Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 18 | 33 | 37.64 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MLFHLGCP_1 | 65342 | 66805 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.93 | 100.00 | AF274302 |
| MLFHLGCP_1 | 66925 | 68141 | mef(A) | Erythromycin, Azithromycin | 99.92 | 99.92 | U83667 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MLFHLGCP_1 | 65345 | 66805 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 99.79 | 100.00 | WP_000420313.1 |
| MLFHLGCP_1 | 66928 | 68136 | mef(A) | macrolide efflux MFS transporter Mef(A) | BLASTX | 99.26 | 99.51 | WP_000417519.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MLFHLGCP_2 | 48522 | 49982 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.26 | 100.00 | WP_011681177 |
| MLFHLGCP_2 | 48522 | 49982 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| MLFHLGCP_9 | 23061 | 23777 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.79 | 100.00 | WP_011681442 |
| MLFHLGCP_2 | 48522 | 49982 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.37 | 100.21 | WP_041827043 |
| MLFHLGCP_2 | 52222 | 53587 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.88 | 99.85 | WP_011681173 |
| MLFHLGCP_2 | 52222 | 53589 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.52 | 100.00 | WP_011226045 |
| MLFHLGCP_2 | 51425 | 52165 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.06 | 100.00 | WP_011681174 |
| MLFHLGCP_9 | 18065 | 18916 | rfbD | 0.0 | Capsule | Immune modulation | 93.90 | 100.00 | WP_014621820 |
| MLFHLGCP_2 | 49983 | 50714 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011681176 |
| MLFHLGCP_2 | 49983 | 50714 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.72 | 100.00 | WP_011226048 |
| MLFHLGCP_2 | 51425 | 52165 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.12 | 100.00 | WP_011226046 |
| MLFHLGCP_2 | 49983 | 50714 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 93.03 | 100.00 | WP_011227248 |
| MLFHLGCP_2 | 51425 | 52160 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.80 | 98.13 | WP_011227246 |
| MLFHLGCP_9 | 24123 | 24893 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.75 | 99.10 | WP_011681440 |
| MLFHLGCP_6 | 110641 | 111234 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| MLFHLGCP_6 | 110641 | 111234 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.41 | 100.00 | WP_011226149 |
| MLFHLGCP_6 | 110641 | 111234 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.41 | 100.00 | WP_011226149 |
| MLFHLGCP_6 | 111234 | 112103 | rfbA | 0.0 | Capsule | Immune modulation | 91.03 | 100.00 | WP_011681286 |
| MLFHLGCP_10 | 23417 | 24256 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.62 | 99.88 | WP_011226733 |
| MLFHLGCP_2 | 159157 | 160815 | fbp54 | 0.0 | FBPs | Adherence | 90.24 | 99.94 | WP_011227179 |
| MLFHLGCP_3 | 273384 | 274394 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.84 | 99.80 | WP_002986042 |
| MLFHLGCP_2 | 50723 | 51415 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.47 | 99.86 | WP_011227247 |
| MLFHLGCP_2 | 50723 | 51415 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.47 | 99.86 | WP_011226047 |
| MLFHLGCP_1 | 92137 | 93440 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| MLFHLGCP_2 | 50723 | 51415 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.75 | 99.86 | WP_011681175 |
| MLFHLGCP_6 | 109324 | 110370 | rfbB | 0.0 | Capsule | Immune modulation | 84.06 | 99.90 | WP_002263087 |
| MLFHLGCP_2 | 314245 | 315006 | srtA | 0.0 | Sortase A | Exoenzyme | 83.90 | 99.74 | - |
| MLFHLGCP_4 | 2499 | 3782 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.93 | 99.53 | WP_000107753 |
| MLFHLGCP_2 | 61741 | 62324 | SPD_RS01775 | 2.13e-157 | Capsule | Immune modulation | 84.10 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MLFHLGCP_2 | 306115 | 305042 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| MLFHLGCP_2 | 317694 | 318668 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.00 | 99.09 | AAK75326 |
| MLFHLGCP_3 | 151332 | 153638 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| MLFHLGCP_8 | 83169 | 84650 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| MLFHLGCP_1 | 190743 | 189775 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| MLFHLGCP_2 | 287055 | 284809 | metE | 0.0 | methionine biosynthesis enzymes | 92.39 | 100.00 | AAK74738 |
| MLFHLGCP_10 | 43180 | 42209 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| MLFHLGCP_6 | 137488 | 135758 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| MLFHLGCP_3 | 5014 | 3863 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| MLFHLGCP_9 | 4811 | 6799 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| MLFHLGCP_5 | 25573 | 27051 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| MLFHLGCP_1 | 93440 | 92139 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| MLFHLGCP_5 | 40922 | 42814 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.42 | 98.90 | ABP91129 |
| MLFHLGCP_2 | 281890 | 283599 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| MLFHLGCP_10 | 2056 | 965 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| MLFHLGCP_5 | 184851 | 183841 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| MLFHLGCP_2 | 143595 | 144956 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| MLFHLGCP_1 | 107846 | 106956 | bglB | 4.67e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| MLFHLGCP_2 | 284622 | 283765 | metF | 1.65e-169 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| MLFHLGCP_2 | 242549 | 243511 | SP_0676 | 2.98e-169 | transcription facot | 80.69 | 99.69 | AAK74821 |
| MLFHLGCP_8 | 27273 | 26602 | rr01 | 1.14e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| MLFHLGCP_2 | 2243 | 2947 | covR | 1.39e-125 | responder protein | 85.11 | 100.00 | AAK71319 |
| MLFHLGCP_2 | 277922 | 277251 | ciaR | 1.63e-124 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| MLFHLGCP_2 | 277922 | 277254 | CiaR | 2.02e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| MLFHLGCP_4 | 163004 | 163690 | CovR | 4.77e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| MLFHLGCP_2 | 2243 | 2947 | walR | 2.23e-119 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| MLFHLGCP_10 | 791 | 396 | SpxA2 | 4.66e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| MLFHLGCP_2 | 132128 | 131727 | SpxA1 | 6.92e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| MLFHLGCP_11 | 6137 | 5733 | GrpE | 6.47e-68 | protein grpE | 82.96 | 78.82 | ABP89267 |
| MLFHLGCP_4 | 186214 | 186501 | spr0479 | 5.78e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| MLFHLGCP_1 | 232577 | 232323 | spr1327 | 1.71e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| MLFHLGCP_5 | 71839 | 71576 | spr0175 | 1.00e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| MLFHLGCP_5 | 71013 | 70747 | spr0177 | 1.40e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| MLFHLGCP_4 | 3949 | 4440 | RopE (M5005_Spy_1611) | 1.06e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| MLFHLGCP_3 | 151332 | 153632 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| MLFHLGCP_5 | 148407 | 149426 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MLFHLGCP_1 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_2 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_3 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_4 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_5 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_6 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_7 | 1.0 | 0.0 | 1.0 |
| MLFHLGCP_8 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_9 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_10 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_11 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_12 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_13 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_14 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_15 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_16 | 1.0 | 0.0 | 1.0 |
| MLFHLGCP_17 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_19 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_21 | 1.0 | 0.0 | 1.0 |
| MLFHLGCP_22 | 0.0 | 1.0 | 0.0 |
| MLFHLGCP_23 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)