Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKCAOKJA_1 | 3959978 | 3960898 | mphK | ARO:3004541 | mphK | 96.08 | 100.00 | CP010314.1 |
| AKCAOKJA_1 | 3946654 | 3948087 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| AKCAOKJA_1 | 3896979 | 3897572 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| AKCAOKJA_1 | 3634914 | 3636560 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| AKCAOKJA_1 | 3345501 | 3348071 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| AKCAOKJA_1 | 2887447 | 2887785 | ykkC | ARO:3003063 | ykkC | 97.32 | 100.00 | AL009126.1 |
| AKCAOKJA_1 | 2887130 | 2887447 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| AKCAOKJA_1 | 1895408 | 1896577 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| AKCAOKJA_1 | 1695317 | 1696519 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| AKCAOKJA_1 | 1666072 | 1666926 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AKCAOKJA_1 | 187263 | 188639 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.77 | 100.00 | X08034 |
| AKCAOKJA_1 | 1666072 | 1666926 | aadK | Streptomycin | 98.60 | 100.00 | M26879 |
| AKCAOKJA_1 | 3959978 | 3960898 | mph(K) | Spiramycin, Telithromycin | 96.53 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKCAOKJA_1 | 3634914 | 3636557 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| AKCAOKJA_1 | 187263 | 188633 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 99.78 | WP_003242953.1 |
| AKCAOKJA_1 | 1666072 | 1666923 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| AKCAOKJA_1 | 3959981 | 3960898 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.08 | 100.00 | WP_003246254.1 |
| AKCAOKJA_1 | 190663 | 191181 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| AKCAOKJA_1 | 2201386 | 2203983 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AKCAOKJA_1 | 699227 | 700408 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| AKCAOKJA_1 | 1108382 | 1109167 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| AKCAOKJA_1 | 2095520 | 2096160 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 99.84 | NP_390062 |
| AKCAOKJA_1 | 700423 | 700872 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| AKCAOKJA_1 | 700891 | 702033 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| AKCAOKJA_1 | 1109193 | 1110389 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| AKCAOKJA_1 | 1113023 | 1120159 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.80 | 100.00 | NP_391076 |
| AKCAOKJA_1 | 2252757 | 2254520 | dep/capD | 0.0 | Capsule | Immune modulation | 98.64 | 100.00 | NP_389723 |
| AKCAOKJA_1 | 1110418 | 1112037 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
| AKCAOKJA_1 | 1112065 | 1113003 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AKCAOKJA_1 | 27946 | 26810 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| AKCAOKJA_1 | 141684 | 140815 | pdxS | 3.02e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| AKCAOKJA_1 | 2575708 | 2574932 | CodY | 3.30e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AKCAOKJA_1 | 856611 | 856042 | ClpP | 2.44e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AKCAOKJA_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AKCAOKJA_1 | 2917037 | 2945150 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)