP_003246254.1 |
| DLNOEJAJ_1 | 328627 | 330000 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| DLNOEJAJ_28 | 438023 | 438874 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| DLNOEJAJ_1 | 326139 | 326657 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| DLNOEJAJ_18 | 60756 | 63353 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DLNOEJAJ_32 | 108870 | 109808 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| DLNOEJAJ_18 | 206327 | 206968 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| DLNOEJAJ_32 | 530883 | 532064 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DLNOEJAJ_32 | 101714 | 108850 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.58 | 100.00 | NP_391076 |
| DLNOEJAJ_18 | 10116 | 11879 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| DLNOEJAJ_32 | 529258 | 530400 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| DLNOEJAJ_32 | 530419 | 530868 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| DLNOEJAJ_32 | 112671 | 113456 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.86 | 100.00 | NP_391080 |
| DLNOEJAJ_32 | 109836 | 111455 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
| DLNOEJAJ_32 | 111618 | 112644 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 94.55 | 85.80 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DLNOEJAJ_4 | 31714 | 32850 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DLNOEJAJ_10 | 4306 | 5175 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DLNOEJAJ_33 | 749807 | 750583 | CodY | 7.01e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DLNOEJAJ_32 | 364635 | 365204 | ClpP | 4.17e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DLNOEJAJ_33 | 289964 | 290356 | spxA1 | 1.94e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| DLNOEJAJ_9 | 34710 | 34468 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DLNOEJAJ_5 | 82517 | 82320 | CspA | 9.57e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DLNOEJAJ_18 | 218911 | 219105 | CspD | 3.43e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DLNOEJAJ_1 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_2 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_3 | 1.0 | 0.0 | 1.0 |
| DLNOEJAJ_4 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_5 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_7 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_9 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_10 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_11 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_12 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_13 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_14 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_15 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_16 | 1.0 | 0.0 | 1.0 |
| DLNOEJAJ_17 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_18 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_20 | 1.0 | 0.0 | 1.0 |
| DLNOEJAJ_21 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_22 | 1.0 | 0.0 | 1.0 |
| DLNOEJAJ_23 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_25 | 1.0 | 0.0 | 1.0 |
| DLNOEJAJ_27 | 1.0 | 0.0 | 1.0 |
| DLNOEJAJ_28 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_29 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_30 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_31 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_32 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_33 | 0.0 | 1.0 | 0.0 |
| DLNOEJAJ_34 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DLNOEJAJ_18 | 95563 | 116946 | False | Siphoviridae | VOG0641, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0083, VOG5910, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG1183, VOG4841 |
| DLNOEJAJ_23 | 82719 | 100837 | False | Siphoviridae | VOG3390, VOG4705, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG5042, VOG9712 |
| DLNOEJAJ_33 | 383779 | 409945 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)