Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 20 | 34 | 39.45 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NABPDDFC_5 | 29550 | 30401 | rfbD | 0.0 | Capsule | Immune modulation | 95.31 | 100.00 | WP_014621820 |
| NABPDDFC_25 | 13342 | 14073 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 94.81 | 100.00 | WP_011681176 |
| NABPDDFC_25 | 13342 | 14073 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011226048 |
| NABPDDFC_25 | 13342 | 14073 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.13 | 100.00 | WP_011227248 |
| NABPDDFC_5 | 35729 | 37485 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 93.17 | 98.43 | WP_011226325 |
| NABPDDFC_5 | 31723 | 32679 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| NABPDDFC_5 | 32663 | 33472 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.23 | 99.88 | WP_002948020 |
| NABPDDFC_5 | 33472 | 34677 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.12 | 100.00 | WP_011226326 |
| NABPDDFC_5 | 30578 | 31726 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.56 | 99.91 | WP_041828290 |
| NABPDDFC_5 | 34698 | 35732 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| NABPDDFC_8 | 59751 | 60344 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| NABPDDFC_6 | 63959 | 65617 | fbp54 | 0.0 | FBPs | Adherence | 90.96 | 99.94 | WP_011227179 |
| NABPDDFC_25 | 14074 | 15534 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 90.92 | 99.86 | WP_011681177 |
| NABPDDFC_8 | 59751 | 60344 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| NABPDDFC_8 | 59751 | 60344 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| NABPDDFC_25 | 14074 | 15534 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.78 | 100.00 | WP_011226049 |
| NABPDDFC_25 | 14074 | 15534 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| NABPDDFC_10 | 32021 | 32926 | cbpD | 0.0 | Autolysin | Exoenzyme | 89.96 | 93.50 | WP_082302295 |
| NABPDDFC_25 | 12641 | 13333 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| NABPDDFC_25 | 12641 | 13333 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| NABPDDFC_25 | 12641 | 13333 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| NABPDDFC_13 | 31126 | 32429 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| NABPDDFC_1 | 263832 | 264845 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| NABPDDFC_2 | 3086 | 3847 | srtA | 0.0 | Sortase A | Exoenzyme | 85.21 | 99.74 | - |
| NABPDDFC_8 | 60614 | 61660 | rfbB | 0.0 | Capsule | Immune modulation | 83.49 | 99.90 | WP_002263087 |
| NABPDDFC_9 | 1721 | 3004 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
| NABPDDFC_8 | 58882 | 59751 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| NABPDDFC_25 | 11961 | 12631 | STU_RS14635 | 9.31e-149 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011226046 |
| NABPDDFC_25 | 11961 | 12631 | STER_RS05305 | 4.33e-147 | Capsule | Immune modulation | 80.83 | 90.28 | WP_011681174 |
| NABPDDFC_25 | 12029 | 12631 | STR_RS05250 | 4.39e-137 | Capsule | Immune modulation | 81.46 | 80.13 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NABPDDFC_2 | 6537 | 7520 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| NABPDDFC_21 | 5087 | 6160 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| NABPDDFC_1 | 137874 | 140180 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| NABPDDFC_16 | 46837 | 45869 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| NABPDDFC_2 | 151574 | 153055 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| NABPDDFC_21 | 24145 | 26391 | metE | 0.0 | methionine biosynthesis enzymes | 93.06 | 100.00 | AAK74738 |
| NABPDDFC_23 | 3823 | 4794 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| NABPDDFC_2 | 61228 | 54755 | PrtA | 0.0 | serine protease | 91.30 | 100.61 | ABJ55268 |
| NABPDDFC_8 | 34054 | 35784 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.78 | 100.00 | AAK34199 |
| NABPDDFC_5 | 15921 | 17909 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| NABPDDFC_1 | 3873 | 2722 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| NABPDDFC_19 | 9713 | 8235 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| NABPDDFC_13 | 32429 | 31128 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| NABPDDFC_4 | 5839 | 7731 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| NABPDDFC_26 | 1894 | 803 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| NABPDDFC_11 | 1915 | 206 | PGM | 0.0 | phosphoglucomutase | 84.59 | 99.65 | ACH87041 |
| NABPDDFC_4 | 147642 | 146632 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| NABPDDFC_6 | 50033 | 51394 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| NABPDDFC_13 | 46843 | 45953 | bglB | 8.70e-179 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| NABPDDFC_11 | 43469 | 42519 | SP_0676 | 1.27e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| NABPDDFC_21 | 26581 | 27438 | metF | 1.20e-169 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| NABPDDFC_7 | 73553 | 74257 | covR | 4.69e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| NABPDDFC_2 | 85132 | 84461 | rr01 | 5.12e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| NABPDDFC_11 | 6581 | 7252 | ciaR | 8.26e-126 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| NABPDDFC_11 | 6581 | 7249 | CiaR | 1.53e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| NABPDDFC_12 | 53639 | 52953 | CovR | 6.77e-121 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| NABPDDFC_7 | 73553 | 74257 | walR | 1.07e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| NABPDDFC_26 | 629 | 234 | SpxA2 | 1.40e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| NABPDDFC_6 | 38575 | 38174 | SpxA1 | 7.77e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| NABPDDFC_15 | 57350 | 57763 | GrpE | 4.96e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| NABPDDFC_12 | 30436 | 30149 | spr0479 | 4.43e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| NABPDDFC_17 | 16542 | 16796 | spr1327 | 1.67e-46 | essential gene | 94.12 | 100.00 | AAL00131 |
| NABPDDFC_4 | 55364 | 55101 | spr0175 | 9.20e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| NABPDDFC_4 | 54539 | 54273 | spr0177 | 2.04e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| NABPDDFC_9 | 3171 | 3662 | RopE (M5005_Spy_1611) | 4.44e-73 | probable DNA-directed RNA polymerase subunit delta | 83.54 | 85.86 | AAK34603 |
| NABPDDFC_1 | 137874 | 140174 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.97 | 98.08 | MYN69560 |
| NABPDDFC_4 | 110966 | 111985 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
| NABPDDFC_6 | 88932 | 88393 | pnuC | 4.52e-96 | NAD salvage pathway importer | 85.00 | 66.91 | AAK75931 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NABPDDFC_1 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_2 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_3 | 1.0 | 0.0 | 1.0 |
| NABPDDFC_4 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_5 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_6 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_7 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_8 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_9 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_10 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_11 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_12 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_13 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_14 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_15 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_16 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_17 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_18 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_19 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_20 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_21 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_22 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_23 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_24 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_25 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_26 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_27 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_28 | 0.0 | 1.0 | 0.0 |
| NABPDDFC_30 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)