Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 19 | 33 | 38.20 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BFEMGPPN_1 | 44923 | 46842 | tet(M) | ARO:3000186 | tet(M) | 90.77 | 100.00 | AB039845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BFEMGPPN_1 | 429471 | 430688 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| BFEMGPPN_1 | 427888 | 429351 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
| BFEMGPPN_1 | 44924 | 46842 | tet(M) | Doxycycline, Tetracycline, Minocycline | 95.15 | 99.95 | JN846696 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BFEMGPPN_1 | 429474 | 430688 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| BFEMGPPN_1 | 427891 | 429351 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
| BFEMGPPN_1 | 44926 | 46842 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 98.28 | 100.00 | WP_011860841.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BFEMGPPN_5 | 64585 | 65553 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 98.45 | 100.00 | WP_011681443 |
| BFEMGPPN_5 | 62364 | 63617 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 96.73 | 99.52 | WP_011681445 |
| BFEMGPPN_5 | 65555 | 66270 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.09 | 99.86 | WP_011681442 |
| BFEMGPPN_5 | 61394 | 62245 | rfbD | 0.0 | Capsule | Immune modulation | 94.13 | 100.00 | WP_014621820 |
| BFEMGPPN_1 | 504310 | 505770 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.98 | 100.14 | WP_011226049 |
| BFEMGPPN_1 | 504310 | 505770 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| BFEMGPPN_1 | 504310 | 505770 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| BFEMGPPN_1 | 500702 | 502069 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.13 | 100.00 | WP_011226045 |
| BFEMGPPN_5 | 66616 | 67386 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 93.13 | 99.23 | WP_011681440 |
| BFEMGPPN_1 | 500704 | 502069 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 92.97 | 99.85 | WP_011681173 |
| BFEMGPPN_1 | 679797 | 680390 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.42 | 100.00 | WP_011226149 |
| BFEMGPPN_1 | 679797 | 680390 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| BFEMGPPN_1 | 679797 | 680390 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| BFEMGPPN_1 | 503578 | 504309 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.80 | 100.00 | WP_011226048 |
| BFEMGPPN_1 | 503578 | 504309 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| BFEMGPPN_1 | 680390 | 681259 | rfbA | 0.0 | Capsule | Immune modulation | 91.49 | 100.00 | WP_011681286 |
| BFEMGPPN_1 | 503578 | 504309 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| BFEMGPPN_1 | 368831 | 370485 | fbp54 | 0.0 | FBPs | Adherence | 90.94 | 99.70 | WP_011227179 |
| BFEMGPPN_1 | 502127 | 502867 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| BFEMGPPN_1 | 502127 | 502867 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| BFEMGPPN_1 | 502132 | 502867 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| BFEMGPPN_1 | 1048477 | 1049382 | cbpD | 0.0 | Autolysin | Exoenzyme | 88.30 | 93.50 | WP_082302295 |
| BFEMGPPN_1 | 857584 | 858887 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| BFEMGPPN_10 | 17528 | 18538 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| BFEMGPPN_1 | 502877 | 503569 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.45 | 99.86 | WP_011226047 |
| BFEMGPPN_1 | 502877 | 503569 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.16 | 99.86 | WP_011227247 |
| BFEMGPPN_1 | 502877 | 503569 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.87 | 99.86 | WP_011681175 |
| BFEMGPPN_1 | 678548 | 679594 | rfbB | 0.0 | Capsule | Immune modulation | 84.54 | 99.90 | WP_002263087 |
| BFEMGPPN_1 | 221281 | 222042 | srtA | 0.0 | Sortase A | Exoenzyme | 84.29 | 99.74 | - |
| BFEMGPPN_4 | 198529 | 199812 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BFEMGPPN_1 | 218592 | 217609 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| BFEMGPPN_1 | 230179 | 231252 | MntE | 0.0 | manganese homeostasis | 95.81 | 99.72 | ABF32167 |
| BFEMGPPN_3 | 104051 | 106357 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| BFEMGPPN_1 | 249236 | 251482 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| BFEMGPPN_1 | 949208 | 948240 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| BFEMGPPN_1 | 80171 | 78690 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| BFEMGPPN_7 | 3908 | 4879 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| BFEMGPPN_1 | 706639 | 704909 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| BFEMGPPN_5 | 19468 | 20619 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| BFEMGPPN_5 | 47946 | 49934 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| BFEMGPPN_2 | 25713 | 27191 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| BFEMGPPN_1 | 858887 | 857586 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| BFEMGPPN_2 | 41070 | 42962 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| BFEMGPPN_1 | 254404 | 252695 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| BFEMGPPN_7 | 43302 | 44393 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| BFEMGPPN_2 | 205391 | 204381 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| BFEMGPPN_1 | 386335 | 384974 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| BFEMGPPN_1 | 873293 | 872403 | bglB | 1.41e-177 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| BFEMGPPN_1 | 293741 | 292791 | SP_0676 | 2.20e-169 | transcription facot | 81.39 | 98.45 | AAK74821 |
| BFEMGPPN_1 | 251672 | 252529 | metF | 3.76e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| BFEMGPPN_1 | 550706 | 550002 | covR | 1.80e-132 | responder protein | 86.38 | 100.00 | AAK71319 |
| BFEMGPPN_1 | 143418 | 144089 | rr01 | 1.19e-125 | response regulator | 84.38 | 99.56 | CAB54566 |
| BFEMGPPN_1 | 258371 | 259042 | ciaR | 1.09e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| BFEMGPPN_1 | 258371 | 259039 | CiaR | 2.01e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| BFEMGPPN_1 | 550706 | 550002 | walR | 2.97e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| BFEMGPPN_4 | 55264 | 54578 | CovR | 4.40e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| BFEMGPPN_7 | 44567 | 44962 | SpxA2 | 4.43e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| BFEMGPPN_1 | 397801 | 398202 | SpxA1 | 2.15e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| BFEMGPPN_12 | 6014 | 5601 | GrpE | 1.55e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| BFEMGPPN_4 | 32058 | 31771 | spr0479 | 4.26e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| BFEMGPPN_1 | 995555 | 995301 | spr1327 | 3.37e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| BFEMGPPN_2 | 84779 | 84516 | spr0175 | 1.21e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| BFEMGPPN_2 | 83954 | 83688 | spr0177 | 2.69e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| BFEMGPPN_4 | 198362 | 197871 | RopE (M5005_Spy_1611) | 9.75e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| BFEMGPPN_3 | 104051 | 106351 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| BFEMGPPN_2 | 168712 | 169731 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BFEMGPPN_1 | 55041 | 56245 | repUS43 | 1206 / 1206 | 90.13 | CP003584 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BFEMGPPN_1 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_2 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_3 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_4 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_5 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_6 | 1.0 | 0.0 | 1.0 |
| BFEMGPPN_7 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_8 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_9 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_10 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_11 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_12 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_13 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_14 | 0.0 | 1.0 | 0.0 |
| BFEMGPPN_15 | 1.0 | 0.0 | 1.0 |
| BFEMGPPN_17 | 1.0 | 0.0 | 1.0 |
| BFEMGPPN_18 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)