Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OMKIGNGJ_2 | 437303 | 438157 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| OMKIGNGJ_39 | 29079 | 30725 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| OMKIGNGJ_3 | 17330 | 18532 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| OMKIGNGJ_50 | 9362 | 11932 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| OMKIGNGJ_34 | 9013 | 10182 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| OMKIGNGJ_7 | 6473 | 6811 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OMKIGNGJ_7 | 6156 | 6473 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OMKIGNGJ_5 | 34665 | 36041 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | X08034 |
| OMKIGNGJ_2 | 437303 | 438155 | aadK | Streptomycin | 99.06 | 99.77 | M26879 |
| OMKIGNGJ_45 | 39139 | 40058 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OMKIGNGJ_5 | 34665 | 36038 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| OMKIGNGJ_2 | 437303 | 438154 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| OMKIGNGJ_39 | 29079 | 30722 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| OMKIGNGJ_5 | 38022 | 38540 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OMKIGNGJ_9 | 73543 | 74481 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| OMKIGNGJ_16 | 14075 | 15217 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| OMKIGNGJ_16 | 12411 | 13592 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| OMKIGNGJ_16 | 13607 | 14056 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| OMKIGNGJ_64 | 22706 | 23347 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| OMKIGNGJ_9 | 77379 | 78164 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OMKIGNGJ_11 | 53384 | 55147 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| OMKIGNGJ_9 | 66387 | 73523 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.77 | 100.00 | NP_391076 |
| OMKIGNGJ_9 | 76157 | 77353 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.00 | 100.00 | NP_391079 |
| OMKIGNGJ_9 | 74509 | 76128 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OMKIGNGJ_31 | 27991 | 26855 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| OMKIGNGJ_55 | 11919 | 11050 | pdxS | 2.03e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| OMKIGNGJ_10 | 115403 | 114627 | CodY | 1.79e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OMKIGNGJ_13 | 80615 | 80046 | ClpP | 7.84e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OMKIGNGJ_38 | 51957 | 52349 | spxA1 | 5.80e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OMKIGNGJ_38 | 51957 | 52349 | spx | 3.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OMKIGNGJ_46 | 34704 | 34462 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OMKIGNGJ_39 | 71253 | 71056 | CspA | 3.25e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| OMKIGNGJ_64 | 34579 | 34773 | CspD | 2.18e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| OMKIGNGJ_14 | 31176 | 30982 | CspB | 1.59e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| OMKIGNGJ_57 | 2796 | 2989 | Col(SD853) | 194 / 194 | 97.42 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OMKIGNGJ_1 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_2 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_3 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_4 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_5 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_6 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_7 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_8 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_9 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_10 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_11 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_12 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_13 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_14 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_15 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_16 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_17 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_18 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_19 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_20 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_21 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_22 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_23 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_24 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_25 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_26 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_27 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_28 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_29 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_30 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_31 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_32 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_33 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_34 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_35 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_36 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_37 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_38 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_39 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_40 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_41 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_42 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_43 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_44 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_45 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_46 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_47 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_48 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_49 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_50 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_51 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_52 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_53 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_54 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_55 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_56 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_57 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_58 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_59 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_60 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_61 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_62 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_63 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_64 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_65 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_66 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_70 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_72 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_73 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_75 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_77 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_78 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_79 | 0.0 | 1.0 | 0.0 |
| OMKIGNGJ_80 | 1.0 | 0.0 | 1.0 |
| OMKIGNGJ_82 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OMKIGNGJ_2 | 172957 | 185600 | False | Siphoviridae | VOG4551, VOG1563, VOG0083, VOG10937, VOG7980, VOG0026, VOG0982, VOG5543, VOG1093, VOG0371, VOG3212 |
| OMKIGNGJ_2 | 190215 | 193922 | False | Myoviridae | VOG0085, VOG4561, VOG4724, VOG9091 |
| OMKIGNGJ_2 | 202422 | 230279 | False | Siphoviridae | VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0753, VOG4574, VOG0602, VOG7508, VOG6116, VOG0705 |
| OMKIGNGJ_2 | 279726 | 307806 | False | Siphoviridae | VOG2752, VOG7372, VOG4567, VOG0198, VOG10097, VOG9029, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG1266, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641 |
| OMKIGNGJ_7 | 36612 | 62731 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| OMKIGNGJ_26 | 4877 | 14195 | False | Siphoviridae | VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867, VOG7134, VOG10940 |
| OMKIGNGJ_40 | 16160 | 36849 | False | Siphoviridae | VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG0541, VOG4586, VOG0704, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG1886 |
Phigaro v2.4.0 (Database: Jan. 2024 release)